Rickettsia sp. and Anaplasma spp. in Haemaphysalis longicornis from Shandong province of China, with evidence of a novel species “Candidatus Anaplasma Shandongensis”
Haemaphysalis longicornis is one of the most dominant and widespread tick species in China. This species mainly infests wild animals and occasionally attacks humans, and has been associated with the transmission of a variety of zoonotic pathogens including spotted fever group Rickettsia (SFGR), seve...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-01-01
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| Series: | Ticks and Tick-Borne Diseases |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1877959X22001844 |
| _version_ | 1827771133445799936 |
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| author | Miao Lu Chao Meng Yilin Li Guangyi Zhou Lin Wang Xiaoyu Xu Na Li Yuqi Ji Junhua Tian Wen Wang Kun Li |
| author_facet | Miao Lu Chao Meng Yilin Li Guangyi Zhou Lin Wang Xiaoyu Xu Na Li Yuqi Ji Junhua Tian Wen Wang Kun Li |
| author_sort | Miao Lu |
| collection | DOAJ |
| description | Haemaphysalis longicornis is one of the most dominant and widespread tick species in China. This species mainly infests wild animals and occasionally attacks humans, and has been associated with the transmission of a variety of zoonotic pathogens including spotted fever group Rickettsia (SFGR), severe fever with thrombocytopenia syndrome virus (SFTSV), Anaplasma phagocytophilum, Babesia spp. and Theileria spp.. Although there are increasing reports of various pathogens associated with H. longicornis, some neglected pathogens in certain areas still need to be studied. In this study, a total of 171 H. longicornis ticks were collected from goats in three locations of Shandong Province, Eastern China (Zibo, Linyi, and Qingdao cities), and subsequently screened for the presence of Rickettsia, Anaplasma, and Ehrlichia bacteria. A total of four bacterial species were identified and characterized. “Candidatus Rickettsia jingxinensis” was detected in one tick specimen from Zibo city. Of 98 ticks from Linyi city, 63.27% (62/98) were tested positive for Anaplasma capra and 5.10% (5/98) were positive for Anaplasma bovis. Interestingly, a novel Anaplasma species was detected and characterized in one tick specimen from Zibo and one other from Linyi, respectively. Genetic and phylogenetic analysis based on the 16S, gltA, groEL, and msp4 genes indicated that it was divergent from all known Anaplasma species but mostly related to A. phagocytophilum and “Cadidatus Anaplasma boleense”. Based on where it was first detected, we named it “Candidatus Anaplasma shandongensis”. |
| first_indexed | 2024-03-11T12:50:18Z |
| format | Article |
| id | doaj.art-0bed8eeaa77a4b7d88a82aa10f68723c |
| institution | Directory Open Access Journal |
| issn | 1877-9603 |
| language | English |
| last_indexed | 2024-03-11T12:50:18Z |
| publishDate | 2023-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Ticks and Tick-Borne Diseases |
| spelling | doaj.art-0bed8eeaa77a4b7d88a82aa10f68723c2023-11-04T04:18:33ZengElsevierTicks and Tick-Borne Diseases1877-96032023-01-01141102082Rickettsia sp. and Anaplasma spp. in Haemaphysalis longicornis from Shandong province of China, with evidence of a novel species “Candidatus Anaplasma Shandongensis”Miao Lu0Chao Meng1Yilin Li2Guangyi Zhou3Lin Wang4Xiaoyu Xu5Na Li6Yuqi Ji7Junhua Tian8Wen Wang9Kun Li10National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, 102206, Changping District, Beijing city, ChinaCollege of life sciences, Shandong First Medical University & Shandong Academy of Medical Sciences, 271016, Taian city, Shandong Province, ChinaCollege of life sciences, Shandong First Medical University & Shandong Academy of Medical Sciences, 271016, Taian city, Shandong Province, ChinaCollege of life sciences, Shandong First Medical University & Shandong Academy of Medical Sciences, 271016, Taian city, Shandong Province, ChinaLaoshan No.3 middle school, 266061, Qingdao city, Shandong Province, ChinaCollege of life sciences, Shandong First Medical University & Shandong Academy of Medical Sciences, 271016, Taian city, Shandong Province, ChinaCollege of life sciences, Shandong First Medical University & Shandong Academy of Medical Sciences, 271016, Taian