Antifungal Susceptibility of <i>Saccharomyces cerevisiae</i> Isolated from Clinical Specimens
(1) Background: Despite being considered a non-pathogenic yeast, recently, a growing occurrence of <i>Saccharomyces cerevisiae</i> infections has been noted. There is little knowledge about the drug susceptibility of this species. Therefore, the objective of this research was to expand i...
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MDPI AG
2024-03-01
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| Series: | Pathogens |
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| Online Access: | https://www.mdpi.com/2076-0817/13/3/248 |
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| author | Aleksandra Górzyńska Kamila Kondracka Agnieszka Korzeniowska-Kowal Urszula Nawrot |
| author_facet | Aleksandra Górzyńska Kamila Kondracka Agnieszka Korzeniowska-Kowal Urszula Nawrot |
| author_sort | Aleksandra Górzyńska |
| collection | DOAJ |
| description | (1) Background: Despite being considered a non-pathogenic yeast, recently, a growing occurrence of <i>Saccharomyces cerevisiae</i> infections has been noted. There is little knowledge about the drug susceptibility of this species. Therefore, the objective of this research was to expand it and determine the drug susceptibility profile of a local collection of clinical isolates of this species. (2) Methods: This study contained 55 clinical isolates identified as <i>Saccharomyces cerevisiae</i> using the MALDI-TOF method. The susceptibility of <i>Saccharomyces cerevisiae</i> was tested to 10 antifungals (amphotericin B, flucytosine, fluconazole, voriconazole, posaconazole, micafungin, anidulafungin, caspofungin, and itraconazole) using MICRONAUT-AT tests and manogepix, a new drug, using the microdilution method according to EUCAST. (3) Results: Overall, most strains were classified as sensitive to amphotericin B and flucytosine (MIC ranges of ≤0.03–1 and ≤0.06–0.125, respectively) and also to echinocandins. However, five isolates expressed high MIC values for all of the tested azoles, indicating cross-resistance. The MIC range for manogepix was 0.001–0.125 mg/L, with an MIC<sub>50</sub> of 0.03 mg/L and an MIC<sub>90</sub> of 0.06 mg/L. (4) Conclusions: The occurrence of resistance to azoles may be a concerning problem and therefore should be investigated further. However, the new antifungal manogepix appears to be an interesting new therapeutic option for treating such infections. |
| first_indexed | 2024-04-24T17:55:45Z |
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| issn | 2076-0817 |
| language | English |
| last_indexed | 2024-04-24T17:55:45Z |
| publishDate | 2024-03-01 |
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| record_format | Article |
| series | Pathogens |
| spelling | doaj.art-0bc805b6af99407bbb87a03f77cbe1582024-03-27T13:58:57ZengMDPI AGPathogens2076-08172024-03-0113324810.3390/pathogens13030248Antifungal Susceptibility of <i>Saccharomyces cerevisiae</i> Isolated from Clinical SpecimensAleksandra Górzyńska0Kamila Kondracka1Agnieszka Korzeniowska-Kowal2Urszula Nawrot3Department of Pharmaceutical Microbiology and Parasitology, Wroclaw Medical University, 50-556 Wroclaw, PolandDepartment of Pharmaceutical Microbiology and Parasitology, Wroclaw Medical University, 50-556 Wroclaw, PolandDepartment of Immunology of Infectious Diseases, Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, St. Weigla 12, 53-114 Wroclaw, PolandDepartment of Pharmaceutical Microbiology and Parasitology, Wroclaw Medical University, 50-556 Wroclaw, Poland(1) Background: Despite being considered a non-pathogenic yeast, recently, a growing occurrence of <i>Saccharomyces cerevisiae</i> infections has been noted. There is little knowledge about the drug susceptibility of this species. Therefore, the objective of this research was to expand it and determine the drug susceptibility profile of a local collection of clinical isolates of this species. (2) Methods: This study contained 55 clinical isolates identified as <i>Saccharomyces cerevisiae</i> using the MALDI-TOF method. The susceptibility of <i>Saccharomyces cerevisiae</i> was tested to 10 antifungals (amphotericin B, flucytosine, fluconazole, voriconazole, posaconazole, micafungin, anidulafungin, caspofungin, and itraconazole) using MICRONAUT-AT tests and manogepix, a new drug, using the microdilution method according to EUCAST. (3) Results: Overall, most strains were classified as sensitive to amphotericin B and flucytosine (MIC ranges of ≤0.03–1 and ≤0.06–0.125, respectively) and also to echinocandins. However, five isolates expressed high MIC values for all of the tested azoles, indicating cross-resistance. The MIC range for manogepix was 0.001–0.125 mg/L, with an MIC<sub>50</sub> of 0.03 mg/L and an MIC<sub>90</sub> of 0.06 mg/L. (4) Conclusions: The occurrence of resistance to azoles may be a concerning problem and therefore should be investigated further. However, the new antifungal manogepix appears to be an interesting new therapeutic option for treating such infections.https://www.mdpi.com/2076-0817/13/3/248<i>Saccharomyces cerevisiae</i>azolesechinocandinsamphotericin Bflucytosinemanogepix |
| spellingShingle | Aleksandra Górzyńska Kamila Kondracka Agnieszka Korzeniowska-Kowal Urszula Nawrot Antifungal Susceptibility of <i>Saccharomyces cerevisiae</i> Isolated from Clinical Specimens Pathogens <i>Saccharomyces cerevisiae</i> azoles echinocandins amphotericin B flucytosine manogepix |
| title | Antifungal Susceptibility of <i>Saccharomyces cerevisiae</i> Isolated from Clinical Specimens |
| title_full | Antifungal Susceptibility of <i>Saccharomyces cerevisiae</i> Isolated from Clinical Specimens |
| title_fullStr | Antifungal Susceptibility of <i>Saccharomyces cerevisiae</i> Isolated from Clinical Specimens |
| title_full_unstemmed | Antifungal Susceptibility of <i>Saccharomyces cerevisiae</i> Isolated from Clinical Specimens |
| title_short | Antifungal Susceptibility of <i>Saccharomyces cerevisiae</i> Isolated from Clinical Specimens |
| title_sort | antifungal susceptibility of i saccharomyces cerevisiae i isolated from clinical specimens |
| topic | <i>Saccharomyces cerevisiae</i> azoles echinocandins amphotericin B flucytosine manogepix |
| url | https://www.mdpi.com/2076-0817/13/3/248 |
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