Molecular Epidemiology of <i>Escherichia coli</i> with Resistance against Third-Generation Cephalosporines Isolated from Deployed German Soldiers—A Retrospective Assessment after Deployments to the African Sahel Region and Other Sites between 2007 and 2016
Colonization and infection with bacteria with acquired antibiotic resistance are among the risks for soldiers on international deployments. Enterobacterales with resistance against third-generation cephalosporines are amongst the most frequently imported microorganisms. To contribute to the scarcely...
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MDPI AG
2022-12-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/10/12/2448 |
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| author | Frederik Pankok Frieder Fuchs Ulrike Loderstädt Martin Kaase Carsten Balczun Simone Scheithauer Hagen Frickmann Ralf Matthias Hagen |
| author_facet | Frederik Pankok Frieder Fuchs Ulrike Loderstädt Martin Kaase Carsten Balczun Simone Scheithauer Hagen Frickmann Ralf Matthias Hagen |
| author_sort | Frederik Pankok |
| collection | DOAJ |
| description | Colonization and infection with bacteria with acquired antibiotic resistance are among the risks for soldiers on international deployments. Enterobacterales with resistance against third-generation cephalosporines are amongst the most frequently imported microorganisms. To contribute to the scarcely available epidemiological knowledge on deployment-associated resistance migration, we assessed the molecular epidemiology of third-generation cephalosporine-resistant <i>Escherichia coli</i> isolated between 2007 and 2016 from German soldiers after deployments, with a particular focus on the African Sahel region. A total of 51 third-generation cephalosporine-resistant <i>E. coli</i> isolated from 51 military returnees from deployment collected during the assessment period between 2007 and 2016 were subjected to short-read next-generation sequencing analysis. Returnees from the Sahel region (Djibouti, Mali, South Sudan, Sudan, Sudan, and Uganda) comprised a proportion of 52.9% (27/51). Repeatedly isolated sequence types according to the Warwick University scheme from returnees from the Sahel region were ST38, ST131, and ST648, confirming previous epidemiological assessments from various sub-Saharan African regions. Locally prevalent resistance genes in isolates from returnees from the Sahel region associated with third-generation resistance were <i>bla</i><sub>CTX-M-15</sub>, <i>bla</i><sub>CTX-M-27</sub>, <i>bla</i><sub>CTX-M-1</sub>, <i>bla</i><sub>TEM-169</sub>, <i>bla</i><sub>CTX-M-14</sub>, <i>bla</i><sub>CTX-M-99</sub>-like, <i>bla</i><sub>CTX-M-125</sub>, <i>bla</i><sub>SHV-12</sub>, and <i>bla</i><sub>DHA-1</sub>, while virulence genes were <i>east1</i>, <i>sat</i>, and <i>tsh</i> in declining order of frequency of occurrence each. In line with phenotypically observed high resistance rates for aminoglycosides and trimethoprim/sulfamethoxazole, multiple associated resistance genes were observed. A similar, slightly more diverse situation was recorded for the other deployment sites. In summary, this assessment provides first next-generation sequencing-based epidemiological data on third-generation cephalosporine-resistant <i>E. coli</i> imported by deployed German soldiers with a particular focus on deployments to the Sahel region, thus serving as a small sentinel. The detected sequence types are well in line with the results from previous epidemiological assessments in sub-Saharan Africa. |
| first_indexed | 2024-03-09T16:03:50Z |
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| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-09T16:03:50Z |
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| series | Microorganisms |
| spelling | doaj.art-0c0c98fe2b9e40128b5b7eb5323cfef32023-11-24T16:49:16ZengMDPI AGMicroorganisms2076-26072022-12-011012244810.3390/microorganisms10122448Molecular Epidemiology of <i>Escherichia coli</i> with Resistance against Third-Generation Cephalosporines Isolated from Deployed German Soldiers—A Retrospective Assessment after Deployments to the African Sahel Region and Other Sites between 2007 and 2016Frederik Pankok0Frieder Fuchs1Ulrike Loderstädt2Martin Kaase3Carsten Balczun4Simone Scheithauer5Hagen Frickmann6Ralf Matthias Hagen7Institute for Infection Control and Infectious Diseases, University Medical Center Göttingen, 37075 Göttingen, GermanyDepartment of Microbiology and Hospital Hygiene, Bundeswehr Central Hospital Koblenz, 56070 Koblenz, GermanyInstitute for Infection Control and Infectious Diseases, University Medical Center Göttingen, 37075 Göttingen, GermanyInstitute for Infection Control and Infectious Diseases, University Medical Center Göttingen, 37075 Göttingen, GermanyDepartment of Microbiology and Hospital Hygiene, Bundeswehr Central Hospital Koblenz, 56070 Koblenz, GermanyInstitute for Infection Control and Infectious Diseases, University Medical Center Göttingen, 37075 Göttingen, GermanyDepartment of Microbiology and Hospital Hygiene, Bundeswehr Hospital Hamburg, 20359 Hamburg, GermanyDepartment