ACC Deaminase-Positive Halophilic Bacterial Isolates With Multiple Plant Growth-Promoting Traits Improve the Growth and Yield of Chickpea (Cicer arietinum L.) Under Salinity Stress
In this study, a total of 50 halophilic bacterial isolates were screened for 1-aminocyclopropane-1-carboxylate (ACC) deaminase activity, of these six with the highest ACC deaminase activity were selected for an increase in chickpea yield under salinity. The ACC deaminase activity among the isolates...
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Frontiers Media S.A.
2021-11-01
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| Series: | Frontiers in Agronomy |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fagro.2021.681007/full |
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| author | Yalavarthi Nagaraju Mahadevaswamy Nagaraj M. Naik S. B. Gowdar K. Narayanarao K. Satyanarayanarao |
| author_facet | Yalavarthi Nagaraju Mahadevaswamy Nagaraj M. Naik S. B. Gowdar K. Narayanarao K. Satyanarayanarao |
| author_sort | Yalavarthi Nagaraju |
| collection | DOAJ |
| description | In this study, a total of 50 halophilic bacterial isolates were screened for 1-aminocyclopropane-1-carboxylate (ACC) deaminase activity, of these six with the highest ACC deaminase activity were selected for an increase in chickpea yield under salinity. The ACC deaminase activity among the isolates was ranged between 0.12 and 3.56 mM α-KB mg−1 min−1. These six isolates and one reference strain from the Agricultural College, Raichur, were used in the microcosm experiment during the rabi season of 2018. After 60 days of sowing, decreased rhizosphere pH and electrical conductivity (EC) from 8.4 to 7.6 and 4.3 to 3.4 dS m−1, respectively, were reported in chickpea. Among the treatments, Bacillus safensis (B. safensis)-inoculated plants showed a higher number of flowers (71 flowers/plant), pods (49.3 pods/plant), branches (33.3 branches/plant), and enhanced fresh weight (17.2 g/plant) and dry weight (8.1 g/plant). They were corroborated by improved nitrogen and phosphorus absorption of 71.5 and 43.5%, respectively, in B. safensis-treated plants. Based on the microcosm experimental findings, three cultures improving biometric and yield attributes were chosen for the field investigation. The field study was carried out at the Agricultural Research Station, Ganagavathi, during Kharif 2019. The chickpea plants treated with the consortium [B. safensis, Pseudomonas stutzeri, and Staphylococcus xylosus] increased the superoxide dismutase and catalase activity of plants by 258 and 196%, respectively. In addition, an increase in ascorbate peroxidase activity (0.41 μmol of ascorbate oxidized s−1 g−1 fresh weight) in the leaves and proline content was also recorded. The consortium (B. safensis, P. stutzeri, and S. xylosus) significantly increased nutrient uptake (N and P), the number of flowers, number of pods, and yield by 63.26, 39.03, 110, 59.96, and 17.56%, respectively, in chickpeas. Finally, inoculation with a mixture of three isolates is an effective method for increasing chickpea production under osmotic stress. |
| first_indexed | 2024-12-20T22:28:09Z |
| format | Article |
| id | doaj.art-0bd707e5a6ad4804b674c9205171c549 |
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| language | English |
| last_indexed | 2024-12-20T22:28:09Z |
| publishDate | 2021-11-01 |
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| series | Frontiers in Agronomy |
| spelling | doaj.art-0bd707e5a6ad4804b674c9205171c5492022-12-21T19:24:46ZengFrontiers Media S.A.Frontiers in Agronomy2673-32182021-11-01310.3389/fagro.2021.681007681007ACC Deaminase-Positive Halophilic Bacterial Isolates With Multiple Plant Growth-Promoting Traits Improve the Growth and Yield of Chickpea (Cicer arietinum L.) Under Salinity StressYalavarthi Nagaraju0 Mahadevaswamy1Nagaraj M. Naik2S. B. Gowdar3K. Narayanarao4K. Satyanarayanarao5Department of Agricultural Microbiology, University of Agricultural Sciences (UAS), Raichur, IndiaDepartment of Agricultural Microbiology, College of Agriculture, University of Agricultural Sciences (UAS), Raichur, IndiaPesticide Residue and Food Quality Analysis Lab (PRFQAL), University of Agricultural Sciences (UAS), Raichur, IndiaDepartment of Plant Pathology, University of Agricultural Sciences (UAS), Raichur, IndiaDepartment of Soil Science and Agricultural Chemistry, College of Agriculture, University of Agricultural Sciences (UAS), Raichur, IndiaPGS, University of Agricultural Sciences (UAS), Raichur, IndiaIn this study, a total of 50 halophilic bacterial isolates were screened for 1-aminocyclopropane-1-carboxylate (ACC) deaminase activity, of these six with the highest ACC deaminase activity were selected for an increase in chickpea yield under salinity. The ACC deaminase activity among the isolates was ranged between 0.12 and 3.56 mM α-KB mg−1 min−1. These six isolates and one reference strain from the Agricultural College, Raichur, were used in the microcosm experiment during the rabi season of 2018. After 60 days of sowing, decreased rhizosphere pH and electrical conductivity (EC) from 8.4 to 7.6 and 4.3 to 3.4 dS m−1, respectively, were reported in chickpea. Among the treatments, Bacillus safensis (B. safensis)-inoculated plants showed a higher number of flowers (71 flowers/plant), pods (49.3 pods/plant), branches (33.3 branches/plant), and enhanced fresh weight (17.2 g/plant) and dry weight (8.1 g/plant). They were corroborated by improved nitrogen and phosphorus absorption of 71.5 and 43.5%, respectively, in B. safensis-treated plants. Based on the microcosm experimental findings, three cultures improving biometric and yield attributes were chosen for the field investigation. The field study was carried out at the Agricultural Research Station, Ganagavathi, during Kharif 2019. The chickpea plants treated with the consortium [B. safensis, Pseudomonas stutzeri, and Staphylococcus xylosus] increased the superoxide dismutase and catalase activity of plants by 258 and 196%, respectively. In addition, an increase in ascorbate peroxidase activity (0.41 μmol of ascorbate oxidized s−1 g−1 fresh weight) in the leaves and proline content was also recorded. The consortium (B. safensis, P. stutzeri, and S. xylosus) significantly increased nutrient uptake (N and P), the number of flowers, number of pods, and yield by 63.26, 39.03, 110, 59.96, and 17.56%, respectively, in chickpeas. Finally, inoculation with a mixture of three isolates is an effective method for increasing chickpea production under osmotic stress.https://www.frontiersin.org/articles/10.3389/fagro.2021.681007/fullsalinitychickpeamicrohabitatsBacillus safensispHACC deaminase |
| spellingShingle | Yalavarthi Nagaraju Mahadevaswamy Nagaraj M. Naik S. B. Gowdar K. Narayanarao K. Satyanarayanarao ACC Deaminase-Positive Halophilic Bacterial Isolates With Multiple Plant Growth-Promoting Traits Improve the Growth and Yield of Chickpea (Cicer arietinum L.) Under Salinity Stress Frontiers in Agronomy salinity chickpea microhabitats Bacillus safensis pH ACC deaminase |
| title | ACC Deaminase-Positive Halophilic Bacterial Isolates With Multiple Plant Growth-Promoting Traits Improve the Growth and Yield of Chickpea (Cicer arietinum L.) Under Salinity Stress |
| title_full | ACC Deaminase-Positive Halophilic Bacterial Isolates With Multiple Plant Growth-Promoting Traits Improve the Growth and Yield of Chickpea (Cicer arietinum L.) Under Salinity Stress |
| title_fullStr | ACC Deaminase-Positive Halophilic Bacterial Isolates With Multiple Plant Growth-Promoting Traits Improve the Growth and Yield of Chickpea (Cicer arietinum L.) Under Salinity Stress |
| title_full_unstemmed | ACC Deaminase-Positive Halophilic Bacterial Isolates With Multiple Plant Growth-Promoting Traits Improve the Growth and Yield of Chickpea (Cicer arietinum L.) Under Salinity Stress |
| title_short | ACC Deaminase-Positive Halophilic Bacterial Isolates With Multiple Plant Growth-Promoting Traits Improve the Growth and Yield of Chickpea (Cicer arietinum L.) Under Salinity Stress |
| title_sort | acc deaminase positive halophilic bacterial isolates with multiple plant growth promoting traits improve the growth and yield of chickpea cicer arietinum l under salinity stress |
| topic | salinity chickpea microhabitats Bacillus safensis pH ACC deaminase |
| url | https://www.frontiersin.org/articles/10.3389/fagro.2021.681007/full |
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