Bio-printing of aligned GelMa-based cell-laden structure for muscle tissue regeneration
Volumetric muscle loss (VML) is associated with a severe loss of muscle tissue that overwhelms the regenerative potential of skeletal muscles. Tissue engineering has shown promise for the treatment of VML injuries, as evidenced by various preclinical trials. The present study describes the fabricati...
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| Format: | Article |
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KeAi Communications Co., Ltd.
2022-02-01
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| Series: | Bioactive Materials |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2452199X21003170 |
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| author | Hanjun Hwangbo Hyeongjin Lee Eun-Ju Jin JaeYoon Lee Yunju Jo Dongryeol Ryu GeunHyung Kim |
| author_facet | Hanjun Hwangbo Hyeongjin Lee Eun-Ju Jin JaeYoon Lee Yunju Jo Dongryeol Ryu GeunHyung Kim |
| author_sort | Hanjun Hwangbo |
| collection | DOAJ |
| description | Volumetric muscle loss (VML) is associated with a severe loss of muscle tissue that overwhelms the regenerative potential of skeletal muscles. Tissue engineering has shown promise for the treatment of VML injuries, as evidenced by various preclinical trials. The present study describes the fabrication of a cell-laden GelMa muscle construct using an in situ crosslinking (ISC) strategy to improve muscle functionality. To obtain optimal biophysical properties of the muscle construct, two UV exposure sources, UV exposure dose, and wall shear stress were evaluated using C2C12 myoblasts. Additionally, the ISC system showed a significantly higher degree of uniaxial alignment and myogenesis compared to the conventional crosslinking strategy (post-crosslinking). To evaluate the in vivo regenerative potential, muscle constructs laden with human adipose stem cells were used. The VML defect group implanted with the bio-printed muscle construct showed significant restoration of functionality and muscular volume. The data presented in this study suggest that stem cell-based therapies combined with the modified bioprinting process could potentially be effective against VML injuries. |
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| format | Article |
| id | doaj.art-0bdd43887c5c4c3c8ef2f9fa36dbfa57 |
| institution | Directory Open Access Journal |
| issn | 2452-199X |
| language | English |
| last_indexed | 2024-04-24T08:24:22Z |
| publishDate | 2022-02-01 |
| publisher | KeAi Communications Co., Ltd. |
| record_format | Article |
| series | Bioactive Materials |
| spelling | doaj.art-0bdd43887c5c4c3c8ef2f9fa36dbfa572024-04-16T23:17:55ZengKeAi Communications Co., Ltd.Bioactive Materials2452-199X2022-02-0185770Bio-printing of aligned GelMa-based cell-laden structure for muscle tissue regenerationHanjun Hwangbo0Hyeongjin Lee1Eun-Ju Jin2JaeYoon Lee3Yunju Jo4Dongryeol Ryu5GeunHyung Kim6Department of Biomechatronics Engineering, College of Biotechnology and Bioengineering, Sungkyunkwan University (SKKU), Suwon, 16419, Republic of KoreaDepartment of Biomechatronics Engineering, College of Biotechnology and Bioengineering, Sungkyunkwan University (SKKU), Suwon, 16419, Republic of KoreaDepartment of Molecular Cell Biology, Sungkyunkwan University School of Medicine (SKKU-SOM), Suwon, 16419, Republic of KoreaDepartment of Biomechatronics Engineering, College of Biotechnology and Bioengineering, Sungkyunkwan University (SKKU), Suwon, 16419, Republic of KoreaDepartment of Molecular Cell Biology, Sungkyunkwan University School of Medicine (SKKU-SOM), Suwon, 16419, Republic of KoreaDepartment of Molecular Cell Biology, Sungkyunkwan University School of Medicine (SKKU-SOM), Suwon, 16419, Republic of Korea; Biomedical Institute for Convergence at SKKU (BICS), Sungkyunkwan University, Suwon, 16419, Republic of Korea; Corresponding author. Department of Molecular Cell Biology, Sungkyunkwan University School of Medicine (SKKU-SOM), Suwon, 16419, Republic of Korea.Department of Biomechatronics Engineering, College of Biotechnology and Bioengineering, Sungkyunkwan University (SKKU), Suwon, 16419, Republic of Korea; Biomedical Institute for Convergence at SKKU (BICS), Sungkyunkwan University, Suwon, 16419, Republic of Korea; Corresponding author. Department of Biomechatronics Engineering, College of Biotechnology and Bioengineering, Sungkyunkwan University (SKKU), Suwon, 16419, Republic of Korea.Volumetric muscle loss (VML) is associated with a severe loss of muscle tissue that overwhelms the regenerative potential of skeletal muscles. Tissue engineering has shown promise for the treatment of VML injuries, as evidenced by various preclinical trials. The present study describes the fabrication of a cell-laden GelMa muscle construct using an in situ crosslinking (ISC) strategy to improve muscle functionality. To obtain optimal biophysical properties of the muscle construct, two UV exposure sources, UV exposure dose, and wall shear stress were evaluated using C2C12 myoblasts. Additionally, the ISC system showed a significantly higher degree of uniaxial alignment and myogenesis compared to the conventional crosslinking strategy (post-crosslinking). To evaluate the in vivo regenerative potential, muscle constructs laden with human adipose stem cells were used. The VML defect group implanted with the bio-printed muscle construct showed significant restoration of functionality and muscular volume. The data presented in this study suggest that stem cell-based therapies combined with the modified bioprinting process could potentially be effective against VML injuries.http://www.sciencedirect.com/science/article/pii/S2452199X21003170GelMa bio-inkin-situ crosslinking3D bioprintingMyogenesis |
| spellingShingle | Hanjun Hwangbo Hyeongjin Lee Eun-Ju Jin JaeYoon Lee Yunju Jo Dongryeol Ryu GeunHyung Kim Bio-printing of aligned GelMa-based cell-laden structure for muscle tissue regeneration Bioactive Materials GelMa bio-ink in-situ crosslinking 3D bioprinting Myogenesis |
| title | Bio-printing of aligned GelMa-based cell-laden structure for muscle tissue regeneration |
| title_full | Bio-printing of aligned GelMa-based cell-laden structure for muscle tissue regeneration |
| title_fullStr | Bio-printing of aligned GelMa-based cell-laden structure for muscle tissue regeneration |
| title_full_unstemmed | Bio-printing of aligned GelMa-based cell-laden structure for muscle tissue regeneration |
| title_short | Bio-printing of aligned GelMa-based cell-laden structure for muscle tissue regeneration |
| title_sort | bio printing of aligned gelma based cell laden structure for muscle tissue regeneration |
| topic | GelMa bio-ink in-situ crosslinking 3D bioprinting Myogenesis |
| url | http://www.sciencedirect.com/science/article/pii/S2452199X21003170 |
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