Identification of a Novel Bioactive Peptide Derived from Frozen Chicken Breast Hydrolysate and the Utilization of Hydrolysates as Biopreservatives
Frozen chicken breast was hydrolyzed by treatment with thermolysin enzyme to obtain a chicken hydrolysate containing bioactive peptides. After that, a peptide was purified from the chicken hydrolysate utilizing a Sep-Pak C18 cartridge and reversed-phase high-performance liquid chromatography (RP-HPL...
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| Format: | Article |
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MDPI AG
2023-09-01
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| Series: | Biology |
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| Online Access: | https://www.mdpi.com/2079-7737/12/9/1218 |
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| author | Mohamed Abdelfattah Maky Takeshi Zendo |
| author_facet | Mohamed Abdelfattah Maky Takeshi Zendo |
| author_sort | Mohamed Abdelfattah Maky |
| collection | DOAJ |
| description | Frozen chicken breast was hydrolyzed by treatment with thermolysin enzyme to obtain a chicken hydrolysate containing bioactive peptides. After that, a peptide was purified from the chicken hydrolysate utilizing a Sep-Pak C18 cartridge and reversed-phase high-performance liquid chromatography (RP-HPLC). The molecular weight of the chicken peptide was 2766.8. Protein sequence analysis showed that the peptide was composed of 25 amino acid residues. The peptide, designated as C25, demonstrated an inhibitory action on the angiotensin-converting enzyme (ACE) with a half maximal inhibitory concentration (IC<sub>50</sub>) value of 1.11 µg/mL. Interestingly, C25 showed antimicrobial activity against multi-drug resistant bacteria <i>Proteus vulgaris</i> F24B and <i>Escherichia coli</i> JM109, both with MIC values of 24 µg/mL. The chicken hydrolysate showed antioxidant activity with an IC<sub>50</sub> value of 348.67 µg/mL. Furthermore, the proliferation of aerobic bacteria and <i>Enterobacteriaceae</i> as well as lipid oxidation were significantly reduced when the chicken hydrolysate was used as a natural preservative during cold storage of chicken breasts. Hydrolysates derived from muscle sources have the potential to be used in formulated food products and to contribute positively to human health. |
| first_indexed | 2024-03-10T23:01:00Z |
| format | Article |
| id | doaj.art-0be7fd42f2c04e7385fa4e0d5d39b966 |
| institution | Directory Open Access Journal |
| issn | 2079-7737 |
| language | English |
| last_indexed | 2024-03-10T23:01:00Z |
| publishDate | 2023-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biology |
| spelling | doaj.art-0be7fd42f2c04e7385fa4e0d5d39b9662023-11-19T09:38:41ZengMDPI AGBiology2079-77372023-09-01129121810.3390/biology12091218Identification of a Novel Bioactive Peptide Derived from Frozen Chicken Breast Hydrolysate and the Utilization of Hydrolysates as BiopreservativesMohamed Abdelfattah Maky0Takeshi Zendo1Laboratory of Microbial Technology, Division of Systems Bioengineering, Department of Bioscience and Biotechnology, Faculty of Agriculture, Graduate School, Kyushu University, 744 Motooka, Nishi-ku, Fukuoka 819-0395, JapanLaboratory of Microbial Technology, Division of Systems Bioengineering, Department of Bioscience and Biotechnology, Faculty of Agriculture, Graduate School, Kyushu University, 744 Motooka, Nishi-ku, Fukuoka 819-0395, JapanFrozen chicken breast was hydrolyzed by treatment with thermolysin enzyme to obtain a chicken hydrolysate containing bioactive peptides. After that, a peptide was purified from the chicken hydrolysate utilizing a Sep-Pak C18 cartridge and reversed-phase high-performance liquid chromatography (RP-HPLC). The molecular weight of the chicken peptide was 2766.8. Protein sequence analysis showed that the peptide was composed of 25 amino acid residues. The peptide, designated as C25, demonstrated an inhibitory action on the angiotensin-converting enzyme (ACE) with a half maximal inhibitory concentration (IC<sub>50</sub>) value of 1.11 µg/mL. Interestingly, C25 showed antimicrobial activity against multi-drug resistant bacteria <i>Proteus vulgaris</i> F24B and <i>Escherichia coli</i> JM109, both with MIC values of 24 µg/mL. The chicken hydrolysate showed antioxidant activity with an IC<sub>50</sub> value of 348.67 µg/mL. Furthermore, the proliferation of aerobic bacteria and <i>Enterobacteriaceae</i> as well as lipid oxidation were significantly reduced when the chicken hydrolysate was used as a natural preservative during cold storage of chicken breasts. Hydrolysates derived from muscle sources have the potential to be used in formulated food products and to contribute positively to human health.https://www.mdpi.com/2079-7737/12/9/1218bioactive peptideschicken breastangiotensin-converting enzymeantioxidantantimicrobial peptidesbiopreservatives |
| spellingShingle | Mohamed Abdelfattah Maky Takeshi Zendo Identification of a Novel Bioactive Peptide Derived from Frozen Chicken Breast Hydrolysate and the Utilization of Hydrolysates as Biopreservatives Biology bioactive peptides chicken breast angiotensin-converting enzyme antioxidant antimicrobial peptides biopreservatives |
| title | Identification of a Novel Bioactive Peptide Derived from Frozen Chicken Breast Hydrolysate and the Utilization of Hydrolysates as Biopreservatives |
| title_full | Identification of a Novel Bioactive Peptide Derived from Frozen Chicken Breast Hydrolysate and the Utilization of Hydrolysates as Biopreservatives |
| title_fullStr | Identification of a Novel Bioactive Peptide Derived from Frozen Chicken Breast Hydrolysate and the Utilization of Hydrolysates as Biopreservatives |
| title_full_unstemmed | Identification of a Novel Bioactive Peptide Derived from Frozen Chicken Breast Hydrolysate and the Utilization of Hydrolysates as Biopreservatives |
| title_short | Identification of a Novel Bioactive Peptide Derived from Frozen Chicken Breast Hydrolysate and the Utilization of Hydrolysates as Biopreservatives |
| title_sort | identification of a novel bioactive peptide derived from frozen chicken breast hydrolysate and the utilization of hydrolysates as biopreservatives |
| topic | bioactive peptides chicken breast angiotensin-converting enzyme antioxidant antimicrobial peptides biopreservatives |
| url | https://www.mdpi.com/2079-7737/12/9/1218 |
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