Effect of Amphetamine on Adult Male and Female Rats Prenatally Exposed to Methamphetamine
The aim of the present study was to examine the cross-sensitization induced by prenatal methamphetamine (MA) exposure to adult amphetamine (AMP) treatment in male and female rats. Rat mothers received a daily injection of MA (5 mg/kg) or saline throughout the gestation period. Adult male and female...
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| Format: | Article |
| Language: | English |
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Karolinum Press
2014-01-01
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| Series: | Prague Medical Report |
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| Online Access: | https://pmr.lf1.cuni.cz/115/1/0043/ |
| _version_ | 1819045743677145088 |
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| author | Romana Šlamberová Eva Macúchová Kateryna Nohejlová Andrea Štofková Jana Jurčovičová |
| author_facet | Romana Šlamberová Eva Macúchová Kateryna Nohejlová Andrea Štofková Jana Jurčovičová |
| author_sort | Romana Šlamberová |
| collection | DOAJ |
| description | The aim of the present study was to examine the cross-sensitization induced by prenatal methamphetamine (MA) exposure to adult amphetamine (AMP) treatment in male and female rats. Rat mothers received a daily injection of MA (5 mg/kg) or saline throughout the gestation period. Adult male and female offspring (prenatally MA- or saline-exposed) were administered with AMP (5 mg/kg) or saline (1 ml/kg) in adulthood. Behaviour in unknown environment was examined in open field test (Laboras), active drug-seeking behaviour in conditioned place preference test (CPP), spatial memory in the Morris water maze (MWM), and levels of corticosterone (CORT) were analyzed by enzyme immunoassay (EIA). Our data demonstrate that in Laboras test, AMP treatment in adulthood increased general locomotion (time and distance travelled) regardless of the prenatal exposure and sex, while AMP increased exploratory activity (rearing) only in prenatally MA-exposed animals. AMP induced sensitization only in male rats, but not in females when tested drug-seeking behaviour in the CPP test. In the spatial memory MWM test, AMP worsened the performance only in females, but not in males. On the other hand, males swam faster after chronic AMP treatment regardless of the prenatal drug exposure. EIA analysis of CORT levels demonstrated higher level in females in all measurement settings. In males, prenatal MA exposure and chronic adult AMP treatment decreased CORT levels. Thus, our data demonstrated that adult AMP treatment affects behaviour of adult rats, their spatial memory and stress response in sex-specific manner. The effect is also influenced by prenatal drug exposure. |
| first_indexed | 2024-12-21T10:33:26Z |
| format | Article |
| id | doaj.art-0bc567f1b70e4799b81c5bcccd750aa6 |
| institution | Directory Open Access Journal |
| issn | 1214-6994 2336-2936 |
| language | English |
| last_indexed | 2024-12-21T10:33:26Z |
| publishDate | 2014-01-01 |
| publisher | Karolinum Press |
| record_format | Article |
| series | Prague Medical Report |
| spelling | doaj.art-0bc567f1b70e4799b81c5bcccd750aa62022-12-21T19:07:08ZengKarolinum PressPrague Medical Report1214-69942336-29362014-01-011151435910.14712/23362936.2014.5Effect of Amphetamine on Adult Male and Female Rats Prenatally Exposed to MethamphetamineRomana Šlamberová0Eva Macúchová1Kateryna Nohejlová2Andrea Štofková3Jana Jurčovičová4Department of Normal, Pathological and Clinical Physiology, Third Faculty of Medicine, Charles University in Prague, Prague, Czech RepublicDepartment of Normal, Pathological and Clinical Physiology, Third Faculty of Medicine, Charles University in Prague, Prague, Czech RepublicDepartment of Normal, Pathological and Clinical Physiology, Third Faculty of Medicine, Charles University in Prague, Prague, Czech RepublicDepartment of Normal, Pathological and Clinical Physiology, Third Faculty of Medicine, Charles University in Prague, Prague, Czech RepublicDepartment of Normal, Pathological and Clinical Physiology, Third Faculty of Medicine, Charles University in Prague, Prague, Czech RepublicThe aim of the present study was to examine the cross-sensitization induced by prenatal methamphetamine (MA) exposure to adult amphetamine (AMP) treatment in male and female rats. Rat mothers received a daily injection of MA (5 mg/kg) or saline throughout the gestation period. Adult male and female offspring (prenatally MA- or saline-exposed) were administered with AMP (5 mg/kg) or saline (1 ml/kg) in adulthood. Behaviour in unknown environment was examined in open field test (Laboras), active drug-seeking behaviour in conditioned place preference test (CPP), spatial memory in the Morris water maze (MWM), and levels of corticosterone (CORT) were analyzed by enzyme immunoassay (EIA). Our data demonstrate that in Laboras test, AMP treatment in adulthood increased general locomotion (time and distance travelled) regardless of the prenatal exposure and sex, while AMP increased exploratory activity (rearing) only in prenatally MA-exposed animals. AMP induced sensitization only in male rats, but not in females when tested drug-seeking behaviour in the CPP test. In the spatial memory MWM test, AMP worsened the performance only in females, but not in males. On the other hand, males swam faster after chronic AMP treatment regardless of the prenatal drug exposure. EIA analysis of CORT levels demonstrated higher level in females in all measurement settings. In males, prenatal MA exposure and chronic adult AMP treatment decreased CORT levels. Thus, our data demonstrated that adult AMP treatment affects behaviour of adult rats, their spatial memory and stress response in sex-specific manner. The effect is also influenced by prenatal drug exposure.https://pmr.lf1.cuni.cz/115/1/0043/Prenatal drug exposureMethamphetamineAmphetamineOpen fieldConditioned place preferenceMorris water maze |
| spellingShingle | Romana Šlamberová Eva Macúchová Kateryna Nohejlová Andrea Štofková Jana Jurčovičová Effect of Amphetamine on Adult Male and Female Rats Prenatally Exposed to Methamphetamine Prague Medical Report Prenatal drug exposure Methamphetamine Amphetamine Open field Conditioned place preference Morris water maze |
| title | Effect of Amphetamine on Adult Male and Female Rats Prenatally Exposed to Methamphetamine |
| title_full | Effect of Amphetamine on Adult Male and Female Rats Prenatally Exposed to Methamphetamine |
| title_fullStr | Effect of Amphetamine on Adult Male and Female Rats Prenatally Exposed to Methamphetamine |
| title_full_unstemmed | Effect of Amphetamine on Adult Male and Female Rats Prenatally Exposed to Methamphetamine |
| title_short | Effect of Amphetamine on Adult Male and Female Rats Prenatally Exposed to Methamphetamine |
| title_sort | effect of amphetamine on adult male and female rats prenatally exposed to methamphetamine |
| topic | Prenatal drug exposure Methamphetamine Amphetamine Open field Conditioned place preference Morris water maze |
| url | https://pmr.lf1.cuni.cz/115/1/0043/ |
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