Helicobacter pylori filtrate impairs spatial learning and memory in rats and increases β-amyloid by enhancing expression of presenilin-2
Helicobacter pylori (H.pylori) infection is related with a high risk of Alzheimer’s Disease (AD), but the intrinsic link between H.pylori infection and AD development is still missing. In the present study, we explored the effect of H.pylori infection on cognitive function and β-amyloid production i...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2014-04-01
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| Series: | Frontiers in Aging Neuroscience |
| Subjects: | |
| Online Access: | http://journal.frontiersin.org/Journal/10.3389/fnagi.2014.00066/full |
| _version_ | 1811277113028771840 |
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| author | Xiu-Lian eWang Xiu-Lian eWang Ji eZeng Jin eFeng Yi-Tao eTian Yu-Jian eLiu Mei eQiu Xiong eYan Yang eYang Yan eXiong Zhi-Hua eZhang Qun eWang Jian-Zhi eWang Rong eLiu |
| author_facet | Xiu-Lian eWang Xiu-Lian eWang Ji eZeng Jin eFeng Yi-Tao eTian Yu-Jian eLiu Mei eQiu Xiong eYan Yang eYang Yan eXiong Zhi-Hua eZhang Qun eWang Jian-Zhi eWang Rong eLiu |
| author_sort | Xiu-Lian eWang |
| collection | DOAJ |
| description | Helicobacter pylori (H.pylori) infection is related with a high risk of Alzheimer’s Disease (AD), but the intrinsic link between H.pylori infection and AD development is still missing. In the present study, we explored the effect of H.pylori infection on cognitive function and β-amyloid production in rats. We found that intraperitoneal injection of H.pylori filtrate induced spatial learning and memory deficit in rats with a simultaneous retarded dendritic spine maturation in hippocampus. Injection of H.pylori filtrate significantly increased Aβ42 both in the hippocampus and cortex, together with an increased level of presenilin-2 (PS-2), one key component of γ-secretase involved in Aβ production. Incubation of H.pylori filtrate with N2a cells which over-express APP also resulted in increased PS-2 expression and Aβ42 overproduction. Injection of Escherichia coli (E.coli) filtrate, another common intestinal bacterium, had no effect on cognitive function in rats and Aβ production in rats and cells. These data suggest a specific effect of H.pylori on cognition and Aβ production. We conclude that soluble surface fractions of H.pylori may promote Aβ42 formation by enhancing the activity of γ-secretase, thus induce cognitive impairment through interrupting the synaptic function. |
| first_indexed | 2024-04-13T00:09:51Z |
| format | Article |
| id | doaj.art-0c0d5f0bc82d421ead67c69eea9fb7c9 |
| institution | Directory Open Access Journal |
| issn | 1663-4365 |
| language | English |
| last_indexed | 2024-04-13T00:09:51Z |
| publishDate | 2014-04-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Aging Neuroscience |
| spelling | doaj.art-0c0d5f0bc82d421ead67c69eea9fb7c92022-12-22T03:11:07ZengFrontiers Media S.A.Frontiers in Aging Neuroscience1663-43652014-04-01610.3389/fnagi.2014.0006685889Helicobacter pylori filtrate impairs spatial learning and memory in rats and increases β-amyloid by enhancing expression of presenilin-2Xiu-Lian eWang0Xiu-Lian eWang1Ji eZeng2Jin eFeng3Yi-Tao eTian4Yu-Jian eLiu5Mei eQiu6Xiong eYan7Yang eYang8Yan eXiong9Zhi-Hua eZhang10Qun eWang11Jian-Zhi eWang12Rong eLiu13Huazhong University of Science and TechnologyHubei University of Chinese MedicineWuhan Pu Ai Hospital, Huazhong University of Science and TechnologyHuazhong University of Science and TechnologyHuazhong University of Science and TechnologyHuazhong University of Science and TechnologyHuazhong University of Science and TechnologyHuazhong University of Science and TechnologyHuazhong University of Science and TechnologyHuazhong University of Science and TechnologyHubei University of Chinese MedicineHuazhong University of Science and TechnologyHuazhong University of Science and TechnologyHuazhong University of Science and TechnologyHelicobacter pylori (H.pylori) infection is related with a high risk of Alzheimer’s Disease (AD), but the intrinsic link between H.pylori infection and AD development is still missing. In the present study, we explored the effect of H.pylori infection on cognitive function and β-amyloid production in rats. We found that intraperitoneal injection of H.pylori filtrate induced spatial learning and memory deficit in rats with a simultaneous retarded dendritic spine maturation in hippocampus. Injection of H.pylori filtrate significantly increased Aβ42 both in the hippocampus and cortex, together with an increased level of presenilin-2 (PS-2), one key component of γ-secretase involved in Aβ production. Incubation of H.pylori filtrate with N2a cells which over-express APP also resulted in increased PS-2 expression and Aβ42 overproduction. Injection of Escherichia coli (E.coli) filtrate, another common intestinal bacterium, had no effect on cognitive function in rats and Aβ production in rats and cells. These data suggest a specific effect of H.pylori on cognition and Aβ production. We conclude that soluble surface fractions of H.pylori may promote Aβ42 formation by enhancing the activity of γ-secretase, thus induce cognitive impairment through interrupting the synaptic function.http://journal.frontiersin.org/Journal/10.3389/fnagi.2014.00066/fullHelicobacter pyloriLearningMemoryPresenilin-2Alzheimer's disease |
| spellingShingle | Xiu-Lian eWang Xiu-Lian eWang Ji eZeng Jin eFeng Yi-Tao eTian Yu-Jian eLiu Mei eQiu Xiong eYan Yang eYang Yan eXiong Zhi-Hua eZhang Qun eWang Jian-Zhi eWang Rong eLiu Helicobacter pylori filtrate impairs spatial learning and memory in rats and increases β-amyloid by enhancing expression of presenilin-2 Frontiers in Aging Neuroscience Helicobacter pylori Learning Memory Presenilin-2 Alzheimer's disease |
| title | Helicobacter pylori filtrate impairs spatial learning and memory in rats and increases β-amyloid by enhancing expression of presenilin-2 |
| title_full | Helicobacter pylori filtrate impairs spatial learning and memory in rats and increases β-amyloid by enhancing expression of presenilin-2 |
| title_fullStr | Helicobacter pylori filtrate impairs spatial learning and memory in rats and increases β-amyloid by enhancing expression of presenilin-2 |
| title_full_unstemmed | Helicobacter pylori filtrate impairs spatial learning and memory in rats and increases β-amyloid by enhancing expression of presenilin-2 |
| title_short | Helicobacter pylori filtrate impairs spatial learning and memory in rats and increases β-amyloid by enhancing expression of presenilin-2 |
| title_sort | helicobacter pylori filtrate impairs spatial learning and memory in rats and increases β amyloid by enhancing expression of presenilin 2 |
| topic | Helicobacter pylori Learning Memory Presenilin-2 Alzheimer's disease |
| url | http://journal.frontiersin.org/Journal/10.3389/fnagi.2014.00066/full |
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