Antibacterial Activity of TiO<sub>2</sub> Nanoparticles Prepared by One-Step Laser Ablation in Liquid
Laser ablation in liquid was utilized to prepare a TiO<sub>2</sub> NP suspension in in deionized distilled water using Q-switch Nd: YAG laser at various laser energies and ablation times. The samples were characterized using UV–visible absorption spectra obtained with a UV–visible spectr...
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MDPI AG
2021-05-01
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| Series: | Applied Sciences |
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| author | Khawla S. Khashan Ghassan M. Sulaiman Farah A. Abdulameer Salim Albukhaty Mohammed A. Ibrahem Tahani Al-Muhimeed Abeer A. AlObaid |
| author_facet | Khawla S. Khashan Ghassan M. Sulaiman Farah A. Abdulameer Salim Albukhaty Mohammed A. Ibrahem Tahani Al-Muhimeed Abeer A. AlObaid |
| author_sort | Khawla S. Khashan |
| collection | DOAJ |
| description | Laser ablation in liquid was utilized to prepare a TiO<sub>2</sub> NP suspension in in deionized distilled water using Q-switch Nd: YAG laser at various laser energies and ablation times. The samples were characterized using UV–visible absorption spectra obtained with a UV–visible spectrophotometer (UV-Vis,) Fourier transform infrared (FTIR), X-ray diffraction (XRD), and transmission electron microscope (TEM). While, UV-Vis spectra showed the characteristic band-to-band absorption peak of TiO<sub>2</sub> NPs in the UV range. FTIR analysis showed the existence of O-Ti-O bond. XRD patterns indicated the presence of (101) and (112) plane crystalline phases of TiO<sub>2</sub>. TEM images showed a spherical-like structure of TiO<sub>2</sub> NPs with various size distributions depending on the ablation period. It was also found that there is a relationship between laser ablation time and TiO<sub>2</sub> NP size distribution, where longer ablation times led to the smaller size distribution. The antibacterial activity of TiO<sub>2</sub> NPs was evaluated with different species of bacteria such as <i>Escherichia coli</i>, <i>Pseudomonas aeruginosa</i>, <i>Proteus vulgaris</i>, and <i>Staphylococcus aureus</i>, using the liquid approach. The optimum activity of TiO<sub>2</sub> NPs is found to be against <i>E. coli</i> at 1000 μg mL<sup>−1</sup>. Furthermore, adding, TiO<sub>2</sub> NPs (1000 μg mL<sup>−1</sup>) in the presence of amoxicillin has a synergic effect on <i>E. coli</i> and <i>S. aureus</i> growth, as measured by the well diffusion method. However, both <i>E. coli</i> (11.6 ± 0.57mm) and <i>S. aureus</i> (13.3 ± 0.57mm) were inhibited by this process. |
| first_indexed | 2024-03-10T11:16:45Z |
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| issn | 2076-3417 |
| language | English |
| last_indexed | 2024-03-10T11:16:45Z |
| publishDate | 2021-05-01 |
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| series | Applied Sciences |
| spelling | doaj.art-0beb03f161b64f418ad0119b75fed12b2023-11-21T20:20:43ZengMDPI AGApplied Sciences2076-34172021-05-011110462310.3390/app11104623Antibacterial Activity of TiO<sub>2</sub> Nanoparticles Prepared by One-Step Laser Ablation in LiquidKhawla S. Khashan0Ghassan M. Sulaiman1Farah A. Abdulameer2Salim Albukhaty3Mohammed A. Ibrahem4Tahani Al-Muhimeed5Abeer A. AlObaid6Division of Laser Sciences and Technology, Department of Applied Sciences, University of Technology, Baghdad 10066, IraqDivision of Biotechnology, Department of Applied Sciences, University of Technology, Baghdad 10066, IraqCollege of Health and Medical