Promising Insecticidal Properties of Essential Oils from <i>Artemisia aragonensis</i> Lam. and <i>Artemisia negrei</i> L. (Asteraceae) by Targeting Gamma-Aminobutyric Acid and Ryanodine Receptor Proteins: <i>In Vitro</i> and <i>In Silico</i> Approaches
<i>Artemisia negrei</i> (<i>A. negrei</i>) and <i>Artemisia aragonensis</i> (<i>A. aragonensis</i>) are in the family Asteraceae, which has been used in traditional medicine. The use of plant-derived insecticides has become a promising strategy to redu...
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| Format: | Article |
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MDPI AG
2023-05-01
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| Online Access: | https://www.mdpi.com/2297-8739/10/6/329 |
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| author | Khalid Chebbac Zineb Benziane Ouaritini Aimad Allali Burak Tüzün Otmane Zouirech Mohammed Chalkha Abdelfattah El Moussaoui Soufyane Lafraxo Hiba-Allah Nafidi Yousef A. Bin Jardan Mohammed Bourhia Raja Guemmouh |
| author_facet | Khalid Chebbac Zineb Benziane Ouaritini Aimad Allali Burak Tüzün Otmane Zouirech Mohammed Chalkha Abdelfattah El Moussaoui Soufyane Lafraxo Hiba-Allah Nafidi Yousef A. Bin Jardan Mohammed Bourhia Raja Guemmouh |
| author_sort | Khalid Chebbac |
| collection | DOAJ |
| description | <i>Artemisia negrei</i> (<i>A. negrei</i>) and <i>Artemisia aragonensis</i> (<i>A. aragonensis</i>) are in the family Asteraceae, which has been used in traditional medicine. The use of plant-derived insecticides has become a promising strategy to reduce the harmful effects of synthetic insecticides and overcome the bio-resistance of pest insects to insecticides. In this regard, the purpose of the current study was to determine the chemical composition and evaluate insecticidal effects of essential oils (EOs) extracted from <i>A</i>. <i>negrei</i> (EON) and <i>A. aragonensis</i> (EOA). Notably, all chemical constituents present in the EOs were identified through GC-MS analysis, whilst the insecticidal properties against <i>Callosobruchus maculatus</i> Fab. (<i>C. maculatus</i>) were investigated by use of <i>in vitro</i> an <i>in silico</i> approaches. The obtained results showed that both tested EOs present a significant insecticidal effect against <i>C. maculatus</i>, which increased significantly upon the dose used in both contact and inhalation tests. The lethal concentrations (LC<sub>50</sub>) for the inhalation test were found to be 2.1 and 2.97 μL/L, while in the contact test they were 2.08 and 2.74 μL/L of air for EON and EOA, respectively. At 5 μL/L of air, the spawn reduction rate was 88.53 % and 77.41%, while the emergence reduction rate was 94.86% and 81.22% by EON and EOA, respectively. With increasing doses of up to 20 μL/L of air, the reduction in individual emergence reached 100% by the two oils tested after 36 h of treatment. In addition, Molecular docking (MD) simulations supported the <i>in vitro</i> findings and indicated that certain identified components in EOA and EON exhibited stronger hydrogen bonding interactions with the target receptors. Interestingly, the prediction of ADMET properties indicates that the molecules investigated have great pharmacokinetic profiles with no side effects. Taken together, our findings suggest that EOA and EON may exert both potential contact and inhalation insecticidal actions and could be used as an alternative tool for the control of this major insect pest of stored products. |
| first_indexed | 2024-03-11T01:55:38Z |
| format | Article |
| id | doaj.art-0be0f533a6ea44ac83e100c4d3f931e4 |
| institution | Directory Open Access Journal |
| issn | 2297-8739 |
