Apoptosis activation during Lagovirus europaeus/GI.2 infection in rabbits
Rabbit Haemorrhagic Disease (RHD) is a severe disease caused by Lagovirus europaeus/GI.1 and GI.2. Immunological processes such as apoptosis are important factors involved in the pathogenesis of Rabbit Haemorrhagic Disease (RHD). The process of programmed cell death has been quite well characterized...
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| Format: | Article |
| Jezik: | English |
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Frontiers Media S.A.
2024-01-01
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| Serija: | Frontiers in Microbiology |
| Teme: | |
| Online dostop: | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1308018/full |
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| author | Dominika Bębnowska Rafał Hrynkiewicz Karolina Wiśniewska Magdalena Żabińska Estera Rintz Karolina Pierzynowska Paulina Niedźwiedzka-Rystwej |
| author_facet | Dominika Bębnowska Rafał Hrynkiewicz Karolina Wiśniewska Magdalena Żabińska Estera Rintz Karolina Pierzynowska Paulina Niedźwiedzka-Rystwej |
| author_sort | Dominika Bębnowska |
| collection | DOAJ |
| description | Rabbit Haemorrhagic Disease (RHD) is a severe disease caused by Lagovirus europaeus/GI.1 and GI.2. Immunological processes such as apoptosis are important factors involved in the pathogenesis of Rabbit Haemorrhagic Disease (RHD). The process of programmed cell death has been quite well characterized in infection with GI.1 strains, but apoptosis in infection with GI.2 strains has not been widely studied. This is particularly important as several studies have shown that significant differences in the host immune response are observed during infection with different strains of Lagovirus europaeus. In this study, we analyzed the gene expression, protein levels and activity of key apoptotic cell death factors in the spleen, kidney, lung, and heart of rabbits. As a result, we showed that there is a significant increase in caspase-3, Bax, Bcl2 and Bax/Bcl2 mRNA gene expression ratio in organs of infected animals. Our results show also increased levels of cleaved caspase-3, caspase-6 and PARP. Moreover, significant activity of caspase-3 was also detected. Our results indicate that caspase-3, caspase-6 and genes coding Bcl2 family proteins play a key role in the apoptotic response in Lagovirus europaeus/GI.2 infection in organs that are not the target of virus replication. |
| first_indexed | 2024-03-08T11:33:59Z |
| format | Article |
| id | doaj.art-0bcf610259124131855795b400d25fcd |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-03-08T11:33:59Z |
| publishDate | 2024-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-0bcf610259124131855795b400d25fcd2024-01-25T13:38:18ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2024-01-011410.3389/fmicb.2023.13080181308018Apoptosis activation during Lagovirus europaeus/GI.2 infection in rabbitsDominika Bębnowska0Rafał Hrynkiewicz1Karolina Wiśniewska2Magdalena Żabińska3Estera Rintz4Karolina Pierzynowska5Paulina Niedźwiedzka-Rystwej6Institute of Biology, University of Szczecin, Szczecin, PolandInstitute of Biology, University of Szczecin, Szczecin, PolandDepartment of Molecular Biology, Faculty of Biology, University of Gdańsk, Gdańsk, PolandDepartment of Molecular Biology, Faculty of Biology, University of Gdańsk, Gdańsk, PolandDepartment of Molecular Biology, Faculty of Biology, University of Gdańsk, Gdańsk, PolandDepartment of Molecular Biology, Faculty of Biology, University of Gdańsk, Gdańsk, PolandInstitute of Biology, University of Szczecin, Szczecin, PolandRabbit Haemorrhagic Disease (RHD) is a severe disease caused by Lagovirus europaeus/GI.1 and GI.2. Immunological processes such as apoptosis are important factors involved in the pathogenesis of Rabbit Haemorrhagic Disease (RHD). The process of programmed cell death has been quite well characterized in infection with GI.1 strains, but apoptosis in infection with GI.2 strains has not been widely studied. This is particularly important as several studies have shown that significant differences in the host immune response are observed during infection with different strains of Lagovirus europaeus. In this study, we analyzed the gene expression, protein levels and activity of key apoptotic cell death factors in the spleen, kidney, lung, and heart of rabbits. As a result, we showed that there is a significant increase in caspase-3, Bax, Bcl2 and Bax/Bcl2 mRNA gene expression ratio in organs of infected animals. Our results show also increased levels of cleaved caspase-3, caspase-6 and PARP. Moreover, significant activity of caspase-3 was also detected. Our results indicate that caspase-3, caspase-6 and genes coding Bcl2 family proteins play a key role in the apoptotic response in Lagovirus europaeus/GI.2 infection in organs that are not the target of virus replication.https://www.frontiersin.org/articles/10.3389/fmicb.2023.1308018/fullLagovirus europaeusrabbit haemorrhagic diseaseRHDVapoptosisrabbits |
| spellingShingle | Dominika Bębnowska Rafał Hrynkiewicz Karolina Wiśniewska Magdalena Żabińska Estera Rintz Karolina Pierzynowska Paulina Niedźwiedzka-Rystwej Apoptosis activation during Lagovirus europaeus/GI.2 infection in rabbits Frontiers in Microbiology Lagovirus europaeus rabbit haemorrhagic disease RHDV apoptosis rabbits |
| title | Apoptosis activation during Lagovirus europaeus/GI.2 infection in rabbits |
| title_full | Apoptosis activation during Lagovirus europaeus/GI.2 infection in rabbits |
| title_fullStr | Apoptosis activation during Lagovirus europaeus/GI.2 infection in rabbits |
| title_full_unstemmed | Apoptosis activation during Lagovirus europaeus/GI.2 infection in rabbits |
| title_short | Apoptosis activation during Lagovirus europaeus/GI.2 infection in rabbits |
| title_sort | apoptosis activation during lagovirus europaeus gi 2 infection in rabbits |
| topic | Lagovirus europaeus rabbit haemorrhagic disease RHDV apoptosis rabbits |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1308018/full |
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