Human herpesvirus-8 (HHV-8) antibodies in women from São Paulo, Brazil: association with behavioral factors and Kaposi's sarcoma
BACKGROUND: With the spread of AIDS, many HIV-infected women have been diagnosed with Kaposi's sarcoma (KS), especially in Africa. Since the discovery of a novel herpesvirus as the causative agent of KS (human herpesvirus 8 - HHV-8) several seroepidemiological studies have been conducted to ide...
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| Format: | Article |
| Language: | English |
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Elsevier
2003-01-01
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| Series: | Brazilian Journal of Infectious Diseases |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702003000600007 |
| _version_ | 1818610794450911232 |
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| author | Caterino-de-Araujo Adele Santos-Fortuna Elizabeth de los Carbone Paulo Henrique Lage Cibella Sandra Elisa Lopes Moreira Abdiel Aparecido |
| author_facet | Caterino-de-Araujo Adele Santos-Fortuna Elizabeth de los Carbone Paulo Henrique Lage Cibella Sandra Elisa Lopes Moreira Abdiel Aparecido |
| author_sort | Caterino-de-Araujo Adele |
| collection | DOAJ |
| description | BACKGROUND: With the spread of AIDS, many HIV-infected women have been diagnosed with Kaposi's sarcoma (KS), especially in Africa. Since the discovery of a novel herpesvirus as the causative agent of KS (human herpesvirus 8 - HHV-8) several seroepidemiological studies have been conducted to identify groups at risk for KS. The risk for women in Brazil has not been studied. MATERIALS AND METHODS: We searched for HHV-8 antibodies in sera obtained from a bank made up of samples from 3 groups of individuals: Group I: 163 HIV-1-infected women attended at an ambulatory clinic in 1994; Group II: 108 children born to HIV-1-infected mothers from 1990 to 1992, their antibodies reflected maternal infection, and Group III: 630 HIV-1-seronegative, healthy women. In-house immunofluorescence and Western-Blot assays based on the BCBL-1 cell line were used to detect anti-latent and anti-lytic HHV-8 antibodies. RESULTS: Group I had an overall frequency of antibodies of 8.6%, with a 1.2% frequency of anti-latent antibodies and an 8.0% frequency of anti-lytic antibodies. Similar results were detected in Group II, i.e., no cases with anti-latent antibodies and a 7.4% frequency of anti-lytic antibodies. In contrast, prevalences of 1.1% anti-latent antibodies and 0.3% anti-lytic antibodies were observed in Group III. CONCLUSIONS: The epidemiologic pattern of HHV-8 in women from São Paulo varies according to behavioral factors, with emphasis on the sexual and blood routes of virus transmission/acquisition. Although HHV-8 anti-lytic antibodies were found in HIV-1-infected women, no case of KS was detected. Protective factors against KS are probably related to gender and/or to antiretroviral therapies introduced in Brazil since 1994. |
| first_indexed | 2024-12-16T15:20:06Z |
| format | Article |
| id | doaj.art-0bfced457f1e497ba7452cc4d3a7aa60 |
| institution | Directory Open Access Journal |
| issn | 1413-8670 1678-4391 |
| language | English |
| last_indexed | 2024-12-16T15:20:06Z |
| publishDate | 2003-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Brazilian Journal of Infectious Diseases |
| spelling | doaj.art-0bfced457f1e497ba7452cc4d3a7aa602022-12-21T22:26:39ZengElsevierBrazilian Journal of Infectious Diseases1413-86701678-43912003-01-0176395401Human herpesvirus-8 (HHV-8) antibodies in women from São Paulo, Brazil: association with behavioral factors and Kaposi's sarcomaCaterino-de-Araujo AdeleSantos-Fortuna Elizabeth de losCarbone Paulo Henrique LageCibella Sandra Elisa LopesMoreira Abdiel AparecidoBACKGROUND: With the spread of AIDS, many HIV-infected women have been diagnosed with Kaposi's sarcoma (KS), especially in Africa. Since the discovery of a novel herpesvirus as the causative agent of KS (human herpesvirus 8 - HHV-8) several seroepidemiological studies have been conducted to identify groups at risk for KS. The risk for women in Brazil has not been studied. MATERIALS AND METHODS: We searched for HHV-8 antibodies in sera obtained from a bank made up of samples from 3 groups of individuals: Group I: 163 HIV-1-infected women attended at an ambulatory clinic in 1994; Group II: 108 children born to HIV-1-infected mothers from 1990 to 1992, their antibodies reflected maternal infection, and Group III: 630 HIV-1-seronegative, healthy women. In-house immunofluorescence and Western-Blot assays based on the BCBL-1 cell line were used to detect anti-latent and anti-lytic HHV-8 antibodies. RESULTS: Group I had an overall frequency of antibodies of 8.6%, with a 1.2% frequency of anti-latent antibodies and an 8.0% frequency of anti-lytic antibodies. Similar results were detected in Group II, i.e., no cases with anti-latent antibodies and a 7.4% frequency of anti-lytic antibodies. In contrast, prevalences of 1.1% anti-latent antibodies and 0.3% anti-lytic antibodies were observed in Group III. CONCLUSIONS: The epidemiologic pattern of HHV-8 in women from São Paulo varies according to behavioral factors, with emphasis on the sexual and blood routes of virus transmission/acquisition. Although HHV-8 anti-lytic antibodies were found in HIV-1-infected women, no case of KS was detected. Protective factors against KS are probably related to gender and/or to antiretroviral therapies introduced in Brazil since 1994.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702003000600007Human herpesvirus - 8 (HHV-8) anti-lytic and anti-latent antibodiesHHV-8 infection in womenHHV-8 in HIV-1 infected womenHHV-8 seroprevalenceHHV-8 infectionKaposi's sarcoma (KS) |
| spellingShingle | Caterino-de-Araujo Adele Santos-Fortuna Elizabeth de los Carbone Paulo Henrique Lage Cibella Sandra Elisa Lopes Moreira Abdiel Aparecido Human herpesvirus-8 (HHV-8) antibodies in women from São Paulo, Brazil: association with behavioral factors and Kaposi's sarcoma Brazilian Journal of Infectious Diseases Human herpesvirus - 8 (HHV-8) anti-lytic and anti-latent antibodies HHV-8 infection in women HHV-8 in HIV-1 infected women HHV-8 seroprevalence HHV-8 infection Kaposi's sarcoma (KS) |
| title | Human herpesvirus-8 (HHV-8) antibodies in women from São Paulo, Brazil: association with behavioral factors and Kaposi's sarcoma |
| title_full | Human herpesvirus-8 (HHV-8) antibodies in women from São Paulo, Brazil: association with behavioral factors and Kaposi's sarcoma |
| title_fullStr | Human herpesvirus-8 (HHV-8) antibodies in women from São Paulo, Brazil: association with behavioral factors and Kaposi's sarcoma |
| title_full_unstemmed | Human herpesvirus-8 (HHV-8) antibodies in women from São Paulo, Brazil: association with behavioral factors and Kaposi's sarcoma |
| title_short | Human herpesvirus-8 (HHV-8) antibodies in women from São Paulo, Brazil: association with behavioral factors and Kaposi's sarcoma |
| title_sort | human herpesvirus 8 hhv 8 antibodies in women from sao paulo brazil association with behavioral factors and kaposi s sarcoma |
| topic | Human herpesvirus - 8 (HHV-8) anti-lytic and anti-latent antibodies HHV-8 infection in women HHV-8 in HIV-1 infected women HHV-8 seroprevalence HHV-8 infection Kaposi's sarcoma (KS) |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702003000600007 |
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