Structure of the mini-RNA-guided endonuclease CRISPR-Cas12j3
The Class 2 family of CRISPR nucleases named Cas12j, which shares only low sequence identity with other CRISPR nucleases was recently identified in the biggiephage clade of phages. Here, the authors present the cryo-EM structure of a functional Cas12j3−crRNA complex in the post-catalytic state and d...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2021-07-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-021-24707-3 |
| _version_ | 1818834663752335360 |
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| author | Arturo Carabias Anders Fuglsang Piero Temperini Tillmann Pape Nicholas Sofos Stefano Stella Simon Erlendsson Guillermo Montoya |
| author_facet | Arturo Carabias Anders Fuglsang Piero Temperini Tillmann Pape Nicholas Sofos Stefano Stella Simon Erlendsson Guillermo Montoya |
| author_sort | Arturo Carabias |
| collection | DOAJ |
| description | The Class 2 family of CRISPR nucleases named Cas12j, which shares only low sequence identity with other CRISPR nucleases was recently identified in the biggiephage clade of phages. Here, the authors present the cryo-EM structure of a functional Cas12j3−crRNA complex in the post-catalytic state and discuss Cas12j3 PAM recognition, hybrid stabilisation and the activation mechanism. |
| first_indexed | 2024-12-19T02:38:24Z |
| format | Article |
| id | doaj.art-0c04ac7b346d45d1bfa7aa86df769b74 |
| institution | Directory Open Access Journal |
| issn | 2041-1723 |
| language | English |
| last_indexed | 2024-12-19T02:38:24Z |
| publishDate | 2021-07-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj.art-0c04ac7b346d45d1bfa7aa86df769b742022-12-21T20:39:16ZengNature PortfolioNature Communications2041-17232021-07-0112111210.1038/s41467-021-24707-3Structure of the mini-RNA-guided endonuclease CRISPR-Cas12j3Arturo Carabias0Anders Fuglsang1Piero Temperini2Tillmann Pape3Nicholas Sofos4Stefano Stella5Simon Erlendsson6Guillermo Montoya7Structural Molecular Biology Group, Novo Nordisk Foundation Centre for Protein Research, Faculty of Health and Medical Sciences University of CopenhagenStructural Molecular Biology Group, Novo Nordisk Foundation Centre for Protein Research, Faculty of Health and Medical Sciences University of CopenhagenStructural Molecular Biology Group, Novo Nordisk Foundation Centre for Protein Research, Faculty of Health and Medical Sciences University of CopenhagenStructural Molecular Biology Group, Novo Nordisk Foundation Centre for Protein Research, Faculty of Health and Medical Sciences University of CopenhagenStructural Molecular Biology Group, Novo Nordisk Foundation Centre for Protein Research, Faculty of Health and Medical Sciences University of CopenhagenStructural Molecular Biology Group, Novo Nordisk Foundation Centre for Protein Research, Faculty of Health and Medical Sciences University of CopenhagenStructural Molecular Biology Group, Novo Nordisk Foundation Centre for Protein Research, Faculty of Health and Medical Sciences University of CopenhagenStructural Molecular Biology Group, Novo Nordisk Foundation Centre for Protein Research, Faculty of Health and Medical Sciences University of CopenhagenThe Class 2 family of CRISPR nucleases named Cas12j, which shares only low sequence identity with other CRISPR nucleases was recently identified in the biggiephage clade of phages. Here, the authors present the cryo-EM structure of a functional Cas12j3−crRNA complex in the post-catalytic state and discuss Cas12j3 PAM recognition, hybrid stabilisation and the activation mechanism.https://doi.org/10.1038/s41467-021-24707-3 |
| spellingShingle | Arturo Carabias Anders Fuglsang Piero Temperini Tillmann Pape Nicholas Sofos Stefano Stella Simon Erlendsson Guillermo Montoya Structure of the mini-RNA-guided endonuclease CRISPR-Cas12j3 Nature Communications |
| title | Structure of the mini-RNA-guided endonuclease CRISPR-Cas12j3 |
| title_full | Structure of the mini-RNA-guided endonuclease CRISPR-Cas12j3 |
| title_fullStr | Structure of the mini-RNA-guided endonuclease CRISPR-Cas12j3 |
| title_full_unstemmed | Structure of the mini-RNA-guided endonuclease CRISPR-Cas12j3 |
| title_short | Structure of the mini-RNA-guided endonuclease CRISPR-Cas12j3 |
| title_sort | structure of the mini rna guided endonuclease crispr cas12j3 |
| url | https://doi.org/10.1038/s41467-021-24707-3 |
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