Investigation of Expression and Methylation Status of ELF5 Gene Promoter in Patients with Breast Cancer
Introduction: Breast cancer is the most prevalent cancer among Iranian women. ELF5 gene as a transcription factor member of the ETS family could play a key role in breast cancer neoplasms, especially basal-like and endocrine-resistant subtypes. The changes in the gene promoter methylation pattern ar...
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| Format: | Article |
| Language: | fas |
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Ilam University of Medical Sciences
2020-10-01
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| Series: | Majallah-i Dānishgāh-i ’Ulūm-i Pizishkī-i Īlām |
| Subjects: | |
| Online Access: | http://sjimu.medilam.ac.ir/article-1-6003-en.html |
| _version_ | 1818564766411522048 |
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| author | Azar Heidarizadi Mahdieh Salimi Hossein Mozdarani |
| author_facet | Azar Heidarizadi Mahdieh Salimi Hossein Mozdarani |
| author_sort | Azar Heidarizadi |
| collection | DOAJ |
| description | Introduction: Breast cancer is the most prevalent cancer among Iranian women. ELF5 gene as a transcription factor member of the ETS family could play a key role in breast cancer neoplasms, especially basal-like and endocrine-resistant subtypes. The changes in the gene promoter methylation pattern are considered proper targets in the therapeutic strategies. This study aimed to investigate the frequency of this epigenetic phenomenon and ELF5 gene expression as well as their association with pathologic and clinical characteristics of Iranian patients suffering from this cancer.
Materials & Methods: In order to investigate the ELF5 promoter methylation, 134 breast tissues were analyzed using methylation-specific PCR method. Moreover, 164 tumoral and 10 normal breast tissues retrieved from breast reduction surgery were assessed using Real-Time RT-PCR to analyze the gene expression.
Ethics code: 52d/4922, 6.10.2016
Findings: The data revealed that about 70% of the breast cancer tumoral specimens showed ELF5 promoter methylated pattern. Furthermore, the down-regulation of ELF5 gene expression was significantly associated with higher cancer stages, being triple-negative, and invasion.
Discussions & Conclusions: The results revealed that an increase in the ELF5 promoter methylation frequency in patients, compared to the control tissues, and its association with poor prognosis indicators may propose the ELF5 promoter methylation as a possible candidate in further studies to confirm the poor prognostic role of this biomarker in breast cancer. |
| first_indexed | 2024-12-14T01:32:53Z |
| format | Article |
| id | doaj.art-0be52da4439c41afb8b223ed87dcf9d7 |
| institution | Directory Open Access Journal |
| issn | 1563-4728 2588-3135 |
| language | fas |
| last_indexed | 2024-12-14T01:32:53Z |
| publishDate | 2020-10-01 |
| publisher | Ilam University of Medical Sciences |
| record_format | Article |
| series | Majallah-i Dānishgāh-i ’Ulūm-i Pizishkī-i Īlām |
| spelling | doaj.art-0be52da4439c41afb8b223ed87dcf9d72022-12-21T23:21:58ZfasIlam University of Medical SciencesMajallah-i Dānishgāh-i ’Ulūm-i Pizishkī-i Īlām1563-47282588-31352020-10-012846678Investigation of Expression and Methylation Status of ELF5 Gene Promoter in Patients with Breast CancerAzar Heidarizadi0Mahdieh Salimi1Hossein Mozdarani2 Dept of Genetics, Fars Science and Research Branch, Islamic Azad University, Marvdasht, Iran Dept of Medical Genetics, Institute of Medical Biotechnology, National Institute of Genetic Engineering and Biotechnology, Tehran, Iran Dept of Medical Genetics, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran Introduction: Breast cancer is the most prevalent cancer among Iranian women. ELF5 gene as a transcription factor member of the ETS family could play a key role in breast cancer neoplasms, especially basal-like and endocrine-resistant subtypes. The changes in the gene promoter methylation pattern are considered proper targets in the therapeutic strategies. This study aimed to investigate the frequency of this epigenetic phenomenon and ELF5 gene expression as well as their association with pathologic and clinical characteristics of Iranian patients suffering from this cancer. Materials & Methods: In order to investigate the ELF5 promoter methylation, 134 breast tissues were analyzed using methylation-specific PCR method. Moreover, 164 tumoral and 10 normal breast tissues retrieved from breast reduction surgery were assessed using Real-Time RT-PCR to analyze the gene expression. Ethics code: 52d/4922, 6.10.2016 Findings: The data revealed that about 70% of the breast cancer tumoral specimens showed ELF5 promoter methylated pattern. Furthermore, the down-regulation of ELF5 gene expression was significantly associated with higher cancer stages, being triple-negative, and invasion. Discussions & Conclusions: The results revealed that an increase in the ELF5 promoter methylation frequency in patients, compared to the control tissues, and its association with poor prognosis indicators may propose the ELF5 promoter methylation as a possible candidate in further studies to confirm the poor prognostic role of this biomarker in breast cancer.http://sjimu.medilam.ac.ir/article-1-6003-en.htmlbreast neoplasmself5epigeneticsgene expressionmethylation |
| spellingShingle | Azar Heidarizadi Mahdieh Salimi Hossein Mozdarani Investigation of Expression and Methylation Status of ELF5 Gene Promoter in Patients with Breast Cancer Majallah-i Dānishgāh-i ’Ulūm-i Pizishkī-i Īlām breast neoplasms elf5 epigenetics gene expression methylation |
| title | Investigation of Expression and Methylation Status
of ELF5 Gene Promoter in Patients
with Breast Cancer |
| title_full | Investigation of Expression and Methylation Status
of ELF5 Gene Promoter in Patients
with Breast Cancer |
| title_fullStr | Investigation of Expression and Methylation Status
of ELF5 Gene Promoter in Patients
with Breast Cancer |
| title_full_unstemmed | Investigation of Expression and Methylation Status
of ELF5 Gene Promoter in Patients
with Breast Cancer |
| title_short | Investigation of Expression and Methylation Status
of ELF5 Gene Promoter in Patients
with Breast Cancer |
| title_sort | investigation of expression and methylation status of elf5 gene promoter in patients with breast cancer |
| topic | breast neoplasms elf5 epigenetics gene expression methylation |
| url | http://sjimu.medilam.ac.ir/article-1-6003-en.html |
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