MYB Transcription Factor <i>OsC1<sup>PLSr</sup></i> Involves the Regulation of Purple Leaf Sheath in Rice
Although several regulators associated with purple traits in rice have been identified, the genetic basis of the purple sheath remains unclear. In the present study, F2-1 and F2-2 populations were constructed using purple sheath (H93S) and green sheath (R1173 and YHSM), respectively. In order to ide...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-04-01
|
| Series: | International Journal of Molecular Sciences |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1422-0067/24/7/6655 |
| _version_ | 1797607703710793728 |
|---|---|
| author | Ting Zou Xinyi Wang Tong Sun Huazhen Rong Linxuan Wu Jing Deng Tao Guo Hui Wang Jiafeng Wang Ming Huang |
| author_facet | Ting Zou Xinyi Wang Tong Sun Huazhen Rong Linxuan Wu Jing Deng Tao Guo Hui Wang Jiafeng Wang Ming Huang |
| author_sort | Ting Zou |
| collection | DOAJ |
| description | Although several regulators associated with purple traits in rice have been identified, the genetic basis of the purple sheath remains unclear. In the present study, F2-1 and F2-2 populations were constructed using purple sheath (H93S) and green sheath (R1173 and YHSM), respectively. In order to identify QTL loci in purple sheaths, BSA analyses were performed on the two F2 populations. A crucial QTL for purple sheath was identified, tentatively named qPLSr6, and was located in the 4.61 Mb to 6.03 Mb region of chromosome 6. Combined with expression pattern analysis of candidate genes, <i>LOC_Os06g10350</i> (<i>OsC1<sup>PLSr</sup></i>) was suggested as a candidate gene. The homozygous mutant KO-1 and KO-2 created through CRISPR/Cas9 editing, lost their purple leaf sheath. The RT-PCR revealed that <i>OsC1<sup>PLSr</sup></i>, anthocyanin synthase (ANS), diflavonol-4-reductase (DFR), flavanone-3-hydroxylase (F3H), and flavanone-3′-hydroxylase (F3′H) expression levels were dramatically down-regulated in the mutants. The yeast report system indicated that the 145–272 aa region at the C-terminal of OsC1<sup>PLSr</sup> is a positive transcriptional activation domain. The results indicated that OsC1<sup>PLSr</sup> synthesized anthocyanins by regulating the expression of ANS, DFR, F3H, and F3′H. This study provides new insights into the genetic basis of the purple sheath. |
| first_indexed | 2024-03-11T05:34:31Z |
| format | Article |
| id | doaj.art-0be2021b67184139909de9fa2a9cf881 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-11T05:34:31Z |
| publishDate | 2023-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-0be2021b67184139909de9fa2a9cf8812023-11-17T16:54:12ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-04-01247665510.3390/ijms24076655MYB Transcription Factor <i>OsC1<sup>PLSr</sup></i> Involves the Regulation of Purple Leaf Sheath in RiceTing Zou0Xinyi Wang1Tong Sun2Huazhen Rong3Linxuan Wu4Jing Deng5Tao Guo6Hui Wang7Jiafeng Wang8Ming Huang9Guangdong Provincial Key Laboratory of Plant Molecular Breeding, South China Agricultural University, Guangzhou 510642, ChinaGuangdong Provincial Key Laboratory of Plant Molecular Breeding, South China Agricultural University, Guangzhou 510642, ChinaGuangdong Provincial Key Laboratory of Plant Molecular Breeding, South China Agricultural University, Guangzhou 510642, ChinaGuangdong Provincial Key Laboratory of Plant Molecular Breeding, South China Agricultural University, Guangzhou 510642, ChinaGuangdong Provincial Key Laboratory of Plant Molecular Breeding, South China Agricultural University, Guangzhou 510642, ChinaGuangdong Provincial Key Laboratory of Plant Molecular Breeding, South China Agricultural University, Guangzhou 510642, ChinaGuangdong Provincial Key Laboratory of Plant Molecular Breeding, South China Agricultural University, Guangzhou 510642, ChinaGuangdong Provincial Key Laboratory of Plant Molecular Breeding, South China Agricultural University, Guangzhou 510642, ChinaGuangdong