city, Shandong Province, ChinaCollege of life sciences, Shandong First Medical University & Shandong Academy of Medical Sciences, 271016, Taian city, Shandong Province, ChinaWuhan Center for Disease Control and Prevention, 430024, Wuhan city, Hubei Province, ChinaNational Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, 102206, Changping District, Beijing city, ChinaNational Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, 102206, Changping District, Beijing city, China; Tianjin Key Laboratory of Food and Biotechnology, Tianjin University of Commerce, 300134, Beichen District, Tianjin City, China; Corresponding author at: National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Changping Liuzi 5, 102206, Beijing, ChinaHaemaphysalis longicornis is one of the most dominant and widespread tick species in China. This species mainly infests wild animals and occasionally attacks humans, and has been associated with the transmission of a variety of zoonotic pathogens including spotted fever group Rickettsia (SFGR), severe fever with thrombocytopenia syndrome virus (SFTSV), Anaplasma phagocytophilum, Babesia spp. and Theileria spp.. Although there are increasing reports of various pathogens associated with H. longicornis, some neglected pathogens in certain areas still need to be studied. In this study, a total of 171 H. longicornis ticks were collected from goats in three locations of Shandong Province, Eastern China (Zibo, Linyi, and Qingdao cities), and subsequently screened for the presence of Rickettsia, Anaplasma, and Ehrlichia bacteria. A total of four bacterial species were identified and characterized. “Candidatus Rickettsia jingxinensis” was detected in one tick specimen from Zibo city. Of 98 ticks from Linyi city, 63.27% (62/98) were tested positive for Anaplasma capra and 5.10% (5/98) were positive for Anaplasma bovis. Interestingly, a novel Anaplasma species was detected and characterized in one tick specimen from Zibo and one other from Linyi, respectively. Genetic and phylogenetic analysis based on the 16S, gltA, groEL, and msp4 genes indicated that it was divergent from all known Anaplasma species but mostly related to A. phagocytophilum and “Cadidatus Anaplasma boleense”. Based on where it was first detected, we named it “Candidatus Anaplasma shandongensis”.http://www.sciencedirect.com/science/article/pii/S1877959X22001844Haemaphysalis longicornisShandong ProvinceAnaplasma capraAnaplasma bovis“Candidatus Anaplasma shandongensis” |
| spellingShingle | Miao Lu Chao Meng Yilin Li Guangyi Zhou Lin Wang Xiaoyu Xu Na Li Yuqi Ji Junhua Tian Wen Wang Kun Li Rickettsia sp. and Anaplasma spp. in Haemaphysalis longicornis from Shandong province of China, with evidence of a novel species “Candidatus Anaplasma Shandongensis” Ticks and Tick-Borne Diseases Haemaphysalis longicornis Shandong Province Anaplasma capra Anaplasma bovis “Candidatus Anaplasma shandongensis” |
| title | Rickettsia sp. and Anaplasma spp. in Haemaphysalis longicornis from Shandong province of China, with evidence of a novel species “Candidatus Anaplasma Shandongensis” |
| title_full | Rickettsia sp. and Anaplasma spp. in Haemaphysalis longicornis from Shandong province of China, with evidence of a novel species “Candidatus Anaplasma Shandongensis” |
| title_fullStr | Rickettsia sp. and Anaplasma spp. in Haemaphysalis longicornis from Shandong province of China, with evidence of a novel species “Candidatus Anaplasma Shandongensis” |
| title_full_unstemmed | Rickettsia sp. and Anaplasma spp. in Haemaphysalis longicornis from Shandong province of China, with evidence of a novel species “Candidatus Anaplasma Shandongensis” |
| title_short | Rickettsia sp. and Anaplasma spp. in Haemaphysalis longicornis from Shandong province of China, with evidence of a novel species “Candidatus Anaplasma Shandongensis” |
| title_sort | rickettsia sp and anaplasma spp in haemaphysalis longicornis from shandong province of china with evidence of a novel species candidatus anaplasma shandongensis |
| topic | Haemaphysalis longicornis Shandong Province Anaplasma capra Anaplasma bovis “Candidatus Anaplasma shandongensis” |
| url | http://www.sciencedirect.com/science/article/pii/S1877959X22001844 |
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