Gliding motility of Babesia bovis merozoites visualized by time-lapse video microscopy.
BACKGROUND: Babesia bovis is an apicomplexan intraerythrocytic protozoan parasite that induces babesiosis in cattle after transmission by ticks. During specific stages of the apicomplexan parasite lifecycle, such as the sporozoites of Plasmodium falciparum and tachyzoites of Toxoplasma gondii, host...
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2012-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3323635?pdf=render |
| _version_ | 1811266202949910528 |
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| author | Masahito Asada Yasuyuki Goto Kazuhide Yahata Naoaki Yokoyama Satoru Kawai Noboru Inoue Osamu Kaneko Shin-ichiro Kawazu |
| author_facet | Masahito Asada Yasuyuki Goto Kazuhide Yahata Naoaki Yokoyama Satoru Kawai Noboru Inoue Osamu Kaneko Shin-ichiro Kawazu |
| author_sort | Masahito Asada |
| collection | DOAJ |
| description | BACKGROUND: Babesia bovis is an apicomplexan intraerythrocytic protozoan parasite that induces babesiosis in cattle after transmission by ticks. During specific stages of the apicomplexan parasite lifecycle, such as the sporozoites of Plasmodium falciparum and tachyzoites of Toxoplasma gondii, host cells are targeted for invasion using a unique, active process termed "gliding motility". However, it is not thoroughly understood how the merozoites of B. bovis target and invade host red blood cells (RBCs), and gliding motility has so far not been observed in the parasite. METHODOLOGY/PRINCIPAL FINDINGS: Gliding motility of B. bovis merozoites was revealed by time-lapse video microscopy. The recorded images revealed that the process included egress of the merozoites from the infected RBC, gliding motility, and subsequent invasion into new RBCs. The gliding motility of B. bovis merozoites was similar to the helical gliding of Toxoplasma tachyzoites. The trails left by the merozoites were detected by indirect immunofluorescence assay using antiserum against B. bovis merozoite surface antigen 1. Inhibition of gliding motility by actin filament polymerization or depolymerization indicated that the gliding motility was driven by actomyosin dependent process. In addition, we revealed the timing of breakdown of the parasitophorous vacuole. Time-lapse image analysis of membrane-stained bovine RBCs showed formation and breakdown of the parasitophorous vacuole within ten minutes of invasion. CONCLUSIONS/SIGNIFICANCE: This is the first report of the gliding motility of B. bovis. Since merozoites of Plasmodium parasites do not glide on a substrate, the gliding motility of B. bovis merozoites is a notable finding. |
| first_indexed | 2024-04-12T20:37:51Z |
| format | Article |
| id | doaj.art-0bcc30432de0451f8f92b8328db0846c |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-12T20:37:51Z |
| publishDate | 2012-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-0bcc30432de0451f8f92b8328db0846c2022-12-22T03:17:30ZengPublic Library of Science (PLoS)PLoS ONE1932-62032012-01-0174e3522710.1371/journal.pone.0035227Gliding motility of Babesia bovis merozoites visualized by time-lapse video microscopy.Masahito AsadaYasuyuki GotoKazuhide YahataNaoaki YokoyamaSatoru KawaiNoboru InoueOsamu KanekoShin-ichiro KawazuBACKGROUND: Babesia bovis is an apicomplexan intraerythrocytic protozoan parasite that induces babesiosis in cattle after transmission by ticks. During specific stages of the apicomplexan parasite lifecycle, such as the sporozoites of Plasmodium falciparum and tachyzoites of Toxoplasma gondii, host cells are targeted for invasion using a unique, active process termed "gliding motility". However, it is not thoroughly understood how the merozoites of B. bovis target and invade host red blood cells (RBCs), and gliding motility has so far not been observed in the parasite. METHODOLOGY/PRINCIPAL FINDINGS: Gliding motility of B. bovis merozoites was revealed by time-lapse video microscopy. The recorded images revealed that the process included egress of the merozoites from the infected RBC, gliding motility, and subsequent invasion into new RBCs. The gliding motility of B. bovis merozoites was similar to the helical gliding of Toxoplasma tachyzoites. The trails left by the merozoites were detected by indirect immunofluorescence assay using antiserum against B. bovis merozoite surface antigen 1. Inhibition of gliding motility by actin filament polymerization or depolymerization indicated that the gliding motility was driven by actomyosin dependent process. In addition, we revealed the timing of breakdown of the parasitophorous vacuole. Time-lapse image analysis of membrane-stained bovine RBCs showed formation and breakdown of the parasitophorous vacuole within ten minutes of invasion. CONCLUSIONS/SIGNIFICANCE: This is the first report of the gliding motility of B. bovis. Since merozoites of Plasmodium parasites do not glide on a substrate, the gliding motility of B. bovis merozoites is a notable finding.http://europepmc.org/articles/PMC3323635?pdf=render |
| spellingShingle | Masahito Asada Yasuyuki Goto Kazuhide Yahata Naoaki Yokoyama Satoru Kawai Noboru Inoue Osamu Kaneko Shin-ichiro Kawazu Gliding motility of Babesia bovis merozoites visualized by time-lapse video microscopy. PLoS ONE |
| title | Gliding motility of Babesia bovis merozoites visualized by time-lapse video microscopy. |
| title_full | Gliding motility of Babesia bovis merozoites visualized by time-lapse video microscopy. |
| title_fullStr | Gliding motility of Babesia bovis merozoites visualized by time-lapse video microscopy. |
| title_full_unstemmed | Gliding motility of Babesia bovis merozoites visualized by time-lapse video microscopy. |
| title_short | Gliding motility of Babesia bovis merozoites visualized by time-lapse video microscopy. |
| title_sort | gliding motility of babesia bovis merozoites visualized by time lapse video microscopy |
| url | http://europepmc.org/articles/PMC3323635?pdf=render |
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