<i>Amaranthus cruentus</i> L. Seed Oil Counteracts UVA-Radiation-Induced Inhibition of Collagen Biosynthesis and Wound Healing in Human Skin Fibroblasts
The effect of <i>Amaranthus cruentus</i> L. seed oil (AmO) on collagen biosynthesis and wound healing was studied in cultured human dermal fibroblasts exposed to UVA radiation. It was found that UVA radiation inhibited collagen biosynthesis, prolidase activity, and expression of the β1-i...
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| Format: | Article |
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MDPI AG
2024-01-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/25/2/925 |
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| author | Katarzyna Wolosik Magda Chalecka Jerzy Palka Blanka Mitera Arkadiusz Surazynski |
| author_facet | Katarzyna Wolosik Magda Chalecka Jerzy Palka Blanka Mitera Arkadiusz Surazynski |
| author_sort | Katarzyna Wolosik |
| collection | DOAJ |
| description | The effect of <i>Amaranthus cruentus</i> L. seed oil (AmO) on collagen biosynthesis and wound healing was studied in cultured human dermal fibroblasts exposed to UVA radiation. It was found that UVA radiation inhibited collagen biosynthesis, prolidase activity, and expression of the β1-integrin receptor, and phosphorylated ERK1/2 and TGF-β, while increasing the expression of p38 kinase. The AmO at 0.05–0.15% counteracted the above effects induced by UVA radiation in fibroblasts. UVA radiation also induced the expression and nuclear translocation of the pro-inflammatory NF-κB factor and enhanced the COX-2 expression. AmO effectively suppressed the expression of these pro-inflammatory factors induced by UVA radiation. Expressions of β1 integrin and IGF-I receptors were decreased in the fibroblasts exposed to UVA radiation, while AmO counteracted the effects. Furthermore, AmO stimulated the fibroblast’s migration in a wound healing model, thus facilitating the repair process following exposure of fibroblasts to UVA radiation. These data suggest the potential of AmO to counteract UVA-induced skin damage. |
| first_indexed | 2024-03-08T09:53:30Z |
| format | Article |
| id | doaj.art-0bdc563e352448909875b918aa60edb2 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-08T09:53:30Z |
| publishDate | 2024-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-0bdc563e352448909875b918aa60edb22024-01-29T13:55:37ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672024-01-0125292510.3390/ijms25020925<i>Amaranthus cruentus</i> L. Seed Oil Counteracts UVA-Radiation-Induced Inhibition of Collagen Biosynthesis and Wound Healing in Human Skin FibroblastsKatarzyna Wolosik0Magda Chalecka1Jerzy Palka2Blanka Mitera3Arkadiusz Surazynski4Department of Cosmetology, Medical University of Bialystok, Kilinskiego 1, 15-089 Bialystok, PolandDepartment of Medicinal Chemistry, Medical University of Bialystok, Kilinskiego 1, 15-089 Bialystok, PolandDepartment of Medicinal Chemistry, Medical University of Bialystok, Kilinskiego 1, 15-089 Bialystok, PolandDepartment of Medicinal Chemistry, Medical University of Bialystok, Kilinskiego 1, 15-089 Bialystok, PolandDepartment of Medicinal Chemistry, Medical University of Bialystok, Kilinskiego 1, 15-089 Bialystok, PolandThe effect of <i>Amaranthus cruentus</i> L. seed oil (AmO) on collagen biosynthesis and wound healing was studied in cultured human dermal fibroblasts exposed to UVA radiation. It was found that UVA radiation inhibited collagen biosynthesis, prolidase activity, and expression of the β1-integrin receptor, and phosphorylated ERK1/2 and TGF-β, while increasing the expression of p38 kinase. The AmO at 0.05–0.15% counteracted the above effects induced by UVA radiation in fibroblasts. UVA radiation also induced the expression and nuclear translocation of the pro-inflammatory NF-κB factor and enhanced the COX-2 expression. AmO effectively suppressed the expression of these pro-inflammatory factors induced by UVA radiation. Expressions of β1 integrin and IGF-I receptors were decreased in the fibroblasts exposed to UVA radiation, while AmO counteracted the effects. Furthermore, AmO stimulated the fibroblast’s migration in a wound healing model, thus facilitating the repair process following exposure of fibroblasts to UVA radiation. These data suggest the potential of AmO to counteract UVA-induced skin damage.https://www.mdpi.com/1422-0067/25/2/925UVA radiationdermal fibroblastscollagen biosynthesiswound healing<i>Amaranthus cruentus</i> L. seed oilsun-protective substance |
| spellingShingle | Katarzyna Wolosik Magda Chalecka Jerzy Palka Blanka Mitera Arkadiusz Surazynski <i>Amaranthus cruentus</i> L. Seed Oil Counteracts UVA-Radiation-Induced Inhibition of Collagen Biosynthesis and Wound Healing in Human Skin Fibroblasts International Journal of Molecular Sciences UVA radiation dermal fibroblasts collagen biosynthesis wound healing <i>Amaranthus cruentus</i> L. seed oil sun-protective substance |
| title | <i>Amaranthus cruentus</i> L. Seed Oil Counteracts UVA-Radiation-Induced Inhibition of Collagen Biosynthesis and Wound Healing in Human Skin Fibroblasts |
| title_full | <i>Amaranthus cruentus</i> L. Seed Oil Counteracts UVA-Radiation-Induced Inhibition of Collagen Biosynthesis and Wound Healing in Human Skin Fibroblasts |
| title_fullStr | <i>Amaranthus cruentus</i> L. Seed Oil Counteracts UVA-Radiation-Induced Inhibition of Collagen Biosynthesis and Wound Healing in Human Skin Fibroblasts |
| title_full_unstemmed | <i>Amaranthus cruentus</i> L. Seed Oil Counteracts UVA-Radiation-Induced Inhibition of Collagen Biosynthesis and Wound Healing in Human Skin Fibroblasts |
| title_short | <i>Amaranthus cruentus</i> L. Seed Oil Counteracts UVA-Radiation-Induced Inhibition of Collagen Biosynthesis and Wound Healing in Human Skin Fibroblasts |
| title_sort | i amaranthus cruentus i l seed oil counteracts uva radiation induced inhibition of collagen biosynthesis and wound healing in human skin fibroblasts |
| topic | UVA radiation dermal fibroblasts collagen biosynthesis wound healing <i>Amaranthus cruentus</i> L. seed oil sun-protective substance |
| url | https://www.mdpi.com/1422-0067/25/2/925 |
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