Provincial Key Laboratory of Plant Molecular Breeding, South China Agricultural University, Guangzhou 510642, ChinaGuangdong Provincial Key Laboratory of Plant Molecular Breeding, South China Agricultural University, Guangzhou 510642, ChinaAlthough several regulators associated with purple traits in rice have been identified, the genetic basis of the purple sheath remains unclear. In the present study, F2-1 and F2-2 populations were constructed using purple sheath (H93S) and green sheath (R1173 and YHSM), respectively. In order to identify QTL loci in purple sheaths, BSA analyses were performed on the two F2 populations. A crucial QTL for purple sheath was identified, tentatively named qPLSr6, and was located in the 4.61 Mb to 6.03 Mb region of chromosome 6. Combined with expression pattern analysis of candidate genes, <i>LOC_Os06g10350</i> (<i>OsC1<sup>PLSr</sup></i>) was suggested as a candidate gene. The homozygous mutant KO-1 and KO-2 created through CRISPR/Cas9 editing, lost their purple leaf sheath. The RT-PCR revealed that <i>OsC1<sup>PLSr</sup></i>, anthocyanin synthase (ANS), diflavonol-4-reductase (DFR), flavanone-3-hydroxylase (F3H), and flavanone-3′-hydroxylase (F3′H) expression levels were dramatically down-regulated in the mutants. The yeast report system indicated that the 145–272 aa region at the C-terminal of OsC1<sup>PLSr</sup> is a positive transcriptional activation domain. The results indicated that OsC1<sup>PLSr</sup> synthesized anthocyanins by regulating the expression of ANS, DFR, F3H, and F3′H. This study provides new insights into the genetic basis of the purple sheath.https://www.mdpi.com/1422-0067/24/7/6655ricepurple leaf sheath<i>PLSr</i>transcriptional activityCRISPR/Cas9 |
| spellingShingle | Ting Zou Xinyi Wang Tong Sun Huazhen Rong Linxuan Wu Jing Deng Tao Guo Hui Wang Jiafeng Wang Ming Huang MYB Transcription Factor <i>OsC1<sup>PLSr</sup></i> Involves the Regulation of Purple Leaf Sheath in Rice International Journal of Molecular Sciences rice purple leaf sheath <i>PLSr</i> transcriptional activity CRISPR/Cas9 |
| title | MYB Transcription Factor <i>OsC1<sup>PLSr</sup></i> Involves the Regulation of Purple Leaf Sheath in Rice |
| title_full | MYB Transcription Factor <i>OsC1<sup>PLSr</sup></i> Involves the Regulation of Purple Leaf Sheath in Rice |
| title_fullStr | MYB Transcription Factor <i>OsC1<sup>PLSr</sup></i> Involves the Regulation of Purple Leaf Sheath in Rice |
| title_full_unstemmed | MYB Transcription Factor <i>OsC1<sup>PLSr</sup></i> Involves the Regulation of Purple Leaf Sheath in Rice |
| title_short | MYB Transcription Factor <i>OsC1<sup>PLSr</sup></i> Involves the Regulation of Purple Leaf Sheath in Rice |
| title_sort | myb transcription factor i osc1 sup plsr sup i involves the regulation of purple leaf sheath in rice |
| topic | rice purple leaf sheath <i>PLSr</i> transcriptional activity CRISPR/Cas9 |
| url | https://www.mdpi.com/1422-0067/24/7/6655 |
| work_keys_str_mv | AT tingzou mybtranscriptionfactoriosc1supplsrsupiinvolvestheregulationofpurpleleafsheathinrice AT xinyiwang mybtranscriptionfactoriosc1supplsrsupiinvolvestheregulationofpurpleleafsheathinrice AT tongsun mybtranscriptionfactoriosc1supplsrsupiinvolvestheregulationofpurpleleafsheathinrice AT huazhenrong mybtranscriptionfactoriosc1supplsrsupiinvolvestheregulationofpurpleleafsheathinrice AT linxuanwu mybtranscriptionfactoriosc1supplsrsupiinvolvestheregulationofpurpleleafsheathinrice AT jingdeng mybtranscriptionfactoriosc1supplsrsupiinvolvestheregulationofpurpleleafsheathinrice AT taoguo mybtranscriptionfactoriosc1supplsrsupiinvolvestheregulationofpurpleleafsheathinrice AT huiwang mybtranscriptionfactoriosc1supplsrsupiinvolvestheregulationofpurpleleafsheathinrice AT jiafengwang mybtranscriptionfactoriosc1supplsrsupiinvolvestheregulationofpurpleleafsheathinrice AT minghuang mybtranscriptionfactoriosc1supplsrsupiinvolvestheregulationofpurpleleafsheathinrice |