Techniques, Gilgamesh Ahliya University, Baghdad 10022, IraqDepartment of Chemistry, College of Science, University of Misan, Maysan, Amarah 62001, IraqDivision of Laser Sciences and Technology, Department of Applied Sciences, University of Technology, Baghdad 10066, IraqDepartment of Chemistry, College of Science, King Saud University, P.O. Box 22452, Riyadh 11495, Saudi ArabiaDepartment of Chemistry, College of Science, King Saud University, P.O. Box 22452, Riyadh 11495, Saudi ArabiaLaser ablation in liquid was utilized to prepare a TiO<sub>2</sub> NP suspension in in deionized distilled water using Q-switch Nd: YAG laser at various laser energies and ablation times. The samples were characterized using UV–visible absorption spectra obtained with a UV–visible spectrophotometer (UV-Vis,) Fourier transform infrared (FTIR), X-ray diffraction (XRD), and transmission electron microscope (TEM). While, UV-Vis spectra showed the characteristic band-to-band absorption peak of TiO<sub>2</sub> NPs in the UV range. FTIR analysis showed the existence of O-Ti-O bond. XRD patterns indicated the presence of (101) and (112) plane crystalline phases of TiO<sub>2</sub>. TEM images showed a spherical-like structure of TiO<sub>2</sub> NPs with various size distributions depending on the ablation period. It was also found that there is a relationship between laser ablation time and TiO<sub>2</sub> NP size distribution, where longer ablation times led to the smaller size distribution. The antibacterial activity of TiO<sub>2</sub> NPs was evaluated with different species of bacteria such as <i>Escherichia coli</i>, <i>Pseudomonas aeruginosa</i>, <i>Proteus vulgaris</i>, and <i>Staphylococcus aureus</i>, using the liquid approach. The optimum activity of TiO<sub>2</sub> NPs is found to be against <i>E. coli</i> at 1000 μg mL<sup>−1</sup>. Furthermore, adding, TiO<sub>2</sub> NPs (1000 μg mL<sup>−1</sup>) in the presence of amoxicillin has a synergic effect on <i>E. coli</i> and <i>S. aureus</i> growth, as measured by the well diffusion method. However, both <i>E. coli</i> (11.6 ± 0.57mm) and <i>S. aureus</i> (13.3 ± 0.57mm) were inhibited by this process.https://www.mdpi.com/2076-3417/11/10/4623TiO<sub>2</sub> NPslaser ablation in liquidcharacterizationantibacterial activity |
| spellingShingle | Khawla S. Khashan Ghassan M. Sulaiman Farah A. Abdulameer Salim Albukhaty Mohammed A. Ibrahem Tahani Al-Muhimeed Abeer A. AlObaid Antibacterial Activity of TiO<sub>2</sub> Nanoparticles Prepared by One-Step Laser Ablation in Liquid Applied Sciences TiO<sub>2</sub> NPs laser ablation in liquid characterization antibacterial activity |
| title | Antibacterial Activity of TiO<sub>2</sub> Nanoparticles Prepared by One-Step Laser Ablation in Liquid |
| title_full | Antibacterial Activity of TiO<sub>2</sub> Nanoparticles Prepared by One-Step Laser Ablation in Liquid |
| title_fullStr | Antibacterial Activity of TiO<sub>2</sub> Nanoparticles Prepared by One-Step Laser Ablation in Liquid |
| title_full_unstemmed | Antibacterial Activity of TiO<sub>2</sub> Nanoparticles Prepared by One-Step Laser Ablation in Liquid |
| title_short | Antibacterial Activity of TiO<sub>2</sub> Nanoparticles Prepared by One-Step Laser Ablation in Liquid |
| title_sort | antibacterial activity of tio sub 2 sub nanoparticles prepared by one step laser ablation in liquid |
| topic | TiO<sub>2</sub> NPs laser ablation in liquid characterization antibacterial activity |
| url | https://www.mdpi.com/2076-3417/11/10/4623 |
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