Silencing LncRNA SNHG16 suppresses the diabetic inflammatory response by targeting the miR-212-3p/NF-κB signaling pathway
Abstract Background Long noncoding RNAs (LncRNAs) have been identified to play an important role in diabetes. The aim of the present study was to determine the expression and function of small nucleolar RNA host gene 16 (SNHG16) in diabetic inflammation. Methods For the in vitro experiments, quantit...
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| Format: | Article |
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BMC
2023-06-01
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| Series: | Diabetology & Metabolic Syndrome |
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| Online Access: | https://doi.org/10.1186/s13098-023-01070-5 |
| _version_ | 1797806552914067456 |
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| author | Linjuan Huang Shengxi Xiong Hanshuang Liu Ranran Zhang Ying Wu Xiaolei Hu |
| author_facet | Linjuan Huang Shengxi Xiong Hanshuang Liu Ranran Zhang Ying Wu Xiaolei Hu |
| author_sort | Linjuan Huang |
| collection | DOAJ |
| description | Abstract Background Long noncoding RNAs (LncRNAs) have been identified to play an important role in diabetes. The aim of the present study was to determine the expression and function of small nucleolar RNA host gene 16 (SNHG16) in diabetic inflammation. Methods For the in vitro experiments, quantitative real-time PCR (qRT-PCR), Western blotting and immunofluorescence were used to detect LncRNA SNHG16 expression in the high-glucose state. The potential microRNA sponge target of LncRNA SNHG16, miR-212-3p, was detected by dual-luciferase reporter analysis and qRT-PCR. For the in vivo experiments, glucose changes in mice were detected after si-SNHG16 treatment, and SNHG16 and inflammatory factor expression in kidney tissues were detected by qRT-PCR and immunohistochemistry. Results LncRNA SNHG16 was upregulated in diabetic patients, HG-induced THP-1 cells, and diabetic mice. Silencing SNHG16 inhibited the diabetic inflammatory response and the development of diabetic nephropathy. miR-212-3p was found to be directly dependent on LncRNA SNHG16. miR-212-3p could inhibitor P65 phosphorylation in THP-1 cells. The miR-212-3p inhibitor reversed the action of si-SNHG16 in THP-1 cells and induced an inflammatory response in THP-1 cells. LncRNA SNHG16 was also found to be higher in the peripheral blood of diabetic patients than in the normal person. The area under the ROC curve is 0.813. Conclusion These data suggested that silencing LncRNA SNHG16 suppresses diabetic inflammatory responses by competitively binding miR-212-3p to regulate NF-κB. LncRNA SNHG16 can be used as a novel biomarker for patients with type 2 diabetes. |
| first_indexed | 2024-03-13T06:09:00Z |
| format | Article |
| id | doaj.art-0c009f7de56f4f36932ea74581dd4686 |
| institution | Directory Open Access Journal |
| issn | 1758-5996 |
| language | English |
| last_indexed | 2024-03-13T06:09:00Z |
| publishDate | 2023-06-01 |
| publisher | BMC |
| record_format | Article |
| series | Diabetology & Metabolic Syndrome |
| spelling | doaj.art-0c009f7de56f4f36932ea74581dd46862023-06-11T11:21:01ZengBMCDiabetology & Metabolic Syndrome1758-59962023-06-0115111210.1186/s13098-023-01070-5Silencing LncRNA SNHG16 suppresses the diabetic inflammatory response by targeting the miR-212-3p/NF-κB signaling pathwayLinjuan Huang0Shengxi Xiong1Hanshuang Liu2Ranran Zhang3Ying Wu4Xiaolei Hu5The Department of Endocrinology, The First Affiliated Hospital of Bengbu Medical CollegeThe Department of Endocrinology, The First Affiliated Hospital of Bengbu Medical CollegeThe Department of Endocrinology, The First Affiliated Hospital of Bengbu Medical CollegeThe Department of Endocrinology, The First Affiliated Hospital of Bengbu Medical CollegeThe Department of Endocrinology, The First Affiliated Hospital of Bengbu Medical CollegeThe Department of Endocrinology, The First Affiliated Hospital of Bengbu Medical CollegeAbstract Background Long noncoding RNAs (LncRNAs) have been identified to play an important role in diabetes. The aim of the present study was to determine the expression and function of small nucleolar RNA host gene 16 (SNHG16) in diabetic inflammation. Methods For the in vitro experiments, quantitative real-time PCR (qRT-PCR), Western blotting and immunofluorescence were used to detect LncRNA SNHG16 expression in the high-glucose state. The potential microRNA sponge target of LncRNA SNHG16, miR-212-3p, was detected by dual-luciferase reporter analysis and qRT-PCR. For the in vivo experiments, glucose changes in mice were detected after si-SNHG16 treatment, and SNHG16 and inflammatory factor expression in kidney tissues were detected by qRT-PCR and immunohistochemistry. Results LncRNA SNHG16 was upregulated in diabetic patients, HG-induced THP-1 cells, and diabetic mice. Silencing SNHG16 inhibited the diabetic inflammatory response and the development of diabetic nephropathy. miR-212-3p was found to be directly dependent on LncRNA SNHG16. miR-212-3p could inhibitor P65 phosphorylation in THP-1 cells. The miR-212-3p inhibitor reversed the action of si-SNHG16 in THP-1 cells and induced an inflammatory response in THP-1 cells. LncRNA SNHG16 was also found to be higher in the peripheral blood of diabetic patients than in the normal person. The area under the ROC curve is 0.813. Conclusion These data suggested that silencing LncRNA SNHG16 suppresses diabetic inflammatory responses by competitively binding miR-212-3p to regulate NF-κB. LncRNA SNHG16 can be used as a novel biomarker for patients with type 2 diabetes.https://doi.org/10.1186/s13098-023-01070-5LncRNASNHG16miR-212-3pDiabetesInflammation |
| spellingShingle | Linjuan Huang Shengxi Xiong Hanshuang Liu Ranran Zhang Ying Wu Xiaolei Hu Silencing LncRNA SNHG16 suppresses the diabetic inflammatory response by targeting the miR-212-3p/NF-κB signaling pathway Diabetology & Metabolic Syndrome LncRNA SNHG16 miR-212-3p Diabetes Inflammation |
| title | Silencing LncRNA SNHG16 suppresses the diabetic inflammatory response by targeting the miR-212-3p/NF-κB signaling pathway |
| title_full | Silencing LncRNA SNHG16 suppresses the diabetic inflammatory response by targeting the miR-212-3p/NF-κB signaling pathway |
| title_fullStr | Silencing LncRNA SNHG16 suppresses the diabetic inflammatory response by targeting the miR-212-3p/NF-κB signaling pathway |
| title_full_unstemmed | Silencing LncRNA SNHG16 suppresses the diabetic inflammatory response by targeting the miR-212-3p/NF-κB signaling pathway |
| title_short | Silencing LncRNA SNHG16 suppresses the diabetic inflammatory response by targeting the miR-212-3p/NF-κB signaling pathway |
| title_sort | silencing lncrna snhg16 suppresses the diabetic inflammatory response by targeting the mir 212 3p nf κb signaling pathway |
| topic | LncRNA SNHG16 miR-212-3p Diabetes Inflammation |
| url | https://doi.org/10.1186/s13098-023-01070-5 |
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