Adhesion of <i>Escherichia Coli</i> to Nanostructured Surfaces and the Role of Type 1 Fimbriae
Bacterial fimbriae are an important virulence factor mediating adhesion to both biotic and abiotic surfaces and facilitating biofilm formation. The expression of type 1 fimbriae of <i>Escherichia coli</i> is a key virulence factor for urinary tract infections and catheter-associated urin...
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MDPI AG
2020-11-01
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| Series: | Nanomaterials |
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| Online Access: | https://www.mdpi.com/2079-4991/10/11/2247 |
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| author | Pawel Kallas Håvard J Haugen Nikolaj Gadegaard John Stormonth-Darling Mats Hulander Martin Andersson Håkon Valen |
| author_facet | Pawel Kallas Håvard J Haugen Nikolaj Gadegaard John Stormonth-Darling Mats Hulander Martin Andersson Håkon Valen |
| author_sort | Pawel Kallas |
| collection | DOAJ |
| description | Bacterial fimbriae are an important virulence factor mediating adhesion to both biotic and abiotic surfaces and facilitating biofilm formation. The expression of type 1 fimbriae of <i>Escherichia coli</i> is a key virulence factor for urinary tract infections and catheter-associated urinary tract infections, which represent the most common nosocomial infections. New strategies to reduce adhesion of bacteria to surfaces is therefore warranted. The aim of the present study was to investigate how surfaces with different nanotopography-influenced fimbriae-mediated adhesion. Surfaces with three different nanopattern surface coverages made in polycarbonate were fabricated by injection molding from electron beam lithography nanopatterned templates. The surfaces were constructed with features of approximately 40 nm width and 25 nm height with 100 nm, 250 nm, and 500 nm interspace distance, respectively. The role of fimbriae type 1-mediated adhesion was investigated using the <i>E. coli wild type BW25113</i> and Δ<i>fimA</i> (with a knockout of major pilus protein FimA) and Δ<i>fimH</i> (with a knockout of minor protein FimH) mutants. For the surfaces with nanotopography, all strains adhered least to areas with the largest interpillar distance (500 nm). For the <i>E. coli wild type</i>, no difference in adhesion between surfaces without pillars and the largest interpillar distance was observed. For the deletion mutants, increased adhesion was observed for surfaces without pillars compared to surfaces with the largest interpillar distance. The presence of a fully functional type 1 fimbria decreased the bacterial adhesion to the nanopatterned surfaces in comparison to the mutants. |
| first_indexed | 2024-03-10T14:53:29Z |
| format | Article |
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| issn | 2079-4991 |
| language | English |
| last_indexed | 2024-03-10T14:53:29Z |
| publishDate | 2020-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Nanomaterials |
| spelling | doaj.art-0bc467f945f442c9be136b898dc324112023-11-20T20:46:18ZengMDPI AGNanomaterials2079-49912020-11-011011224710.3390/nano10112247Adhesion of <i>Escherichia Coli</i> to Nanostructured Surfaces and the Role of Type 1 FimbriaePawel Kallas0Håvard J Haugen1Nikolaj Gadegaard2John Stormonth-Darling3Mats Hulander4Martin Andersson5Håkon Valen6Department of Biomaterials, Institute of Clinical Dentistry, University of Oslo, 0455 Oslo, NorwayDepartment of Biomaterials, Institute of Clinical Dentistry, University of Oslo, 0455 Oslo, NorwaySchool of Engineering, University of Glasgow, G12 8QQ Glasgow, UKSchool of Engineering, University of Glasgow, G12 8QQ Glasgow, UKDepartment of Chemistry and Chemical Engineering, Chalmers University of Technology, 412 58 Göteborg, SwedenDepartment of Chemistry and Chemical Engineering, Chalmers University of Technology, 412 58 Göteborg, SwedenNordic Institute of Dental Materials, 0855 Oslo, NorwayBacterial fimbriae are an important virulence factor mediating adhesion to both biotic and abiotic surfaces and facilitating biofilm formation. The expression of type 1 fimbriae of <i>Escherichia coli</i> is a key virulence factor for urinary tract infections and catheter-associated urinary tract infections, which represent the most common nosocomial infections. New strategies to reduce adhesion of bacteria to surfaces is therefore warranted. The aim of the present study was to investigate how surfaces with different nanotopography-influenced fimbriae-mediated adhesion. Surfaces with three different nanopattern surface coverages made in polycarbonate were fabricated by injection molding from electron beam lithography nanopatterned templates. The surfaces were constructed with features of approximately 40 nm width and 25 nm height with 100 nm, 250 nm, and 500 nm interspace distance, respectively. The role of fimbriae type 1-mediated adhesion was investigated using the <i>E. coli wild type BW25113</i> and Δ<i>fimA</i> (with a knockout of major pilus protein FimA) and Δ<i>fimH</i> (with a knockout of minor protein FimH) mutants. For the surfaces with nanotopography, all strains adhered least to areas with the largest interpillar distance (500 nm). For the <i>E. coli wild type</i>, no difference in adhesion between surfaces without pillars and the largest interpillar distance was observed. For the deletion mutants, increased adhesion was observed for surfaces without pillars compared to surfaces with the largest interpillar distance. The presence of a fully functional type 1 fimbria decreased the bacterial adhesion to the nanopatterned surfaces in comparison to the mutants.https://www.mdpi.com/2079-4991/10/11/2247nanostructured surfaceinjection moldinganti-adhesive<i>E. coli</i>fimbriaeanti-bacterial |
| spellingShingle | Pawel Kallas Håvard J Haugen Nikolaj Gadegaard John Stormonth-Darling Mats Hulander Martin Andersson Håkon Valen Adhesion of <i>Escherichia Coli</i> to Nanostructured Surfaces and the Role of Type 1 Fimbriae Nanomaterials nanostructured surface injection molding anti-adhesive <i>E. coli</i> fimbriae anti-bacterial |
| title | Adhesion of <i>Escherichia Coli</i> to Nanostructured Surfaces and the Role of Type 1 Fimbriae |
| title_full | Adhesion of <i>Escherichia Coli</i> to Nanostructured Surfaces and the Role of Type 1 Fimbriae |
| title_fullStr | Adhesion of <i>Escherichia Coli</i> to Nanostructured Surfaces and the Role of Type 1 Fimbriae |
| title_full_unstemmed | Adhesion of <i>Escherichia Coli</i> to Nanostructured Surfaces and the Role of Type 1 Fimbriae |
| title_short | Adhesion of <i>Escherichia Coli</i> to Nanostructured Surfaces and the Role of Type 1 Fimbriae |
| title_sort | adhesion of i escherichia coli i to nanostructured surfaces and the role of type 1 fimbriae |
| topic | nanostructured surface injection molding anti-adhesive <i>E. coli</i> fimbriae anti-bacterial |
| url | https://www.mdpi.com/2079-4991/10/11/2247 |
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