QTL mapping for seed density per silique in Brassica napus
Abstract Seed density per silique (SDPS) and valid silique length (VSL) are two important yield-influencing traits in rapeseed. SDPS has a direct or indirect effect on rapeseed yield through its effect on seed per silique. In this study, a quantitative trait locus (QTL) for SDPS was detected on chro...
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Nature Portfolio
2023-01-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-023-28066-5 |
| _version_ | 1797952527695609856 |
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| author | Jifeng Zhu Lei Lei Weirong Wang Jianxia Jiang Xirong Zhou |
| author_facet | Jifeng Zhu Lei Lei Weirong Wang Jianxia Jiang Xirong Zhou |
| author_sort | Jifeng Zhu |
| collection | DOAJ |
| description | Abstract Seed density per silique (SDPS) and valid silique length (VSL) are two important yield-influencing traits in rapeseed. SDPS has a direct or indirect effect on rapeseed yield through its effect on seed per silique. In this study, a quantitative trait locus (QTL) for SDPS was detected on chromosome A09 using the QTL-seq approach and confirmed via linkage analysis in the mapping population obtained from 4263 × 3001 cross. Furthermore, one major QTL for SDPS (qSD.A9-1) was mapped to a 401.8 kb genomic interval between SSR markers Nys9A190 and Nys9A531. In the same genomic region, a QTL (qSL.A9) linked to VSL was also detected. The phenotypic variation of qSD.A9-1 and qSL.A9 was 53.1% and 47.6%, respectively. Results of the additive and dominant effects demonstrated that the expression of genes controlling SDPS and VSL were derived from a different parent in this population. Subsequently, we identified 56 genes that included 45 specific genes with exonic (splicing) variants. Further analysis identified specific genes containing mutations that may be related to seed density as well as silique length. These genes could be used for further studies to understand the details of these traits of rapeseed. |
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| id | doaj.art-0bc5e610234c4d79a05a7618fa2cd8fb |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-04-10T22:47:47Z |
| publishDate | 2023-01-01 |
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| spelling | doaj.art-0bc5e610234c4d79a05a7618fa2cd8fb2023-01-15T12:11:17ZengNature PortfolioScientific Reports2045-23222023-01-011311810.1038/s41598-023-28066-5QTL mapping for seed density per silique in Brassica napusJifeng Zhu0Lei Lei1Weirong Wang2Jianxia Jiang3Xirong Zhou4Key Laboratory of Germplasm Innovation and Genetic Improvement of Grain and Oil Crops (Co-Construction By Ministry and Province), Ministry of Agriculture and Rural Affairs, Crop Breeding and Cultivation Research Institute, Shanghai Academy of Agricultural SciencesKey Laboratory of Germplasm Innovation and Genetic Improvement of Grain and Oil Crops (Co-Construction By Ministry and Province), Ministry of Agriculture and Rural Affairs, Crop Breeding and Cultivation Research Institute, Shanghai Academy of Agricultural SciencesKey Laboratory of Germplasm Innovation and Genetic Improvement of Grain and Oil Crops (Co-Construction By Ministry and Province), Ministry of Agriculture and Rural Affairs, Crop Breeding and Cultivation Research Institute, Shanghai Academy of Agricultural SciencesKey Laboratory of Germplasm Innovation and Genetic Improvement of Grain and Oil Crops (Co-Construction By Ministry and Province), Ministry of Agriculture and Rural Affairs, Crop Breeding and Cultivation Research Institute, Shanghai Academy of Agricultural SciencesKey Laboratory of Germplasm Innovation and Genetic Improvement of Grain and Oil Crops (Co-Construction By Ministry and Province), Ministry of Agriculture and Rural Affairs, Crop Breeding and Cultivation Research Institute, Shanghai Academy of Agricultural SciencesAbstract Seed density per silique (SDPS) and valid silique length (VSL) are two important yield-influencing traits in rapeseed. SDPS has a direct or indirect effect on rapeseed yield through its effect on seed per silique. In this study, a quantitative trait locus (QTL) for SDPS was detected on chromosome A09 using the QTL-seq approach and confirmed via linkage analysis in the mapping population obtained from 4263 × 3001 cross. Furthermore, one major QTL for SDPS (qSD.A9-1) was mapped to a 401.8 kb genomic interval between SSR markers Nys9A190 and Nys9A531. In the same genomic region, a QTL (qSL.A9) linked to VSL was also detected. The phenotypic variation of qSD.A9-1 and qSL.A9 was 53.1% and 47.6%, respectively. Results of the additive and dominant effects demonstrated that the expression of genes controlling SDPS and VSL were derived from a different parent in this population. Subsequently, we identified 56 genes that included 45 specific genes with exonic (splicing) variants. Further analysis identified specific genes containing mutations that may be related to seed density as well as silique length. These genes could be used for further studies to understand the details of these traits of rapeseed.https://doi.org/10.1038/s41598-023-28066-5 |
| spellingShingle | Jifeng Zhu Lei Lei Weirong Wang Jianxia Jiang Xirong Zhou QTL mapping for seed density per silique in Brassica napus Scientific Reports |
| title | QTL mapping for seed density per silique in Brassica napus |
| title_full | QTL mapping for seed density per silique in Brassica napus |
| title_fullStr | QTL mapping for seed density per silique in Brassica napus |
| title_full_unstemmed | QTL mapping for seed density per silique in Brassica napus |
| title_short | QTL mapping for seed density per silique in Brassica napus |
| title_sort | qtl mapping for seed density per silique in brassica napus |
| url | https://doi.org/10.1038/s41598-023-28066-5 |
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