Preparation and Evaluation of Alum Precipitate and Oil Adjuvant Multivalent Vaccines Against Clostridium perfringens
Enterotoxaemia is one of the hazardous diseases of the livestock. In Pakistan prophylaxis failure is due to the vaccination with type D monovalent vaccine. There is a need to develop a cost effective multivalent vaccine against enterotoxaemia using characterized toxinotypes isolated from field. Indi...
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| Format: | Article |
| Language: | English |
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Kafkas University, Faculty of Veterinary Medicine
2021-07-01
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| Series: | Kafkas Universitesi Veteriner Fakültesi Dergisi |
| Subjects: | |
| Online Access: | http://vetdergikafkas.org/uploads/pdf/pdf_KVFD_2813.pdf |
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| author | Madeeha TARIQ Aftab Ahmad ANJUM Ali Ahmad SHEIKH Ali Raza AWAN Muhammad Asad ALI Mian Muhammad Khubaib SATTAR Shahid HUSSAIN Tehreem ALI |
| author_facet | Madeeha TARIQ Aftab Ahmad ANJUM Ali Ahmad SHEIKH Ali Raza AWAN Muhammad Asad ALI Mian Muhammad Khubaib SATTAR Shahid HUSSAIN Tehreem ALI |
| author_sort | Madeeha TARIQ |
| collection | DOAJ |
| description | Enterotoxaemia is one of the hazardous diseases of the livestock. In Pakistan prophylaxis failure is due to the vaccination with type D monovalent vaccine. There is a need to develop a cost effective multivalent vaccine against enterotoxaemia using characterized toxinotypes isolated from field. Indigenously (Punjab, Pakistan) characterized Clostridium perfringens toxinotypes A (MW551947.1), B (MW332247.1) and D (MW332258.1) (n=1 each) were used. These toxinotypes were used to produce higher amount of alpha, beta and epsilon toxin units under culture conditions.
Colony forming units (CFU) of each bacterium was determined through the standard plate count method and 106 CFU/mL bacteria were used for vaccine dose. Monovalent, bivalent and multivalent oil adjuvant and alum precipitate vaccines were prepared. Formulated vaccines were passed the stability, sterility and safety test. Bacterin plus toxoid oil adjuvant vaccine produced higher (868.25±3.54 IU/mL) antibody titer at 28th day post vaccination in rabbits and 100% protection was observed after challenge. Multivalent bacterin plus toxoid oil adjuvant vaccine was
used in field trials. Increased antibody response was detected after 4 months in sheep (1294.81±1.90 IU/mL) and goats (1091.85±2.51 IU/mL). During the experimental and field trials commercial vaccine did not produced higher antibody titer. Multivalent bacterin plus toxoid oil adjuvant vaccine proved as an excellent candidate for vaccination of animals against C. perfringens diseases, and it produced specific and efficient immune response to be used in field. |
| first_indexed | 2024-03-12T10:03:01Z |
| format | Article |
| id | doaj.art-0bdc9262dfa845a5bd646b494c3fda32 |
| institution | Directory Open Access Journal |
| issn | 1300-6045 1309-2251 |
| language | English |
| last_indexed | 2024-03-12T10:03:01Z |
| publishDate | 2021-07-01 |
| publisher | Kafkas University, Faculty of Veterinary Medicine |
| record_format | Article |
| series | Kafkas Universitesi Veteriner Fakültesi Dergisi |
| spelling | doaj.art-0bdc9262dfa845a5bd646b494c3fda322023-09-02T11:26:24ZengKafkas University, Faculty of Veterinary MedicineKafkas Universitesi Veteriner Fakültesi Dergisi1300-60451309-22512021-07-0127447548210.9775/kvfd.2020.25732Preparation and Evaluation of Alum Precipitate and Oil Adjuvant Multivalent Vaccines Against Clostridium perfringensMadeeha TARIQ0https://orcid.org/0000-0001-5235-9419Aftab Ahmad ANJUM1https://orcid.org/0000-0001-8262-8720Ali Ahmad SHEIKH2https://orcid.org/0000-0001-8422-5650Ali Raza AWAN3https://orcid.org/0000-0002-4529-6998Muhammad Asad ALI4https://orcid.org/0000-0003-4038-7054Mian Muhammad Khubaib SATTAR5https://orcid.org/0000-0001-7298-1643Shahid HUSSAIN6https://orcid.org/0000-0001-5138-2051Tehreem