Identification and characterization of a novel group of natural anti-sense transcripts from RNA1.2 gene locus of human cytomegalovirus
Abstract. Background:. Natural anti-sense transcripts (NATs), which are transcribed from the complementary DNA strand of annotated genes, exert regulatory function of gene expression. Increasing studies recognized anti-sense transcription widespread throughout human cytomegalovirus (HCMV) genome, wh...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Wolters Kluwer
2019-07-01
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| Series: | Chinese Medical Journal |
| Online Access: | http://journals.lww.com/10.1097/CM9.0000000000000299 |
| _version_ | 1818608793131417600 |
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| author | Bing-Yang Liu Yan-Ping Ma Ying Qi Yu-Jing Huang Zhong-Yang Liu Meng-Qing Lu Qiang Ruan Xin Chen |
| author_facet | Bing-Yang Liu Yan-Ping Ma Ying Qi Yu-Jing Huang Zhong-Yang Liu Meng-Qing Lu Qiang Ruan Xin Chen |
| author_sort | Bing-Yang Liu |
| collection | DOAJ |
| description | Abstract. Background:. Natural anti-sense transcripts (NATs), which are transcribed from the complementary DNA strand of annotated genes, exert regulatory function of gene expression. Increasing studies recognized anti-sense transcription widespread throughout human cytomegalovirus (HCMV) genome, whereas the anti-sense transcription of RNA1.2 gene locus has never been investigated. In this study, the transcription of the RNA1.2 anti-sense strand was investigated in clinically isolated HCMV strain.
Methods:. Strand-specific high-through RNA-sequencing (RNA-seq) was performed to find possible anti-sense transcripts (ASTs). For analyzing and visualization of RNA-seq data sets, Integrative Genomics Viewer software was applied. To confirm these possibilities, Northern blotting and rapid amplification of cDNA ends (RACE) were used.
Results:. Transcription of the opposite strand of RNA1.2 gene locus was detected by RNA-sequencing using RNAs extracted from human embryonic lung fibroblasts infected with HCMV clinical isolate HAN. At least three HCMV NATs, named RNA1.2 AST 1, RNA1.2 AST2, and RNA1.2 AST3, were characterized by Northern blotting and RACE analyses. These RNA1.2 ASTs orientated from the complementary strand of RNA1.2 locus during the late phase of HCMV infection. The 5′- and 3′-termini of these transcripts were located within the opposite sequence of the predicted RNA1.2 gene.
Conclusion:. A cluster of novel NATs was transcribed from the opposite sequence of the HCMV RNA1.2 gene region. |
| first_indexed | 2024-12-16T14:48:17Z |
| format | Article |
| id | doaj.art-0bda9e44719b4d98850d9e741813fcae |
| institution | Directory Open Access Journal |
| issn | 0366-6999 2542-5641 |
| language | English |
| last_indexed | 2024-12-16T14:48:17Z |
| publishDate | 2019-07-01 |
| publisher | Wolters Kluwer |
| record_format | Article |
| series | Chinese Medical Journal |
| spelling | doaj.art-0bda9e44719b4d98850d9e741813fcae2022-12-21T22:27:42ZengWolters KluwerChinese Medical Journal0366-69992542-56412019-07-01132131591159810.1097/CM9.0000000000000299201907050-00010Identification and characterization of a novel group of natural anti-sense transcripts from RNA1.2 gene locus of human cytomegalovirusBing-Yang LiuYan-Ping MaYing QiYu-Jing HuangZhong-Yang LiuMeng-Qing LuQiang RuanXin ChenAbstract. Background:. Natural anti-sense transcripts (NATs), which are transcribed from the complementary DNA strand of annotated genes, exert regulatory function of gene expression. Increasing studies recognized anti-sense transcription widespread throughout human cytomegalovirus (HCMV) genome, whereas the anti-sense transcription of RNA1.2 gene locus has never been investigated. In this study, the transcription of the RNA1.2 anti-sense strand was investigated in clinically isolated HCMV strain. Methods:. Strand-specific high-through RNA-sequencing (RNA-seq) was performed to find possible anti-sense transcripts (ASTs). For analyzing and visualization of RNA-seq data sets, Integrative Genomics Viewer software was applied. To confirm these possibilities, Northern blotting and rapid amplification of cDNA ends (RACE) were used. Results:. Transcription of the opposite strand of RNA1.2 gene locus was detected by RNA-sequencing using RNAs extracted from human embryonic lung fibroblasts infected with HCMV clinical isolate HAN. At least three HCMV NATs, named RNA1.2 AST 1, RNA1.2 AST2, and RNA1.2 AST3, were characterized by Northern blotting and RACE analyses. These RNA1.2 ASTs orientated from the complementary strand of RNA1.2 locus during the late phase of HCMV infection. The 5′- and 3′-termini of these transcripts were located within the opposite sequence of the predicted RNA1.2 gene. Conclusion:. A cluster of novel NATs was transcribed from the opposite sequence of the HCMV RNA1.2 gene region.http://journals.lww.com/10.1097/CM9.0000000000000299 |
| spellingShingle | Bing-Yang Liu Yan-Ping Ma Ying Qi Yu-Jing Huang Zhong-Yang Liu Meng-Qing Lu Qiang Ruan Xin Chen Identification and characterization of a novel group of natural anti-sense transcripts from RNA1.2 gene locus of human cytomegalovirus Chinese Medical Journal |
| title | Identification and characterization of a novel group of natural anti-sense transcripts from RNA1.2 gene locus of human cytomegalovirus |
| title_full | Identification and characterization of a novel group of natural anti-sense transcripts from RNA1.2 gene locus of human cytomegalovirus |
| title_fullStr | Identification and characterization of a novel group of natural anti-sense transcripts from RNA1.2 gene locus of human cytomegalovirus |
| title_full_unstemmed | Identification and characterization of a novel group of natural anti-sense transcripts from RNA1.2 gene locus of human cytomegalovirus |
| title_short | Identification and characterization of a novel group of natural anti-sense transcripts from RNA1.2 gene locus of human cytomegalovirus |
| title_sort | identification and characterization of a novel group of natural anti sense transcripts from rna1 2 gene locus of human cytomegalovirus |
| url | http://journals.lww.com/10.1097/CM9.0000000000000299 |
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