Neuron‐derived extracellular vesicles contain synaptic proteins, promote spine formation, activate TrkB‐mediated signalling and preserve neuronal complexity
Abstract Extracellular vesicles (EVs) play an important role in intercellular communication as carriers of signalling molecules such as bioactive miRNAs, proteins and lipids. EVs are key players in the functioning of the central nervous system (CNS) by influencing synaptic events and modulating reci...
| Main Authors: | , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2023-09-01
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| Series: | Journal of Extracellular Vesicles |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/jev2.12355 |
| _version_ | 1797674109067329536 |
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| author | Julia Solana‐Balaguer Genís Campoy‐Campos Núria Martín‐Flores Leticia Pérez‐Sisqués Laia Sitjà‐Roqueta Melike Kucukerden Ana Gámez‐Valero Albert Coll‐Manzano Eulàlia Martí Esther Pérez‐Navarro Jordi Alberch Jordi Soriano Mercè Masana Cristina Malagelada |
| author_facet | Julia Solana‐Balaguer Genís Campoy‐Campos Núria Martín‐Flores Leticia Pérez‐Sisqués Laia Sitjà‐Roqueta Melike Kucukerden Ana Gámez‐Valero Albert Coll‐Manzano Eulàlia Martí Esther Pérez‐Navarro Jordi Alberch Jordi Soriano Mercè Masana Cristina Malagelada |
| author_sort | Julia Solana‐Balaguer |
| collection | DOAJ |
| description | Abstract Extracellular vesicles (EVs) play an important role in intercellular communication as carriers of signalling molecules such as bioactive miRNAs, proteins and lipids. EVs are key players in the functioning of the central nervous system (CNS) by influencing synaptic events and modulating recipient neurons. However, the specific role of neuron‐to‐neuron communication via EVs is still not well understood. Here, we provide evidence that primary neurons uptake neuron‐derived EVs in the soma, dendrites, and even in the dendritic spines, and carry synaptic proteins. Neuron‐derived EVs increased spine density and promoted the phosphorylation of Akt and ribosomal protein S6 (RPS6), via TrkB‐signalling, without impairing the neuronal network activity. Strikingly, EVs exerted a trophic effect on challenged nutrient‐deprived neurons. Altogether, our results place EVs in the spotlight for synaptic plasticity modulation as well as a possible therapeutic tool to fight neurodegeneration. |
| first_indexed | 2024-03-11T21:55:23Z |
| format | Article |
| id | doaj.art-0be180b1fe9d4b65a4e9bd499c78dd97 |
| institution | Directory Open Access Journal |
| issn | 2001-3078 |
| language | English |
| last_indexed | 2024-03-11T21:55:23Z |
| publishDate | 2023-09-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Extracellular Vesicles |
| spelling | doaj.art-0be180b1fe9d4b65a4e9bd499c78dd972023-09-26T03:14:31ZengWileyJournal of Extracellular Vesicles2001-30782023-09-01129n/an/a10.1002/jev2.12355Neuron‐derived extracellular vesicles contain synaptic proteins, promote spine formation, activate TrkB‐mediated signalling and preserve neuronal complexityJulia Solana‐Balaguer0Genís Campoy‐Campos1Núria Martín‐Flores2Leticia Pérez‐Sisqués3Laia Sitjà‐Roqueta4Melike Kucukerden5Ana Gámez‐Valero6Albert Coll‐Manzano7Eulàlia Martí8Esther Pérez‐Navarro9Jordi Alberch10Jordi Soriano11Mercè Masana12Cristina Malagelada13Departament de Biomedicina, Institut de Neurociències (UBneuro) Universitat de Barcelona BarcelonaSpainDepartament de Biomedicina, Institut de Neurociències (UBneuro) Universitat de Barcelona BarcelonaSpainDepartament de Biomedicina, Institut de Neurociències (UBneuro) Universitat de Barcelona BarcelonaSpainDepartament de Biomedicina, Institut de Neurociències (UBneuro) Universitat de Barcelona BarcelonaSpainDepartament de Biomedicina, Institut de Neurociències (UBneuro) Universitat de Barcelona BarcelonaSpainDepartament de Biomedicina, Institut de Neurociències (UBneuro) Universitat de Barcelona BarcelonaSpainDepartament de Biomedicina, Institut de Neurociències (UBneuro) Universitat de Barcelona BarcelonaSpainDepartament de Biomedicina, Institut de Neurociències (UBneuro) Universitat de Barcelona BarcelonaSpainDepartament de Biomedicina, Institut de Neurociències (UBneuro) Universitat de Barcelona BarcelonaSpainDepartament de Biomedicina, Institut de Neurociències (UBneuro) Universitat de Barcelona BarcelonaSpainDepartament de Biomedicina, Institut de Neurociències (UBneuro) Universitat de Barcelona BarcelonaSpainDepartament de Física de la Matèria Condensada Universitat de Barcelona BarcelonaSpainDepartament de Biomedicina, Institut de Neurociències (UBneuro) Universitat de Barcelona BarcelonaSpainDepartament de Biomedicina, Institut de Neurociències (UBneuro) Universitat de Barcelona BarcelonaSpainAbstract Extracellular vesicles (EVs) play an important role in intercellular communication as carriers of signalling molecules such as bioactive miRNAs, proteins and lipids. EVs are key players in the functioning of the central nervous system (CNS) by influencing synaptic events and modulating recipient neurons. However, the specific role of neuron‐to‐neuron communication via EVs is still not well understood. Here, we provide evidence that primary neurons uptake neuron‐derived EVs in the soma, dendrites, and even in the dendritic spines, and carry synaptic proteins. Neuron‐derived EVs increased spine density and promoted the phosphorylation of Akt and ribosomal protein S6 (RPS6), via TrkB‐signalling, without impairing the neuronal network activity. Strikingly, EVs exerted a trophic effect on challenged nutrient‐deprived neurons. Altogether, our results place EVs in the spotlight for synaptic plasticity modulation as well as a possible therapeutic tool to fight neurodegeneration.https://doi.org/10.1002/jev2.12355extracellular vesicleneuronsynapsesynaptic plasticityTrkB |
| spellingShingle | Julia Solana‐Balaguer Genís Campoy‐Campos Núria Martín‐Flores Leticia Pérez‐Sisqués Laia Sitjà‐Roqueta Melike Kucukerden Ana Gámez‐Valero Albert Coll‐Manzano Eulàlia Martí Esther Pérez‐Navarro Jordi Alberch Jordi Soriano Mercè Masana Cristina Malagelada Neuron‐derived extracellular vesicles contain synaptic proteins, promote spine formation, activate TrkB‐mediated signalling and preserve neuronal complexity Journal of Extracellular Vesicles extracellular vesicle neuron synapse synaptic plasticity TrkB |
| title | Neuron‐derived extracellular vesicles contain synaptic proteins, promote spine formation, activate TrkB‐mediated signalling and preserve neuronal complexity |
| title_full | Neuron‐derived extracellular vesicles contain synaptic proteins, promote spine formation, activate TrkB‐mediated signalling and preserve neuronal complexity |
| title_fullStr | Neuron‐derived extracellular vesicles contain synaptic proteins, promote spine formation, activate TrkB‐mediated signalling and preserve neuronal complexity |
| title_full_unstemmed | Neuron‐derived extracellular vesicles contain synaptic proteins, promote spine formation, activate TrkB‐mediated signalling and preserve neuronal complexity |
| title_short | Neuron‐derived extracellular vesicles contain synaptic proteins, promote spine formation, activate TrkB‐mediated signalling and preserve neuronal complexity |
| title_sort | neuron derived extracellular vesicles contain synaptic proteins promote spine formation activate trkb mediated signalling and preserve neuronal complexity |
| topic | extracellular vesicle neuron synapse synaptic plasticity TrkB |
| url | https://doi.org/10.1002/jev2.12355 |
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