Simultaneous Isolation and Identification of Largemouth Bass Virus and Rhabdovirus from Moribund Largemouth Bass (<i>Micropterus salmoides</i>)
Largemouth bass is an important commercially farmed fish in China, but the rapid expansion of its breeding has resulted in increased incidence of diseases caused by bacteria, viruses and parasites. In this study, moribund largemouth bass containing ulcer foci on body surfaces indicated the most like...
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MDPI AG
2022-07-01
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| Series: | Viruses |
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| Online Access: | https://www.mdpi.com/1999-4915/14/8/1643 |
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| author | Yuqi Jin Sven M. Bergmann Qianyi Mai Ying Yang Weiqiang Liu Dongli Sun Yanfeng Chen Yingying Yu Yuhong Liu Wenlong Cai Hanxu Dong Hua Li Hui Yu Yali Wu Mingjian Lai Weiwei Zeng |
| author_facet | Yuqi Jin Sven M. Bergmann Qianyi Mai Ying Yang Weiqiang Liu Dongli Sun Yanfeng Chen Yingying Yu Yuhong Liu Wenlong Cai Hanxu Dong Hua Li Hui Yu Yali Wu Mingjian Lai Weiwei Zeng |
| author_sort | Yuqi Jin |
| collection | DOAJ |
| description | Largemouth bass is an important commercially farmed fish in China, but the rapid expansion of its breeding has resulted in increased incidence of diseases caused by bacteria, viruses and parasites. In this study, moribund largemouth bass containing ulcer foci on body surfaces indicated the most likely pathogens were iridovirus and rhabdovirus members and this was confirmed using a combination of immunohistochemistry, cell culture, electron microscopy and conserved gene sequence analysis. We identified that these fish had been co-infected with these viruses. We observed bullet-shaped virions (100–140 nm long and 50–100 nm in diameter) along with hexagonal virions with 140 nm diameters in cell culture inoculated with tissue homogenates. The viruses were plaque purified and a comparison of the highly conserved regions of the genome of these viruses indicated that they are most similar to largemouth bass virus (LMBV) and hybrid snakehead rhabdovirus (HSHRV), respectively. Regression infection experiments indicated fish mortalities for LMBV-FS2021 and HSHRV-MS2021 were 86.7 and 11.1%, respectively. While co-infection resulted in 93.3% mortality that was significantly (<i>p</i> < 0.05) higher than the single infections even though the viral loads differed by >100-fold. Overall, we simultaneously isolated and identified LMBV and a HSHRV-like virus from diseased largemouth bass, and our results can provide novel ideas for the prevention and treatment of combined virus infection especially in largemouth bass. |
| first_indexed | 2024-03-09T12:22:26Z |
| format | Article |
| id | doaj.art-0bcacae50996413ebc6c33dadd5d141c |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-03-09T12:22:26Z |
| publishDate | 2022-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj.art-0bcacae50996413ebc6c33dadd5d141c2023-11-30T22:38:24ZengMDPI AGViruses1999-49152022-07-01148164310.3390/v14081643Simultaneous Isolation and Identification of Largemouth Bass Virus and Rhabdovirus from Moribund Largemouth Bass (<i>Micropterus salmoides</i>)Yuqi Jin0Sven M. Bergmann1Qianyi Mai2Ying Yang3Weiqiang Liu4Dongli Sun5Yanfeng Chen6Yingying Yu7Yuhong Liu8Wenlong Cai9Hanxu Dong10Hua Li11Hui Yu12Yali Wu13Mingjian Lai14Weiwei Zeng15Guangdong Provincial Key Laboratory of Animal Molecular Design and Precise Breeding, School of Life Science and Engineering, Foshan University, Foshan 528231, ChinaInstitute of Infectology, Friedrich-Loffler-Institut (FLI), Federal Research Institute for Animal Health, Südufer 10, 17493 Greifswald-InselRiems, GermanyGuangdong Provincial Key Laboratory of Animal Molecular Design and Precise Breeding, School of Life Science and Engineering, Foshan University, Foshan 528231, ChinaGuangdong Provincial Key Laboratory of Animal Molecular Design and Precise Breeding, School of Life Science and Engineering, Foshan University, Foshan 528231, ChinaGuangdong Provincial Key Laboratory of Animal Molecular Design and Precise Breeding, School of Life Science and Engineering, Foshan University, Foshan 528231, ChinaGuangdong Provincial Key Laboratory of Animal Molecular Design and Precise Breeding, School of Life Science and Engineering, Foshan University, Foshan 528231, ChinaGuangdong Provincial Key Laboratory of Animal Molecular Design and Precise Breeding, School of Life Science and Engineering, Foshan University, Foshan 528231, ChinaGuangdong Provincial Key Laboratory of Animal