Characterization and Antibacterial Activity Assessment of Hydroxyapatite-Betel Leaf Extract Formulation against <i>Streptococcus mutans In Vitro</i> and <i>In Vivo</i>
Hydroxyapatite is an inorganic material that is commonly used as a re-mineralizing agent. Adding natural ingredients such as green betel leaf can increase the antibacterial properties due to the presence of phenolic compounds, flavonoids, and tannins. This study aims to determine the physical and ch...
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| Format: | Article |
| Language: | English |
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Department of Chemistry, Universitas Gadjah Mada
2023-03-01
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| Series: | Indonesian Journal of Chemistry |
| Subjects: | |
| Online Access: | https://jurnal.ugm.ac.id/ijc/article/view/77853 |
| _version_ | 1827965519814197248 |
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| author | Fitriari Izzatunnisa Muhaimin Sari Edi Cahyaningrum Riska Amelia Lawarti Dina Kartika Maharani |
| author_facet | Fitriari Izzatunnisa Muhaimin Sari Edi Cahyaningrum Riska Amelia Lawarti Dina Kartika Maharani |
| author_sort | Fitriari Izzatunnisa Muhaimin |
| collection | DOAJ |
| description | Hydroxyapatite is an inorganic material that is commonly used as a re-mineralizing agent. Adding natural ingredients such as green betel leaf can increase the antibacterial properties due to the presence of phenolic compounds, flavonoids, and tannins. This study aims to determine the physical and chemical characteristics of the formulation of hydroxyapatite-betel leaf extract and the antibacterial activity against Streptococcus mutans. To characterize the combination of hydroxyapatite-betel leaf extract, XRD, PSA and FTIR analyses were performed. Particle size analysis showed the smallest results in the variation of betel 0.3 g, which is 690.08 nm. FTIR characterization showed the presence of OH, PO43− and CO32− functional groups from hydroxyapatite and C=O derived from betel leaf extract. In addition, in vitro and in vivo analyses were performed to assess the antibacterial activity of this formulation. The in vitro antibacterial activity test against S. mutans showed strong inhibitory activity. Our finding suggests that the formulation has the potential to be used as a medication or prevention agent for dental caries. |
| first_indexed | 2024-04-09T17:34:16Z |
| format | Article |
| id | doaj.art-0bd44d8ca35345ad9689dd1f329ab0ca |
| institution | Directory Open Access Journal |
| issn | 1411-9420 2460-1578 |
| language | English |
| last_indexed | 2024-04-09T17:34:16Z |
| publishDate | 2023-03-01 |
| publisher | Department of Chemistry, Universitas Gadjah Mada |
| record_format | Article |
| series | Indonesian Journal of Chemistry |
| spelling | doaj.art-0bd44d8ca35345ad9689dd1f329ab0ca2023-04-18T01:55:50ZengDepartment of Chemistry, Universitas Gadjah MadaIndonesian Journal of Chemistry1411-94202460-15782023-03-0123235836910.22146/ijc.7785333428Characterization and Antibacterial Activity Assessment of Hydroxyapatite-Betel Leaf Extract Formulation against <i>Streptococcus mutans In Vitro</i> and <i>In Vivo</i>Fitriari Izzatunnisa Muhaimin0Sari Edi Cahyaningrum1Riska Amelia Lawarti2Dina Kartika Maharani3Department of Biology, Faculty of Science and Mathematics, Universitas Negeri Surabaya, Jl. Ketintang, Surabaya 60231, IndonesiaDepartment of Chemistry, Faculty of Science and Mathematics, Universitas Negeri Surabaya, Jl. Ketintang, Surabaya 60231, IndonesiaDepartment of Chemistry, Faculty of Science and Mathematics, Universitas Negeri Surabaya, Jl. Ketintang, Surabaya 60231, IndonesiaDepartment of Chemistry, Faculty of Science and Mathematics, Universitas Negeri Surabaya, Jl. Ketintang, Surabaya 60231, IndonesiaHydroxyapatite is an inorganic material that is commonly used as a re-mineralizing agent. Adding natural ingredients such as green betel leaf can increase the antibacterial properties due to the presence of phenolic compounds, flavonoids, and tannins. This study aims to determine the physical and chemical characteristics of the formulation of hydroxyapatite-betel leaf extract and the antibacterial activity against Streptococcus mutans. To characterize the combination of hydroxyapatite-betel leaf extract, XRD, PSA and FTIR analyses were performed. Particle size analysis showed the smallest results in the variation of betel 0.3 g, which is 690.08 nm. FTIR characterization showed the presence of OH, PO43− and CO32− functional groups from hydroxyapatite and C=O derived from betel leaf extract. In addition, in vitro and in vivo analyses were performed to assess the antibacterial activity of this formulation. The in vitro antibacterial activity test against S. mutans showed strong inhibitory activity. Our finding suggests that the formulation has the potential to be used as a medication or prevention agent for dental caries.https://jurnal.ugm.ac.id/ijc/article/view/77853hydroxyapatitebetel leafstreptococcus mutans |
| spellingShingle | Fitriari Izzatunnisa Muhaimin Sari Edi Cahyaningrum Riska Amelia Lawarti Dina Kartika Maharani Characterization and Antibacterial Activity Assessment of Hydroxyapatite-Betel Leaf Extract Formulation against <i>Streptococcus mutans In Vitro</i> and <i>In Vivo</i> Indonesian Journal of Chemistry hydroxyapatite betel leaf streptococcus mutans |
| title | Characterization and Antibacterial Activity Assessment of Hydroxyapatite-Betel Leaf Extract Formulation against <i>Streptococcus mutans In Vitro</i> and <i>In Vivo</i> |
| title_full | Characterization and Antibacterial Activity Assessment of Hydroxyapatite-Betel Leaf Extract Formulation against <i>Streptococcus mutans In Vitro</i> and <i>In Vivo</i> |
| title_fullStr | Characterization and Antibacterial Activity Assessment of Hydroxyapatite-Betel Leaf Extract Formulation against <i>Streptococcus mutans In Vitro</i> and <i>In Vivo</i> |
| title_full_unstemmed | Characterization and Antibacterial Activity Assessment of Hydroxyapatite-Betel Leaf Extract Formulation against <i>Streptococcus mutans In Vitro</i> and <i>In Vivo</i> |
| title_short | Characterization and Antibacterial Activity Assessment of Hydroxyapatite-Betel Leaf Extract Formulation against <i>Streptococcus mutans In Vitro</i> and <i>In Vivo</i> |
| title_sort | characterization and antibacterial activity assessment of hydroxyapatite betel leaf extract formulation against i streptococcus mutans in vitro i and i in vivo i |
| topic | hydroxyapatite betel leaf streptococcus mutans |
| url | https://jurnal.ugm.ac.id/ijc/article/view/77853 |
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