In-Package Atmospheric Cold Plasma Treatment and Storage Effects on Membrane Integrity, Oxidative Stress, and Esterase Activity of <i>Listeria monocytogenes</i>
Atmospheric cold plasma (ACP) treatment can reduce bacterial pathogens in foods. Additional reduction in bacterial cells during storage after ACP treatment was previously reported. The underlying mechanisms of bacterial inactivation during ACP treatment and post-treatment storage need to be understo...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-03-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/11/3/682 |
| _version_ | 1797436244539473920 |
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| author | Barun Yadav M. S. Roopesh |
| author_facet | Barun Yadav M. S. Roopesh |
| author_sort | Barun Yadav |
| collection | DOAJ |
| description | Atmospheric cold plasma (ACP) treatment can reduce bacterial pathogens in foods. Additional reduction in bacterial cells during storage after ACP treatment was previously reported. The underlying mechanisms of bacterial inactivation during ACP treatment and post-treatment storage need to be understood. This study investigated the changes in the morpho-physiological status of <i>Listeria monocytogenes</i> on ham surfaces after post-ACP-treatment storage of 1 h, 24 h, and 7 days at 4 °C. The membrane integrity, intracellular oxidative stress, and esterase activity of <i>L. monocytogenes</i> were evaluated by flow cytometry. <i>L. monocytogenes</i> cells were under high oxidative stress conditions with slightly permeabilized membranes after 1 h of post-ACP-treatment storage according to the flow cytometry data. During the extended storage of 24 h, the percentage of cells with a slightly permeabilized membrane increased; subsequently, the percentage of cells with intact membranes decreased. The percentage of <i>L. monocytogenes</i> cells with intact membranes decreased to <5% with a treatment time of 10 min and after 7 days of post-treatment storage. In addition, the percentage of <i>L. monocytogenes</i> cells under oxidation stress decreased to <1%, whereas the percentage of cells with completely permeabilized membranes increased to more than 90% for samples treated with ACP for 10 min and 7 days of post-treatment storage. With increased ACP treatment time, for 1 h stored samples, the percentage of cells with active esterase and slightly permeabilized membranes increased. However, during the extended post-treatment storage of 7 days, the percentage of cells with active esterase and slightly permeabilized membranes decreased to below 1%. At the same time, the percentage of cells with permeabilized membrane increased to more than 92% with an increase in ACP treatment time of 10 min. In conclusion, the higher inactivation after 24 h and 7 days post-ACP-treatment storage compared to 1 h stored samples correlated with the loss of esterase activity and membrane integrity of <i>L. monocytogenes</i> cells. |
| first_indexed | 2024-03-09T10:58:51Z |
| format | Article |
| id | doaj.art-0bc89b0bc1294f45a108c2ad6dae2033 |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-09T10:58:51Z |
| publishDate | 2023-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-0bc89b0bc1294f45a108c2ad6dae20332023-12-01T01:22:37ZengMDPI AGMicroorganisms2076-26072023-03-0111368210.3390/microorganisms11030682In-Package Atmospheric Cold Plasma Treatment and Storage Effects on Membrane Integrity, Oxidative Stress, and Esterase Activity of <i>Listeria monocytogenes</i>Barun Yadav0M. S. Roopesh1Department of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton, AB T6G 2P5, CanadaDepartment of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton, AB T6G 2P5, CanadaAtmospheric cold plasma (ACP) treatment can reduce bacterial pathogens in foods. Additional reduction in bacterial cells during storage after ACP treatment was previously reported. The underlying mechanisms of bacterial inactivation during ACP treatment and post-treatment storage need to be understood. This study investigated the changes in the morpho-physiological status of <i>Listeria monocytogenes</i> on ham surfaces after post-ACP-treatment storage of 1 h, 24 h, and 7 days at 4 °C. The membrane integrity, intracellular oxidative stress, and esterase activity of <i>L. monocytogenes</i> were evaluated by flow cytometry. <i>L. monocytogenes</i> cells were under high oxidative stress conditions with slightly permeabilized membranes after 1 h of post-ACP-treatment storage according to the flow cytometry data. During the extended storage of 24 h, the percentage of cells with a slightly permeabilized membrane increased; subsequently, the percentage of cells with intact membranes decreased. The percentage of <i>L. monocytogenes</i> cells with intact membranes decreased to <5% with a treatment time of 10 min and after 7 days of post-treatment storage. In addition, the percentage of <i>L. monocytogenes</i> cells under oxidation stress decreased to <1%, whereas the percentage of cells with completely permeabilized membranes increased to more than 90% for samples treated with ACP for 10 min and 7 days of post-treatment storage. With increased ACP treatment time, for 1 h stored samples, the percentage of cells with active esterase and slightly permeabilized membranes increased. However, during the extended post-treatment storage of 7 days, the percentage of cells with active esterase and slightly permeabilized membranes decreased to below 1%. At the same time, the percentage of cells with permeabilized membrane increased to more than 92% with an increase in ACP treatment time of 10 min. In conclusion, the higher inactivation after 24 h and 7 days post-ACP-treatment storage compared to 1 h stored samples correlated with the loss of esterase activity and membrane integrity of <i>L. monocytogenes</i> cells.https://www.mdpi.com/2076-2607/11/3/682cold plasmastorage<i>Listeria</i>hamesterase activitymembrane permeabilization |
| spellingShingle | Barun Yadav M. S. Roopesh In-Package Atmospheric Cold Plasma Treatment and Storage Effects on Membrane Integrity, Oxidative Stress, and Esterase Activity of <i>Listeria monocytogenes</i> Microorganisms cold plasma storage <i>Listeria</i> ham esterase activity membrane permeabilization |
| title | In-Package Atmospheric Cold Plasma Treatment and Storage Effects on Membrane Integrity, Oxidative Stress, and Esterase Activity of <i>Listeria monocytogenes</i> |
| title_full | In-Package Atmospheric Cold Plasma Treatment and Storage Effects on Membrane Integrity, Oxidative Stress, and Esterase Activity of <i>Listeria monocytogenes</i> |
| title_fullStr | In-Package Atmospheric Cold Plasma Treatment and Storage Effects on Membrane Integrity, Oxidative Stress, and Esterase Activity of <i>Listeria monocytogenes</i> |
| title_full_unstemmed | In-Package Atmospheric Cold Plasma Treatment and Storage Effects on Membrane Integrity, Oxidative Stress, and Esterase Activity of <i>Listeria monocytogenes</i> |
| title_short | In-Package Atmospheric Cold Plasma Treatment and Storage Effects on Membrane Integrity, Oxidative Stress, and Esterase Activity of <i>Listeria monocytogenes</i> |
| title_sort | in package atmospheric cold plasma treatment and storage effects on membrane integrity oxidative stress and esterase activity of i listeria monocytogenes i |
| topic | cold plasma storage <i>Listeria</i> ham esterase activity membrane permeabilization |
| url | https://www.mdpi.com/2076-2607/11/3/682 |
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