PvMSP8 as a Novel <i>Plasmodium vivax</i> Malaria Sero-Marker for the Peruvian Amazon
The measurement of recent malaria exposure can support malaria control efforts. This study evaluated serological responses to an in-house <i>Plasmodium vivax</i> Merozoite Surface Protein 8 (PvMSP8) expressed in a Baculovirus system as sero-marker of recent exposure to <i>P. vivax&...
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MDPI AG
2021-03-01
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| Series: | Pathogens |
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| Online Access: | https://www.mdpi.com/2076-0817/10/3/282 |
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| author | Elizabeth Villasis Katherine Garro Angel Rosas-Aguirre Pamela Rodriguez Jason Rosado Anthony Gave Mitchel Guzman-Guzman Paulo Manrique Michael White Niko Speybroeck Joseph Michael Vinetz Katherine Torres Dionicia Gamboa |
| author_facet | Elizabeth Villasis Katherine Garro Angel Rosas-Aguirre Pamela Rodriguez Jason Rosado Anthony Gave Mitchel Guzman-Guzman Paulo Manrique Michael White Niko Speybroeck Joseph Michael Vinetz Katherine Torres Dionicia Gamboa |
| author_sort | Elizabeth Villasis |
| collection | DOAJ |
| description | The measurement of recent malaria exposure can support malaria control efforts. This study evaluated serological responses to an in-house <i>Plasmodium vivax</i> Merozoite Surface Protein 8 (PvMSP8) expressed in a Baculovirus system as sero-marker of recent exposure to <i>P. vivax</i> (Pv) in the Peruvian Amazon. In a first evaluation, IgGs against PvMSP8 and PvMSP10 proteins were measured by Luminex in a cohort of 422 Amazonian individuals with known history of Pv exposure (monthly data of infection status by qPCR and/or microscopy over five months). Both serological responses were able to discriminate between exposed and non-exposed individuals in a good manner, with slightly higher performance of anti-PvMSP10 IgGs (area under the curve AUC = 0.78 [95% CI = 0.72–0.83]) than anti-PvMSP8 IgGs (AUC = 0.72 [95% CI = 0.67–0.78]) (<i>p</i> = 0.01). In a second evaluation, the analysis by ELISA of 1251 plasma samples, collected during a population-based cross-sectional survey, confirmed the good performance of anti-PvMSP8 IgGs for discriminating between individuals with Pv infection at the time of survey and/or with antecedent of Pv in the past month (AUC = 0.79 [95% CI = 0.74–0.83]). Anti-PvMSP8 IgG antibodies can be considered as a good biomarker of recent Pv exposure in low-moderate transmission settings of the Peruvian Amazon. |
| first_indexed | 2024-03-09T05:56:53Z |
| format | Article |
| id | doaj.art-0bce5cecd3ed499cb7d19a1d8c92738f |
| institution | Directory Open Access Journal |
| issn | 2076-0817 |
| language | English |
| last_indexed | 2024-03-09T05:56:53Z |
| publishDate | 2021-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pathogens |
| spelling | doaj.art-0bce5cecd3ed499cb7d19a1d8c92738f2023-12-03T12:12:22ZengMDPI AGPathogens2076-08172021-03-0110328210.3390/pathogens10030282PvMSP8 as a Novel <i>Plasmodium vivax</i> Malaria Sero-Marker for the Peruvian AmazonElizabeth Villasis0Katherine Garro1Angel Rosas-Aguirre2Pamela Rodriguez3Jason Rosado4Anthony Gave5Mitchel Guzman-Guzman6Paulo Manrique7Michael White8Niko Speybroeck9Joseph Michael Vinetz10Katherine Torres11Dionicia Gamboa12Laboratorio de Malaria, Laboratorios de Investigación y Desarrollo, Facultad de Ciencias y Filosofía, Universidad Peruana Cayetano Heredia, 15102, Lima, PeruLaboratorio de Malaria, Laboratorios de Investigación y Desarrollo, Facultad de Ciencias y Filosofía, Universidad Peruana Cayetano Heredia, 15102, Lima, PeruInstituto de Medicina Tropical Alexander von Humboldt, Universidad Peruana Cayetano Heredia, Lima 15102, PeruLaboratorio de Malaria, Laboratorios de Investigación y Desarrollo, Facultad de