汉麻籽抗氧化肽的制备与氨基酸序列分析 Preparation and amino acid sequence analysis of antioxidant peptides from hemp seeds

汉麻籽抗氧化肽的制备与氨基酸序列分析 Preparation and amino acid sequence analysis of antioxidant peptides from hemp seeds

为开发具有较好抗氧化活性的植物源蛋白肽，采用木瓜蛋白酶和中性蛋白酶复合酶法水解汉麻籽粕获得汉麻籽蛋白水解物，经膜分离技术得到4种不同分子质量的多肽组分。通过DPPH自由基清除率、总还原力的测定，评价不同多肽组分的抗氧化活性，并对抗氧化活性最强的多肽组分进行氨基酸序列分析。结果显示：低分子质量的汉麻籽多肽组分具有更好的抗氧化活性，其中HP-Ⅳ组分（分子质量＜1 kDa）的抗氧化活性最强；HP-Ⅳ组分中丰度较高的6个肽段的分子质量分别为 364.84、539.77、630.30、662.33、718.90、827.41 Da，氨基酸序列分别为NRDDMRER、ANQLDQFSR、NPEDEFEQL...

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Bibliographic Details
Main Author:	魏连会1，董艳1，石杰1，宋淑敏1，孙兴荣2WEI Lianhui1, DONG Yan1, SHI Jie1,SONG Shumin1, SUN Xingrong2
Format:	Article
Language:	English
Published:	中粮工科（西安）国际工程有限公司 2022-04-01
Series:	Zhongguo youzhi
Subjects:	汉麻籽多肽；抗氧化活性；氨基酸序列 hemp seed polypeptides; antioxidant activity; amino acid sequence
Online Access:	http://tg.chinaoils.cn/ch/reader/create_pdf.aspx?file_no=20220407&year_id=2022&quarter_id=4&falg=1

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