Unique organizational and functional features of the cytochrome c maturation system in Shewanella oneidensis.
Shewanella are renowned for their ability to respire on a wide range of electron acceptors, which has been partially accredited to the presence of a large number of the c-type cytochromes. In the model species S. oneidensis MR-1, at least 41 genes encode c-type cytochromes that are predicted to be i...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2013-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3769277?pdf=render |
| _version_ | 1811232886825680896 |
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| author | Miao Jin Yaoming Jiang Linlin Sun Jianhua Yin Huihui Fu Genfu Wu Haichun Gao |
| author_facet | Miao Jin Yaoming Jiang Linlin Sun Jianhua Yin Huihui Fu Genfu Wu Haichun Gao |
| author_sort | Miao Jin |
| collection | DOAJ |
| description | Shewanella are renowned for their ability to respire on a wide range of electron acceptors, which has been partially accredited to the presence of a large number of the c-type cytochromes. In the model species S. oneidensis MR-1, at least 41 genes encode c-type cytochromes that are predicted to be intact, thereby likely functional. Previously, in-frame deletion mutants for 36 of these genes were obtained and characterized. In this study, first we completed the construction of an entire set of c-type cytochrome mutants utilizing a newly developed att-based mutagenesis approach, which is more effective and efficient than the approach used previously by circumventing the conventional cloning. Second, we investigated the cytochrome c maturation (Ccm) system in S. oneidensis. There are two loci predicted to encode components of the Ccm system, SO0259-SO0269 and SO0476-SO0478. The former is proven essential for cytochrome c maturation whereas the latter is dispensable. Unlike the single operon organization observed in other γ-proteobacteria, genes at the SO0259-SO0269 locus are uniquely organized into four operons, ccmABCDE, scyA, SO0265, and ccmFGH-SO0269. Functional analysis revealed that the SO0265 gene rather than the scyA and SO0269 genes are relevant to cytochrome c maturation. |
| first_indexed | 2024-04-12T11:10:50Z |
| format | Article |
| id | doaj.art-0c0cbc1402e44cddad27d7fc0761b17c |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-12T11:10:50Z |
| publishDate | 2013-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-0c0cbc1402e44cddad27d7fc0761b17c2022-12-22T03:35:36ZengPublic Library of Science (PLoS)PLoS ONE1932-62032013-01-0189e7561010.1371/journal.pone.0075610Unique organizational and functional features of the cytochrome c maturation system in Shewanella oneidensis.Miao JinYaoming JiangLinlin SunJianhua YinHuihui FuGenfu WuHaichun GaoShewanella are renowned for their ability to respire on a wide range of electron acceptors, which has been partially accredited to the presence of a large number of the c-type cytochromes. In the model species S. oneidensis MR-1, at least 41 genes encode c-type cytochromes that are predicted to be intact, thereby likely functional. Previously, in-frame deletion mutants for 36 of these genes were obtained and characterized. In this study, first we completed the construction of an entire set of c-type cytochrome mutants utilizing a newly developed att-based mutagenesis approach, which is more effective and efficient than the approach used previously by circumventing the conventional cloning. Second, we investigated the cytochrome c maturation (Ccm) system in S. oneidensis. There are two loci predicted to encode components of the Ccm system, SO0259-SO0269 and SO0476-SO0478. The former is proven essential for cytochrome c maturation whereas the latter is dispensable. Unlike the single operon organization observed in other γ-proteobacteria, genes at the SO0259-SO0269 locus are uniquely organized into four operons, ccmABCDE, scyA, SO0265, and ccmFGH-SO0269. Functional analysis revealed that the SO0265 gene rather than the scyA and SO0269 genes are relevant to cytochrome c maturation.http://europepmc.org/articles/PMC3769277?pdf=render |
| spellingShingle | Miao Jin Yaoming Jiang Linlin Sun Jianhua Yin Huihui Fu Genfu Wu Haichun Gao Unique organizational and functional features of the cytochrome c maturation system in Shewanella oneidensis. PLoS ONE |
| title | Unique organizational and functional features of the cytochrome c maturation system in Shewanella oneidensis. |
| title_full | Unique organizational and functional features of the cytochrome c maturation system in Shewanella oneidensis. |
| title_fullStr | Unique organizational and functional features of the cytochrome c maturation system in Shewanella oneidensis. |
| title_full_unstemmed | Unique organizational and functional features of the cytochrome c maturation system in Shewanella oneidensis. |
| title_short | Unique organizational and functional features of the cytochrome c maturation system in Shewanella oneidensis. |
| title_sort | unique organizational and functional features of the cytochrome c maturation system in shewanella oneidensis |
| url | http://europepmc.org/articles/PMC3769277?pdf=render |
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