Molecular Design and Precise Breeding, School of Life Science and Engineering, Foshan University, Foshan 528231, ChinaGuangdong Provincial Key Laboratory of Animal Molecular Design and Precise Breeding, School of Life Science and Engineering, Foshan University, Foshan 528231, ChinaDepartment of Infectious Diseases and Public Health, Jockey Club College of Veterinary Medicine and Life Sciences, City University of Hong Kong, Kowloon, Hong Kong 999077, ChinaGuangdong Provincial Key Laboratory of Animal Molecular Design and Precise Breeding, School of Life Science and Engineering, Foshan University, Foshan 528231, ChinaGuangdong Provincial Key Laboratory of Animal Molecular Design and Precise Breeding, School of Life Science and Engineering, Foshan University, Foshan 528231, ChinaGuangdong Provincial Key Laboratory of Animal Molecular Design and Precise Breeding, School of Life Science and Engineering, Foshan University, Foshan 528231, ChinaFoshan Institute of Agricultural Sciences, Guangdong, Foshan 528145, ChinaFoshan Institute of Agricultural Sciences, Guangdong, Foshan 528145, ChinaGuangdong Provincial Key Laboratory of Animal Molecular Design and Precise Breeding, School of Life Science and Engineering, Foshan University, Foshan 528231, ChinaLargemouth bass is an important commercially farmed fish in China, but the rapid expansion of its breeding has resulted in increased incidence of diseases caused by bacteria, viruses and parasites. In this study, moribund largemouth bass containing ulcer foci on body surfaces indicated the most likely pathogens were iridovirus and rhabdovirus members and this was confirmed using a combination of immunohistochemistry, cell culture, electron microscopy and conserved gene sequence analysis. We identified that these fish had been co-infected with these viruses. We observed bullet-shaped virions (100–140 nm long and 50–100 nm in diameter) along with hexagonal virions with 140 nm diameters in cell culture inoculated with tissue homogenates. The viruses were plaque purified and a comparison of the highly conserved regions of the genome of these viruses indicated that they are most similar to largemouth bass virus (LMBV) and hybrid snakehead rhabdovirus (HSHRV), respectively. Regression infection experiments indicated fish mortalities for LMBV-FS2021 and HSHRV-MS2021 were 86.7 and 11.1%, respectively. While co-infection resulted in 93.3% mortality that was significantly (<i>p</i> < 0.05) higher than the single infections even though the viral loads differed by >100-fold. Overall, we simultaneously isolated and identified LMBV and a HSHRV-like virus from diseased largemouth bass, and our results can provide novel ideas for the prevention and treatment of combined virus infection especially in largemouth bass.https://www.mdpi.com/1999-4915/14/8/1643<i>Micropterus salmoides</i>largemouth bassco-infectionlargemouth bass virushybrid snakehead rhabdovirusvirus isolation |
| spellingShingle | Yuqi Jin Sven M. Bergmann Qianyi Mai Ying Yang Weiqiang Liu Dongli Sun Yanfeng Chen Yingying Yu Yuhong Liu Wenlong Cai Hanxu Dong Hua Li Hui Yu Yali Wu Mingjian Lai Weiwei Zeng Simultaneous Isolation and Identification of Largemouth Bass Virus and Rhabdovirus from Moribund Largemouth Bass (<i>Micropterus salmoides</i>) Viruses <i>Micropterus salmoides</i> largemouth bass co-infection largemouth bass virus hybrid snakehead rhabdovirus virus isolation |
| title | Simultaneous Isolation and Identification of Largemouth Bass Virus and Rhabdovirus from Moribund Largemouth Bass (<i>Micropterus salmoides</i>) |
| title_full | Simultaneous Isolation and Identification of Largemouth Bass Virus and Rhabdovirus from Moribund Largemouth Bass (<i>Micropterus salmoides</i>) |
| title_fullStr | Simultaneous Isolation and Identification of Largemouth Bass Virus and Rhabdovirus from Moribund Largemouth Bass (<i>Micropterus salmoides</i>) |
| title_full_unstemmed | Simultaneous Isolation and Identification of Largemouth Bass Virus and Rhabdovirus from Moribund Largemouth Bass (<i>Micropterus salmoides</i>) |
| title_short | Simultaneous Isolation and Identification of Largemouth Bass Virus and Rhabdovirus from Moribund Largemouth Bass (<i>Micropterus salmoides</i>) |
| title_sort | simultaneous isolation and identification of largemouth bass virus and rhabdovirus from moribund largemouth bass i micropterus salmoides i |
| topic | <i>Micropterus salmoides</i> largemouth bass co-infection largemouth bass virus hybrid snakehead rhabdovirus virus isolation |
| url | https://www.mdpi.com/1999-4915/14/8/1643 |
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