of Microbiology and Hospital Hygiene, Bundeswehr Central Hospital Koblenz, 56070 Koblenz, GermanyColonization and infection with bacteria with acquired antibiotic resistance are among the risks for soldiers on international deployments. Enterobacterales with resistance against third-generation cephalosporines are amongst the most frequently imported microorganisms. To contribute to the scarcely available epidemiological knowledge on deployment-associated resistance migration, we assessed the molecular epidemiology of third-generation cephalosporine-resistant <i>Escherichia coli</i> isolated between 2007 and 2016 from German soldiers after deployments, with a particular focus on the African Sahel region. A total of 51 third-generation cephalosporine-resistant <i>E. coli</i> isolated from 51 military returnees from deployment collected during the assessment period between 2007 and 2016 were subjected to short-read next-generation sequencing analysis. Returnees from the Sahel region (Djibouti, Mali, South Sudan, Sudan, Sudan, and Uganda) comprised a proportion of 52.9% (27/51). Repeatedly isolated sequence types according to the Warwick University scheme from returnees from the Sahel region were ST38, ST131, and ST648, confirming previous epidemiological assessments from various sub-Saharan African regions. Locally prevalent resistance genes in isolates from returnees from the Sahel region associated with third-generation resistance were <i>bla</i><sub>CTX-M-15</sub>, <i>bla</i><sub>CTX-M-27</sub>, <i>bla</i><sub>CTX-M-1</sub>, <i>bla</i><sub>TEM-169</sub>, <i>bla</i><sub>CTX-M-14</sub>, <i>bla</i><sub>CTX-M-99</sub>-like, <i>bla</i><sub>CTX-M-125</sub>, <i>bla</i><sub>SHV-12</sub>, and <i>bla</i><sub>DHA-1</sub>, while virulence genes were <i>east1</i>, <i>sat</i>, and <i>tsh</i> in declining order of frequency of occurrence each. In line with phenotypically observed high resistance rates for aminoglycosides and trimethoprim/sulfamethoxazole, multiple associated resistance genes were observed. A similar, slightly more diverse situation was recorded for the other deployment sites. In summary, this assessment provides first next-generation sequencing-based epidemiological data on third-generation cephalosporine-resistant <i>E. coli</i> imported by deployed German soldiers with a particular focus on deployments to the Sahel region, thus serving as a small sentinel. The detected sequence types are well in line with the results from previous epidemiological assessments in sub-Saharan Africa.https://www.mdpi.com/2076-2607/10/12/2448cephalosporine resistanceEnterobacterales<i>Escherichia coli</i>antibiotic resistancesurveillancesub-Saharan Africa |
| spellingShingle | Frederik Pankok Frieder Fuchs Ulrike Loderstädt Martin Kaase Carsten Balczun Simone Scheithauer Hagen Frickmann Ralf Matthias Hagen Molecular Epidemiology of <i>Escherichia coli</i> with Resistance against Third-Generation Cephalosporines Isolated from Deployed German Soldiers—A Retrospective Assessment after Deployments to the African Sahel Region and Other Sites between 2007 and 2016 Microorganisms cephalosporine resistance Enterobacterales <i>Escherichia coli</i> antibiotic resistance surveillance sub-Saharan Africa |
| title | Molecular Epidemiology of <i>Escherichia coli</i> with Resistance against Third-Generation Cephalosporines Isolated from Deployed German Soldiers—A Retrospective Assessment after Deployments to the African Sahel Region and Other Sites between 2007 and 2016 |
| title_full | Molecular Epidemiology of <i>Escherichia coli</i> with Resistance against Third-Generation Cephalosporines Isolated from Deployed German Soldiers—A Retrospective Assessment after Deployments to the African Sahel Region and Other Sites between 2007 and 2016 |
| title_fullStr | Molecular Epidemiology of <i>Escherichia coli</i> with Resistance against Third-Generation Cephalosporines Isolated from Deployed German Soldiers—A Retrospective Assessment after Deployments to the African Sahel Region and Other Sites between 2007 and 2016 |
| title_full_unstemmed | Molecular Epidemiology of <i>Escherichia coli</i> with Resistance against Third-Generation Cephalosporines Isolated from Deployed German Soldiers—A Retrospective Assessment after Deployments to the African Sahel Region and Other Sites between 2007 and 2016 |
| title_short | Molecular Epidemiology of <i>Escherichia coli</i> with Resistance against Third-Generation Cephalosporines Isolated from Deployed German Soldiers—A Retrospective Assessment after Deployments to the African Sahel Region and Other Sites between 2007 and 2016 |
| title_sort | molecular epidemiology of i escherichia coli i with resistance against third generation cephalosporines isolated from deployed german soldiers a retrospective assessment after deployments to the african sahel region and other sites between 2007 and 2016 |
| topic | cephalosporine resistance Enterobacterales <i>Escherichia coli</i> antibiotic resistance surveillance sub-Saharan Africa |
| url | https://www.mdpi.com/2076-2607/10/12/2448 |
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