| language | English |
| last_indexed | 2024-03-11T01:55:38Z |
| publishDate | 2023-05-01 |
| publisher | MDPI AG |
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| series | Separations |
| spelling | doaj.art-0be0f533a6ea44ac83e100c4d3f931e42023-11-18T12:36:02ZengMDPI AGSeparations2297-87392023-05-0110632910.3390/separations10060329Promising Insecticidal Properties of Essential Oils from <i>Artemisia aragonensis</i> Lam. and <i>Artemisia negrei</i> L. (Asteraceae) by Targeting Gamma-Aminobutyric Acid and Ryanodine Receptor Proteins: <i>In Vitro</i> and <i>In Silico</i> ApproachesKhalid Chebbac0Zineb Benziane Ouaritini1Aimad Allali2Burak Tüzün3Otmane Zouirech4Mohammed Chalkha5Abdelfattah El Moussaoui6Soufyane Lafraxo7Hiba-Allah Nafidi8Yousef A. Bin Jardan9Mohammed Bourhia10Raja Guemmouh11Laboratory of Biotechnology Conservation and Valorisation of Natural Resources, Faculty of Sciences Dhar El Mahraz, Sidi Mohammed Ben Abdallah University, Fez 30000, MoroccoLaboratory of Natural Substances, Pharmacology, Environment, Modeling, Health and Quality of Life, Faculty of Sciences, Sidi Mohamed Ben Abdellah University, Fez 30000, MoroccoLaboratory of Plant Animal and Agro-Industry Productions, Faculty of Sciences, University of Ibn Tofail, Kenitra 14000, MoroccoDepartment of Plant and Animal Production, Technical Sciences Vocational School of Sivas, Sivas Cumhuriyet University, Sivas 58140, TurkeyLaboratory of Natural Substances, Pharmacology Environment, Modeling, Health and Quality of Life, Faculty of Sciences Dhar El Mahraz, Sidi Mohammed Ben Abdellah University, Fez 30000, MoroccoEngineering Laboratory of Organometallic, Molecular, Materials and Environment, Faculty of Sciences Dhar EL Mahraz, Sidi Mohamed Ben Abdellah University, 30000 Fez, MoroccoLaboratory of Biotechnology, Environment, Agri-Food and Health, Faculty of Sciences Dhar El Mahraz, Sidi Mohammed Ben Abdellah University, Fez 30000, MoroccoLaboratory of Biotechnology, Environment, Agri-Food and Health, Faculty of Sciences Dhar El Mahraz, Sidi Mohammed Ben Abdellah University, Fez 30000, MoroccoDepartment of Food Science, Faculty of Agricultural and Food Sciences, Laval University, 2325 Rue de l’Université, Quebec City, QC G1V 0A6, CanadaDepartment of Pharmaceutics, College of Pharmacy, King Saud University, Riyadh 11451, Saudi ArabiaLaboratory of Chemistry and Biochemistry, Faculty of Medicine and Pharmacy, Ibn Zohr University, Laayoune 70000, MoroccoLaboratory of Biotechnology Conservation and Valorisation of Natural Resources, Faculty of Sciences Dhar El Mahraz, Sidi Mohammed Ben Abdallah University, Fez 30000, Morocco<i>Artemisia negrei</i> (<i>A. negrei</i>) and <i>Artemisia aragonensis</i> (<i>A. aragonensis</i>) are in the family Asteraceae, which has been used in traditional medicine. The use of plant-derived insecticides has become a promising strategy to reduce the harmful effects of synthetic insecticides and overcome the bio-resistance of pest insects to insecticides. In this regard, the purpose of the current study was to determine the chemical composition and evaluate insecticidal effects of essential oils (EOs) extracted from <i>A</i>. <i>negrei</i> (EON) and <i>A. aragonensis</i> (EOA). Notably, all chemical constituents present in the EOs were identified through GC-MS analysis, whilst the insecticidal properties against <i>Callosobruchus maculatus</i> Fab. (<i>C. maculatus</i>) were investigated by use of <i>in vitro</i> an <i>in silico</i> approaches. The obtained results showed that both tested EOs present a significant insecticidal effect against <i>C. maculatus</i>, which increased significantly upon the dose used in both contact and inhalation