Lectins Labelled with Digoxin as a Novel Tool to Study Glycoconjugates
In recent years it has become clear that carbohydrate portions of glycoconjugates are performing numerous vital physiological functions in higher organisms. However, since glycobiology is a relatively new science, and carbohydrate structures are highly complex, the continuous development of novel an...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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University of Zagreb Faculty of Food Technology and Biotechnology
2002-01-01
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| Series: | Food Technology and Biotechnology |
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| Online Access: | http://hrcak.srce.hr/file/263203 |
| _version_ | 1827615556546592768 |
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| author | Jerka Dumić Sandra Šupraha Mirna Flögel Gordan Lauc |
| author_facet | Jerka Dumić Sandra Šupraha Mirna Flögel Gordan Lauc |
| author_sort | Jerka Dumić |
| collection | DOAJ |
| description | In recent years it has become clear that carbohydrate portions of glycoconjugates are performing numerous vital physiological functions in higher organisms. However, since glycobiology is a relatively new science, and carbohydrate structures are highly complex, the continuous development of novel analytical techniques is necessary to support the process of understanding the intricate nature of glycoconjugate structure and function. The introduction of digoxin as a novel tag for labelling of lectins that are being used to analyse glycoconjugates in immunoassay-like techniques is described. Lectins labelled with digoxin have significant advantages over biotin- or digoxigenin-labelled lectins and will hopefully prove to be a useful addition to the repertoire of glycobiological tools. |
| first_indexed | 2024-03-09T09:13:31Z |
| format | Article |
| id | doaj.art-0c00d4a7a8944e62bbf149c77f7b16f0 |
| institution | Directory Open Access Journal |
| issn | 1330-9862 1334-2606 |
| language | English |
| last_indexed | 2024-03-09T09:13:31Z |
| publishDate | 2002-01-01 |
| publisher | University of Zagreb Faculty of Food Technology and Biotechnology |
| record_format | Article |
| series | Food Technology and Biotechnology |
| spelling | doaj.art-0c00d4a7a8944e62bbf149c77f7b16f02023-12-02T07:51:09ZengUniversity of Zagreb Faculty of Food Technology and BiotechnologyFood Technology and Biotechnology1330-98621334-26062002-01-01404289292Lectins Labelled with Digoxin as a Novel Tool to Study GlycoconjugatesJerka Dumić0Sandra Šupraha1Mirna Flögel2Gordan Lauc3Department of Biochemistry and Molecular Biology, Faculty of Pharmacy and Biochemistry, University of Zagreb, A. Kova~i}a 1, HR-10000 Zagreb, CroatiaDepartment of Biochemistry and Molecular Biology, Faculty of Pharmacy and Biochemistry, University of Zagreb, A. Kova~i}a 1, HR-10000 Zagreb, CroatiaDepartment of Biochemistry and Molecular Biology, Faculty of Pharmacy and Biochemistry, University of Zagreb, A. Kova~i}a 1, HR-10000 Zagreb, CroatiaDepartment of Biochemistry and Molecular Biology, Faculty of Pharmacy and Biochemistry, University of Zagreb, A. Kova~i}a 1, HR-10000 Zagreb, CroatiaIn recent years it has become clear that carbohydrate portions of glycoconjugates are performing numerous vital physiological functions in higher organisms. However, since glycobiology is a relatively new science, and carbohydrate structures are highly complex, the continuous development of novel analytical techniques is necessary to support the process of understanding the intricate nature of glycoconjugate structure and function. The introduction of digoxin as a novel tag for labelling of lectins that are being used to analyse glycoconjugates in immunoassay-like techniques is described. Lectins labelled with digoxin have significant advantages over biotin- or digoxigenin-labelled lectins and will hopefully prove to be a useful addition to the repertoire of glycobiological tools.http://hrcak.srce.hr/file/263203lectinsglycoconjugatesdigoxinimmunoassay-like techniques |
| spellingShingle | Jerka Dumić Sandra Šupraha Mirna Flögel Gordan Lauc Lectins Labelled with Digoxin as a Novel Tool to Study Glycoconjugates Food Technology and Biotechnology lectins glycoconjugates digoxin immunoassay-like techniques |
| title | Lectins Labelled with Digoxin as a Novel Tool to Study Glycoconjugates |
| title_full | Lectins Labelled with Digoxin as a Novel Tool to Study Glycoconjugates |
| title_fullStr | Lectins Labelled with Digoxin as a Novel Tool to Study Glycoconjugates |
| title_full_unstemmed | Lectins Labelled with Digoxin as a Novel Tool to Study Glycoconjugates |
| title_short | Lectins Labelled with Digoxin as a Novel Tool to Study Glycoconjugates |
| title_sort | lectins labelled with digoxin as a novel tool to study glycoconjugates |
| topic | lectins glycoconjugates digoxin immunoassay-like techniques |
| url | http://hrcak.srce.hr/file/263203 |
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