Molecular Detection and Phylogenetic Characterization of <i>Anaplasma</i> spp. in Dogs from Hainan Province/Island, China
Anaplasmosis is a serious infection which is transmitted by ticks and mosquitos. There are very few reports and studies that have been carried out to understand the prevalence, distribution, and epidemiological profile of <i>Anaplasma</i> spp. infection in dogs in Hainan province/island....
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| Format: | Article |
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MDPI AG
2023-05-01
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| Series: | Veterinary Sciences |
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| Online Access: | https://www.mdpi.com/2306-7381/10/5/339 |
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| author | Yang Lin Sa Zhou Archana Upadhyay Jianguo Zhao Chenghong Liao Qingfeng Guan Jinhua Wang Qian Han |
| author_facet | Yang Lin Sa Zhou Archana Upadhyay Jianguo Zhao Chenghong Liao Qingfeng Guan Jinhua Wang Qian Han |
| author_sort | Yang Lin |
| collection | DOAJ |
| description | Anaplasmosis is a serious infection which is transmitted by ticks and mosquitos. There are very few reports and studies that have been carried out to understand the prevalence, distribution, and epidemiological profile of <i>Anaplasma</i> spp. infection in dogs in Hainan province/island. In the present study, we have tried to understand the prevalence, distribution, and occurrence of <i>Anaplasma</i> spp. infections in dogs (<i>n</i> = 1051) in Hainan Island/Province to establish a surveillance-based study. The confirmed positive samples by Polymerase chain reaction (PCR) were subjected to capillary sequencing for further strain-specific confirmation, followed by the construction of phylogenetic trees to determine their genetic relations. Various statistical tools were used to analyze related risk factors. There were three species of <i>Anaplasma</i> detected from the Hainan region; namely, <i>A. phagocytophilum</i>, <i>A. bovis</i>, and <i>A. platys</i>. The overall prevalence of <i>Anaplasma</i> is 9.7% (102/1051). <i>A. phagocytopihum</i> was prevalent in 1.0% of dogs (11/1051), <i>A. bovis</i> was found in 2.7% of dogs (28/1051), and <i>A. platys</i> in 6.0% of dogs (63/1051). Our surveillance-based study conducted to understand the occurrence and distribution pattern of <i>Anaplasma</i> spp. in Hainan will help in designing effective control measures along with management strategies so as to treat and control the infection in the area. |
| first_indexed | 2024-03-11T03:14:04Z |
| format | Article |
| id | doaj.art-0bd105895447432e9be779796c297795 |
| institution | Directory Open Access Journal |
| issn | 2306-7381 |
| language | English |
| last_indexed | 2024-03-11T03:14:04Z |
| publishDate | 2023-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Veterinary Sciences |
| spelling | doaj.art-0bd105895447432e9be779796c2977952023-11-18T03:37:31ZengMDPI AGVeterinary Sciences2306-73812023-05-0110533910.3390/vetsci10050339Molecular Detection and Phylogenetic Characterization of <i>Anaplasma</i> spp. in Dogs from Hainan Province/Island, ChinaYang Lin0Sa Zhou1Archana Upadhyay2Jianguo Zhao3Chenghong Liao4Qingfeng Guan5Jinhua Wang6Qian Han7One Health Institute, Hainan University, Haikou 570228, ChinaOne Health Institute, Hainan University, Haikou 570228, ChinaOne Health Institute, Hainan University, Haikou 570228, ChinaOne Health Institute, Hainan University, Haikou 570228, ChinaOne Health Institute, Hainan University, Haikou 570228, ChinaOne Health Institute, Hainan University, Haikou 570228, ChinaOne Health Institute, Hainan University, Haikou 570228, ChinaOne Health Institute, Hainan University, Haikou 570228, ChinaAnaplasmosis is a serious infection which is transmitted by ticks and mosquitos. There are very few reports and studies that have been carried out to understand the prevalence, distribution, and epidemiological profile of <i>Anaplasma</i> spp. infection in dogs in Hainan province/island. In the present study, we have tried to understand the prevalence, distribution, and occurrence of <i>Anaplasma</i> spp. infections in dogs (<i>n</i> = 1051) in Hainan Island/Province to establish a surveillance-based study. The confirmed positive samples by Polymerase chain reaction (PCR) were subjected to capillary sequencing for further strain-specific confirmation, followed by the construction of phylogenetic trees to determine their genetic relations. Various statistical tools were used to analyze related risk factors. There were three species of <i>Anaplasma</i> detected from the Hainan region; namely, <i>A. phagocytophilum</i>, <i>A. bovis</i>, and <i>A. platys</i>. The overall prevalence of <i>Anaplasma</i> is 9.7% (102/1051). <i>A. phagocytopihum</i> was prevalent in 1.0% of dogs (11/1051), <i>A. bovis</i> was found in 2.7% of dogs (28/1051), and <i>A. platys</i> in 6.0% of dogs (63/1051). Our surveillance-based study conducted to understand the occurrence and distribution pattern of <i>Anaplasma</i> spp. in Hainan will help in designing effective control measures along with management strategies so as to treat and control the infection in the area.https://www.mdpi.com/2306-7381/10/5/339<i>Anaplasma</i>dogsHainanco-infectionprevalence |
| spellingShingle | Yang Lin Sa Zhou Archana Upadhyay Jianguo Zhao Chenghong Liao Qingfeng Guan Jinhua Wang Qian Han Molecular Detection and Phylogenetic Characterization of <i>Anaplasma</i> spp. in Dogs from Hainan Province/Island, China Veterinary Sciences <i>Anaplasma</i> dogs Hainan co-infection prevalence |
| title | Molecular Detection and Phylogenetic Characterization of <i>Anaplasma</i> spp. in Dogs from Hainan Province/Island, China |
| title_full | Molecular Detection and Phylogenetic Characterization of <i>Anaplasma</i> spp. in Dogs from Hainan Province/Island, China |
| title_fullStr | Molecular Detection and Phylogenetic Characterization of <i>Anaplasma</i> spp. in Dogs from Hainan Province/Island, China |
| title_full_unstemmed | Molecular Detection and Phylogenetic Characterization of <i>Anaplasma</i> spp. in Dogs from Hainan Province/Island, China |
| title_short | Molecular Detection and Phylogenetic Characterization of <i>Anaplasma</i> spp. in Dogs from Hainan Province/Island, China |
| title_sort | molecular detection and phylogenetic characterization of i anaplasma i spp in dogs from hainan province island china |
| topic | <i>Anaplasma</i> dogs Hainan co-infection prevalence |
| url | https://www.mdpi.com/2306-7381/10/5/339 |
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