tests. The lethal concentrations (LC<sub>50</sub>) for the inhalation test were found to be 2.1 and 2.97 μL/L, while in the contact test they were 2.08 and 2.74 μL/L of air for EON and EOA, respectively. At 5 μL/L of air, the spawn reduction rate was 88.53 % and 77.41%, while the emergence reduction rate was 94.86% and 81.22% by EON and EOA, respectively. With increasing doses of up to 20 μL/L of air, the reduction in individual emergence reached 100% by the two oils tested after 36 h of treatment. In addition, Molecular docking (MD) simulations supported the <i>in vitro</i> findings and indicated that certain identified components in EOA and EON exhibited stronger hydrogen bonding interactions with the target receptors. Interestingly, the prediction of ADMET properties indicates that the molecules investigated have great pharmacokinetic profiles with no side effects. Taken together, our findings suggest that EOA and EON may exert both potential contact and inhalation insecticidal actions and could be used as an alternative tool for the control of this major insect pest of stored products.https://www.mdpi.com/2297-8739/10/6/329<i>Artemisia negrei</i><i>Artemisia aragonensis</i>insecticidal activitymolecular dockingADMET properties |
| spellingShingle | Khalid Chebbac Zineb Benziane Ouaritini Aimad Allali Burak Tüzün Otmane Zouirech Mohammed Chalkha Abdelfattah El Moussaoui Soufyane Lafraxo Hiba-Allah Nafidi Yousef A. Bin Jardan Mohammed Bourhia Raja Guemmouh Promising Insecticidal Properties of Essential Oils from <i>Artemisia aragonensis</i> Lam. and <i>Artemisia negrei</i> L. (Asteraceae) by Targeting Gamma-Aminobutyric Acid and Ryanodine Receptor Proteins: <i>In Vitro</i> and <i>In Silico</i> Approaches Separations <i>Artemisia negrei</i> <i>Artemisia aragonensis</i> insecticidal activity molecular docking ADMET properties |
| title | Promising Insecticidal Properties of Essential Oils from <i>Artemisia aragonensis</i> Lam. and <i>Artemisia negrei</i> L. (Asteraceae) by Targeting Gamma-Aminobutyric Acid and Ryanodine Receptor Proteins: <i>In Vitro</i> and <i>In Silico</i> Approaches |
| title_full | Promising Insecticidal Properties of Essential Oils from <i>Artemisia aragonensis</i> Lam. and <i>Artemisia negrei</i> L. (Asteraceae) by Targeting Gamma-Aminobutyric Acid and Ryanodine Receptor Proteins: <i>In Vitro</i> and <i>In Silico</i> Approaches |
| title_fullStr | Promising Insecticidal Properties of Essential Oils from <i>Artemisia aragonensis</i> Lam. and <i>Artemisia negrei</i> L. (Asteraceae) by Targeting Gamma-Aminobutyric Acid and Ryanodine Receptor Proteins: <i>In Vitro</i> and <i>In Silico</i> Approaches |
| title_full_unstemmed | Promising Insecticidal Properties of Essential Oils from <i>Artemisia aragonensis</i> Lam. and <i>Artemisia negrei</i> L. (Asteraceae) by Targeting Gamma-Aminobutyric Acid and Ryanodine Receptor Proteins: <i>In Vitro</i> and <i>In Silico</i> Approaches |
| title_short | Promising Insecticidal Properties of Essential Oils from <i>Artemisia aragonensis</i> Lam. and <i>Artemisia negrei</i> L. (Asteraceae) by Targeting Gamma-Aminobutyric Acid and Ryanodine Receptor Proteins: <i>In Vitro</i> and <i>In Silico</i> Approaches |
| title_sort | promising insecticidal properties of essential oils from i artemisia aragonensis i lam and i artemisia negrei i l asteraceae by targeting gamma aminobutyric acid and ryanodine receptor proteins i in vitro i and i in silico i approaches |
| topic | <i>Artemisia negrei</i> <i>Artemisia aragonensis</i> insecticidal activity molecular docking ADMET properties |
| url | https://www.mdpi.com/2297-8739/10/6/329 |
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