An interventional clinical trial investigating the effects of Spirulina platensis on dental fluorosis and antioxidant system in lambs reared in endemic areas
Abstract This study aimed to evaluate the protective effect of Spirulina platensis primary against dental fluorosis and secondary against oxidative stress in lambs reared in endemic fluorosis areas. Forty-eight lambs aged 5 months were divided into four equal groups (each one including 6 males and 6...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2023-10-01
|
| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-023-44058-x |
| _version_ | 1797576812329435136 |
|---|---|
| author | Abdellatif Rahim Mounia Sibaoueih Adekhalid Essamadi Bouchra El Amiri |
| author_facet | Abdellatif Rahim Mounia Sibaoueih Adekhalid Essamadi Bouchra El Amiri |
| author_sort | Abdellatif Rahim |
| collection | DOAJ |
| description | Abstract This study aimed to evaluate the protective effect of Spirulina platensis primary against dental fluorosis and secondary against oxidative stress in lambs reared in endemic fluorosis areas. Forty-eight lambs aged 5 months were divided into four equal groups (each one including 6 males and 6 females). Groups I and II served as controls belonging respectively to fluorosis-free (Settat) and endemic fluorosis (El Fokra) areas, while the other two Groups III and IV (belonging to El Fokra) received respectively a fixed daily intake of 250 and 500 mg/kg bodyweight (BW) of Spirulina platensis. The experiment was carried out for 13 months until the adult incisors appeared for all animals. According to the Dean’s Fluorosis Index (DFI), 500 mg/kg BW/day of Spirulina platensis (Group IV) protected against dental fluorosis. Moreover, in both male and female lambs, this dose significantly (p < 0.0001) reduced the plasmatic levels of fluoride, proteins, GSH, and MDA compared to the Group II. Furthermore, enzymatic activities of catalase and SOD increased significantly (p < 0.0001) in male and female lambs of the Group IV as compared to Group II. In conclusion, our findings support the potential use of Spirulina platensis as a valuable solution for addressing fluorosis in sheep, warranting further clinical trials. |
| first_indexed | 2024-03-10T21:59:02Z |
| format | Article |
| id | doaj.art-0c0376af9314482b88fbbda5e54c8b92 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-03-10T21:59:02Z |
| publishDate | 2023-10-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-0c0376af9314482b88fbbda5e54c8b922023-11-19T13:00:52ZengNature PortfolioScientific Reports2045-23222023-10-0113111310.1038/s41598-023-44058-xAn interventional clinical trial investigating the effects of Spirulina platensis on dental fluorosis and antioxidant system in lambs reared in endemic areasAbdellatif Rahim0Mounia Sibaoueih1Adekhalid Essamadi2Bouchra El Amiri3Animal Production Unit, Regional Center Agricultural Research of Settat, National Institute for Agricultural Research (INRA)Animal Production Unit, Regional Center Agricultural Research of Settat, National Institute for Agricultural Research (INRA)Laboratory of Biochemistry, Neurosciences, Natural Resources and Environment, Faculty of Sciences and Techniques, Hassan First University of SettatAnimal Production Unit, Regional Center Agricultural Research of Settat, National Institute for Agricultural Research (INRA)Abstract This study aimed to evaluate the protective effect of Spirulina platensis primary against dental fluorosis and secondary against oxidative stress in lambs reared in endemic fluorosis areas. Forty-eight lambs aged 5 months were divided into four equal groups (each one including 6 males and 6 females). Groups I and II served as controls belonging respectively to fluorosis-free (Settat) and endemic fluorosis (El Fokra) areas, while the other two Groups III and IV (belonging to El Fokra) received respectively a fixed daily intake of 250 and 500 mg/kg bodyweight (BW) of Spirulina platensis. The experiment was carried out for 13 months until the adult incisors appeared for all animals. According to the Dean’s Fluorosis Index (DFI), 500 mg/kg BW/day of Spirulina platensis (Group IV) protected against dental fluorosis. Moreover, in both male and female lambs, this dose significantly (p < 0.0001) reduced the plasmatic levels of fluoride, proteins, GSH, and MDA compared to the Group II. Furthermore, enzymatic activities of catalase and SOD increased significantly (p < 0.0001) in male and female lambs of the Group IV as compared to Group II. In conclusion, our findings support the potential use of Spirulina platensis as a valuable solution for addressing fluorosis in sheep, warranting further clinical trials.https://doi.org/10.1038/s41598-023-44058-x |
| spellingShingle | Abdellatif Rahim Mounia Sibaoueih Adekhalid Essamadi Bouchra El Amiri An interventional clinical trial investigating the effects of Spirulina platensis on dental fluorosis and antioxidant system in lambs reared in endemic areas Scientific Reports |
| title | An interventional clinical trial investigating the effects of Spirulina platensis on dental fluorosis and antioxidant system in lambs reared in endemic areas |
| title_full | An interventional clinical trial investigating the effects of Spirulina platensis on dental fluorosis and antioxidant system in lambs reared in endemic areas |
| title_fullStr | An interventional clinical trial investigating the effects of Spirulina platensis on dental fluorosis and antioxidant system in lambs reared in endemic areas |
| title_full_unstemmed | An interventional clinical trial investigating the effects of Spirulina platensis on dental fluorosis and antioxidant system in lambs reared in endemic areas |
| title_short | An interventional clinical trial investigating the effects of Spirulina platensis on dental fluorosis and antioxidant system in lambs reared in endemic areas |
| title_sort | interventional clinical trial investigating the effects of spirulina platensis on dental fluorosis and antioxidant system in lambs reared in endemic areas |
| url | https://doi.org/10.1038/s41598-023-44058-x |
| work_keys_str_mv | AT abdellatifrahim aninterventionalclinicaltrialinvestigatingtheeffectsofspirulinaplatensisondentalfluorosisandantioxidantsysteminlambsrearedinendemicareas AT mouniasibaoueih aninterventionalclinicaltrialinvestigatingtheeffectsofspirulinaplatensisondentalfluorosisandantioxidantsysteminlambsrearedinendemicareas AT adekhalidessamadi aninterventionalclinicaltrialinvestigatingtheeffectsofspirulinaplatensisondentalfluorosisandantioxidantsysteminlambsrearedinendemicareas AT bouchraelamiri aninterventionalclinicaltrialinvestigatingtheeffectsofspirulinaplatensisondentalfluorosisandantioxidantsysteminlambsrearedinendemicareas AT abdellatifrahim interventionalclinicaltrialinvestigatingtheeffectsofspirulinaplatensisondentalfluorosisandantioxidantsysteminlambsrearedinendemicareas AT mouniasibaoueih interventionalclinicaltrialinvestigatingtheeffectsofspirulinaplatensisondentalfluorosisandantioxidantsysteminlambsrearedinendemicareas AT adekhalidessamadi interventionalclinicaltrialinvestigatingtheeffectsofspirulinaplatensisondentalfluorosisandantioxidantsysteminlambsrearedinendemicareas AT bouchraelamiri interventionalclinicaltrialinvestigatingtheeffectsofspirulinaplatensisondentalfluorosisandantioxidantsysteminlambsrearedinendemicareas |