A novel Penicillium sumatraense isolate reveals an arsenal of degrading enzymes exploitable in algal bio-refinery processes
Abstract Background Microalgae are coming to the spotlight due to their potential applications in a wide number of fields ranging from the biofuel to the pharmaceutical sector. However, several factors such as low productivity, expensive harvesting procedures and difficult metabolite extractability...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2021-09-01
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| Series: | Biotechnology for Biofuels |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s13068-021-02030-9 |
| _version_ | 1818229241142050816 |
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| author | M. Giovannoni I. Larini V. Scafati A. Scortica M. Compri D. Pontiggia G. Zapparoli N. Vitulo M. Benedetti B. Mattei |
| author_facet | M. Giovannoni I. Larini V. Scafati A. Scortica M. Compri D. Pontiggia G. Zapparoli N. Vitulo M. Benedetti B. Mattei |
| author_sort | M. Giovannoni |
| collection | DOAJ |
| description | Abstract Background Microalgae are coming to the spotlight due to their potential applications in a wide number of fields ranging from the biofuel to the pharmaceutical sector. However, several factors such as low productivity, expensive harvesting procedures and difficult metabolite extractability limit their full utilization at industrial scale. Similarly to the successful employment of enzymatic arsenals from lignocellulolytic fungi to convert lignocellulose into fermentable sugars for bioethanol production, specific algalytic formulations could be used to improve the extractability of lipids from microalgae to produce biodiesel. Currently, the research areas related to algivorous organisms, algal saprophytes and the enzymes responsible for the hydrolysis of algal cell wall are still little explored. Results Here, an algal trap method for capturing actively growing microorganisms was successfully used to isolate a filamentous fungus, that was identified by whole-genome sequencing, assembly and annotation as a novel Penicillium sumatraense isolate. The fungus, classified as P. sumatraense AQ67100, was able to assimilate heat-killed Chlorella vulgaris cells by an enzymatic arsenal composed of proteases such as dipeptidyl- and amino-peptidases, β-1,3-glucanases and glycosidases including α- and β-glucosidases, β-glucuronidase, α-mannosidases and β-galactosidases. The treatment of C. vulgaris with the filtrate from P. sumatraense AQ67100 increased the release of chlorophylls and lipids from the algal cells by 42.6 and 48.9%, respectively. Conclusions The improved lipid extractability from C. vulgaris biomass treated with the fungal filtrate highlighted the potential of algal saprophytes in the bioprocessing of microalgae, posing the basis for the sustainable transformation of algal metabolites into biofuel-related compounds. |
| first_indexed | 2024-12-12T10:15:28Z |
| format | Article |
| id | doaj.art-0c00eee1f0b641348e7db64f02526839 |
| institution | Directory Open Access Journal |
| issn | 1754-6834 |
| language | English |
| last_indexed | 2024-12-12T10:15:28Z |
| publishDate | 2021-09-01 |
| publisher | BMC |
| record_format | Article |
| series | Biotechnology for Biofuels |
| spelling | doaj.art-0c00eee1f0b641348e7db64f025268392022-12-22T00:27:41ZengBMCBiotechnology for Biofuels1754-68342021-09-0114112010.1186/s13068-021-02030-9A novel Penicillium sumatraense isolate reveals an arsenal of degrading enzymes exploitable in algal bio-refinery processesM. Giovannoni0I. Larini1V. Scafati2A. Scortica3M. Compri4D. Pontiggia5G. Zapparoli6N. Vitulo7M. Benedetti8B. Mattei9Department of Life, Health and Environmental Sciences, University of L’AquilaDepartment of Biotechnology, University of VeronaDepartment of Life, Health and Environmental Sciences, University of L’AquilaDepartment of Life, Health and Environmental Sciences, University of L’AquilaDepartment of Biotechnology, University of VeronaDepartment of Biology and Biotechnology “Charles Darwin”, Sapienza University of RomeDepartment of Biotechnology, University of VeronaDepartment of Biotechnology, University of VeronaDepartment of Life, Health and Environmental Sciences, University of L’AquilaDepartment of Life, Health and Environmental Sciences, University of L’AquilaAbstract Background Microalgae are coming to the spotlight due to their potential applications in a wide number of fields ranging from the biofuel to the pharmaceutical sector. However, several factors such as low productivity, expensive harvesting procedures and difficult metabolite extractability limit their full utilization at industrial scale. Similarly to the successful employment of enzymatic arsenals from lignocellulolytic fungi to convert lignocellulose into fermentable sugars for bioethanol production, specific algalytic formulations could be used to improve the extractability of lipids from microalgae to produce biodiesel. Currently, the research areas related to algivorous organisms, algal saprophytes and the enzymes responsible for the hydrolysis of algal cell wall are still little explored. Results Here, an algal trap method for capturing actively growing microorganisms was successfully used to isolate a filamentous fungus, that was identified by whole-genome sequencing, assembly and annotation as a novel Penicillium sumatraense isolate. The fungus, classified as P. sumatraense AQ67100, was able to assimilate heat-killed Chlorella vulgaris cells by an enzymatic arsenal composed of proteases such as dipeptidyl- and amino-peptidases, β-1,3-glucanases and glycosidases including α- and β-glucosidases, β-glucuronidase, α-mannosidases and β-galactosidases. The treatment of C. vulgaris with the filtrate from P. sumatraense AQ67100 increased the release of chlorophylls and lipids from the algal cells by 42.6 and 48.9%, respectively. Conclusions The improved lipid extractability from C. vulgaris biomass treated with the fungal filtrate highlighted the potential of algal saprophytes in the bioprocessing of microalgae, posing the basis for the sustainable transformation of algal metabolites into biofuel-related compounds.https://doi.org/10.1186/s13068-021-02030-9Penicillium sumatraenseFunctional genomicsAlgal saprophyteCell wall-degrading enzymesChlorella vulgarisAlgal cell wall |
| spellingShingle | M. Giovannoni I. Larini V. Scafati A. Scortica M. Compri D. Pontiggia G. Zapparoli N. Vitulo M. Benedetti B. Mattei A novel Penicillium sumatraense isolate reveals an arsenal of degrading enzymes exploitable in algal bio-refinery processes Biotechnology for Biofuels Penicillium sumatraense Functional genomics Algal saprophyte Cell wall-degrading enzymes Chlorella vulgaris Algal cell wall |
| title | A novel Penicillium sumatraense isolate reveals an arsenal of degrading enzymes exploitable in algal bio-refinery processes |
| title_full | A novel Penicillium sumatraense isolate reveals an arsenal of degrading enzymes exploitable in algal bio-refinery processes |
| title_fullStr | A novel Penicillium sumatraense isolate reveals an arsenal of degrading enzymes exploitable in algal bio-refinery processes |
| title_full_unstemmed | A novel Penicillium sumatraense isolate reveals an arsenal of degrading enzymes exploitable in algal bio-refinery processes |
| title_short | A novel Penicillium sumatraense isolate reveals an arsenal of degrading enzymes exploitable in algal bio-refinery processes |
| title_sort | novel penicillium sumatraense isolate reveals an arsenal of degrading enzymes exploitable in algal bio refinery processes |
| topic | Penicillium sumatraense Functional genomics Algal saprophyte Cell wall-degrading enzymes Chlorella vulgaris Algal cell wall |
| url | https://doi.org/10.1186/s13068-021-02030-9 |
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