ALI7https://orcid.org/0000-0003-0037-3061 Institute of Microbiology, Faculty of Veterinary Science, University of Veterinary and Animal Sciences, 54000, Lahore, PAKISTAN Institute of Microbiology, Faculty of Veterinary Science, University of Veterinary and Animal Sciences, 54000, Lahore, PAKISTANInstitute of Microbiology, Faculty of Veterinary Science, University of Veterinary and Animal Sciences, 54000, Lahore, PAKISTAN Institute of Biochemistry and Biotechnology, Faculty of Biosciences, University of Veterinary and Animal Sciences, 54000, Lahore, PAKISTANInstitute of Microbiology, Faculty of Veterinary Science, University of Veterinary and Animal Sciences, 54000, Lahore, PAKISTANDepartment of Microbiology, Faculty of Veterinary and Animal Sciences, The Islamia University of Bahawalpur, 63100, PAKISTANVaccine Production Section, INTERVAC (PVT) LTD, 18km Lahore Sheikhupura Road, Sheikhupura, PAKISTANInstitute of Microbiology, Faculty of Veterinary Science, University of Veterinary and Animal Sciences, 54000, Lahore, PAKISTANEnterotoxaemia is one of the hazardous diseases of the livestock. In Pakistan prophylaxis failure is due to the vaccination with type D monovalent vaccine. There is a need to develop a cost effective multivalent vaccine against enterotoxaemia using characterized toxinotypes isolated from field. Indigenously (Punjab, Pakistan) characterized Clostridium perfringens toxinotypes A (MW551947.1), B (MW332247.1) and D (MW332258.1) (n=1 each) were used. These toxinotypes were used to produce higher amount of alpha, beta and epsilon toxin units under culture conditions. Colony forming units (CFU) of each bacterium was determined through the standard plate count method and 106 CFU/mL bacteria were used for vaccine dose. Monovalent, bivalent and multivalent oil adjuvant and alum precipitate vaccines were prepared. Formulated vaccines were passed the stability, sterility and safety test. Bacterin plus toxoid oil adjuvant vaccine produced higher (868.25±3.54 IU/mL) antibody titer at 28th day post vaccination in rabbits and 100% protection was observed after challenge. Multivalent bacterin plus toxoid oil adjuvant vaccine was used in field trials. Increased antibody response was detected after 4 months in sheep (1294.81±1.90 IU/mL) and goats (1091.85±2.51 IU/mL). During the experimental and field trials commercial vaccine did not produced higher antibody titer. Multivalent bacterin plus toxoid oil adjuvant vaccine proved as an excellent candidate for vaccination of animals against C. perfringens diseases, and it produced specific and efficient immune response to be used in field.http://vetdergikafkas.org/uploads/pdf/pdf_KVFD_2813.pdfalphabacterinbetaepsilontoxoidvaccine |
| spellingShingle | Madeeha TARIQ Aftab Ahmad ANJUM Ali Ahmad SHEIKH Ali Raza AWAN Muhammad Asad ALI Mian Muhammad Khubaib SATTAR Shahid HUSSAIN Tehreem ALI Preparation and Evaluation of Alum Precipitate and Oil Adjuvant Multivalent Vaccines Against Clostridium perfringens Kafkas Universitesi Veteriner Fakültesi Dergisi alpha bacterin beta epsilon toxoid vaccine |
| title | Preparation and Evaluation of Alum Precipitate and Oil Adjuvant Multivalent Vaccines Against Clostridium perfringens |
| title_full | Preparation and Evaluation of Alum Precipitate and Oil Adjuvant Multivalent Vaccines Against Clostridium perfringens |
| title_fullStr | Preparation and Evaluation of Alum Precipitate and Oil Adjuvant Multivalent Vaccines Against Clostridium perfringens |
| title_full_unstemmed | Preparation and Evaluation of Alum Precipitate and Oil Adjuvant Multivalent Vaccines Against Clostridium perfringens |
| title_short | Preparation and Evaluation of Alum Precipitate and Oil Adjuvant Multivalent Vaccines Against Clostridium perfringens |
| title_sort | preparation and evaluation of alum precipitate and oil adjuvant multivalent vaccines against clostridium perfringens |
| topic | alpha bacterin beta epsilon toxoid vaccine |
| url | http://vetdergikafkas.org/uploads/pdf/pdf_KVFD_2813.pdf |
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