Ciencias y Filosofía, Universidad Peruana Cayetano Heredia, 15102, Lima, PeruMalaria: Parasites and Hosts Unit, Institut Pasteur, Paris 75015, FranceLaboratorio de Malaria: Parásitos y Vectores, Laboratorios de Investigación y Desarrollo, Facultad de Ciencias y Filosofía, Universidad Peruana Cayetano Heredia, Lima 15102, PeruLaboratorio ICEMR-Amazonia, Laboratorios de Investigación y Desarrollo, Facultad de Ciencias y Filosofía, Universidad Peruana Cayetano Heredia, Lima 15102, PeruLeishmania and Malaria Research Unit. Instituto de Medicina Tropical Alexander von Humboldt, Universidad Peruana Cayetano Heredia, Lima 15102, PeruMalaria: Parasites and Hosts Unit, Institut Pasteur, Paris 75015, FranceResearch Institute of Health and Society (IRSS). Université Catholique de Louvain, Clos Chapelle-aux-champs 30/B1.30.14 1200 Woluwe-Saint-Lambert, Brussels 1200, BelgiumInstituto de Medicina Tropical Alexander von Humboldt, Universidad Peruana Cayetano Heredia, Lima 15102, PeruLaboratorio de Malaria, Laboratorios de Investigación y Desarrollo, Facultad de Ciencias y Filosofía, Universidad Peruana Cayetano Heredia, 15102, Lima, PeruInstituto de Medicina Tropical Alexander von Humboldt, Universidad Peruana Cayetano Heredia, Lima 15102, PeruThe measurement of recent malaria exposure can support malaria control efforts. This study evaluated serological responses to an in-house <i>Plasmodium vivax</i> Merozoite Surface Protein 8 (PvMSP8) expressed in a Baculovirus system as sero-marker of recent exposure to <i>P. vivax</i> (Pv) in the Peruvian Amazon. In a first evaluation, IgGs against PvMSP8 and PvMSP10 proteins were measured by Luminex in a cohort of 422 Amazonian individuals with known history of Pv exposure (monthly data of infection status by qPCR and/or microscopy over five months). Both serological responses were able to discriminate between exposed and non-exposed individuals in a good manner, with slightly higher performance of anti-PvMSP10 IgGs (area under the curve AUC = 0.78 [95% CI = 0.72–0.83]) than anti-PvMSP8 IgGs (AUC = 0.72 [95% CI = 0.67–0.78]) (<i>p</i> = 0.01). In a second evaluation, the analysis by ELISA of 1251 plasma samples, collected during a population-based cross-sectional survey, confirmed the good performance of anti-PvMSP8 IgGs for discriminating between individuals with Pv infection at the time of survey and/or with antecedent of Pv in the past month (AUC = 0.79 [95% CI = 0.74–0.83]). Anti-PvMSP8 IgG antibodies can be considered as a good biomarker of recent Pv exposure in low-moderate transmission settings of the Peruvian Amazon.https://www.mdpi.com/2076-0817/10/3/282PvMSP8antibodies<i>P. vivax</i>LuminexELISAmalaria |
| spellingShingle | Elizabeth Villasis Katherine Garro Angel Rosas-Aguirre Pamela Rodriguez Jason Rosado Anthony Gave Mitchel Guzman-Guzman Paulo Manrique Michael White Niko Speybroeck Joseph Michael Vinetz Katherine Torres Dionicia Gamboa PvMSP8 as a Novel <i>Plasmodium vivax</i> Malaria Sero-Marker for the Peruvian Amazon Pathogens PvMSP8 antibodies <i>P. vivax</i> Luminex ELISA malaria |
| title | PvMSP8 as a Novel <i>Plasmodium vivax</i> Malaria Sero-Marker for the Peruvian Amazon |
| title_full | PvMSP8 as a Novel <i>Plasmodium vivax</i> Malaria Sero-Marker for the Peruvian Amazon |
| title_fullStr | PvMSP8 as a Novel <i>Plasmodium vivax</i> Malaria Sero-Marker for the Peruvian Amazon |
| title_full_unstemmed | PvMSP8 as a Novel <i>Plasmodium vivax</i> Malaria Sero-Marker for the Peruvian Amazon |
| title_short | PvMSP8 as a Novel <i>Plasmodium vivax</i> Malaria Sero-Marker for the Peruvian Amazon |
| title_sort | pvmsp8 as a novel i plasmodium vivax i malaria sero marker for the peruvian amazon |
| topic | PvMSP8 antibodies <i>P. vivax</i> Luminex ELISA malaria |
| url | https://www.mdpi.com/2076-0817/10/3/282 |
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