Direct Fungal Production of Ethanol from High-Solids Pulps by the Ethanol-fermenting White-rot Fungus Phlebia sp. MG-60
A white-rot fungus, Phlebia sp. MG-60, was applied to the fermentation of high-solid loadings of unbleached hardwood kraft pulp (UHKP) without the addition of commercial cellulase. From 4.7% UHKP, 19.6 g L-1 ethanol was produced, equivalent to 61.7% of the theoretical maximum. The highest ethanol co...
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| Format: | Article |
| Language: | English |
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North Carolina State University
2014-07-01
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| Series: | BioResources |
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| Online Access: | http://ojs.cnr.ncsu.edu/index.php/BioRes/article/view/BioRes_09_3_5114_Kamei_Fungal_Production_Ethanol_Fungus |
| _version_ | 1811325756715827200 |
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| author | Ichiro Kamei Yoshiyuki Hirota Sadatoshi Meguro |
| author_facet | Ichiro Kamei Yoshiyuki Hirota Sadatoshi Meguro |
| author_sort | Ichiro Kamei |
| collection | DOAJ |
| description | A white-rot fungus, Phlebia sp. MG-60, was applied to the fermentation of high-solid loadings of unbleached hardwood kraft pulp (UHKP) without the addition of commercial cellulase. From 4.7% UHKP, 19.6 g L-1 ethanol was produced, equivalent to 61.7% of the theoretical maximum. The highest ethanol concentration (25.9 g L-1, or 46.7% of the theoretical maximum) was observed in the culture containing 9.1% UHKP. The highest filter paper activity (FPase) was observed in the culture containing 4.7% UHKP, while the production of FPase in the 16.5% UHKP culture was very low. Temporarily removing the silicone plug from Erlenmeyer flasks, which relieved the pressure and allowed a small amount of aeration, improved the yield of ethanol produced from the 9.1% UHKP, which reached as high as 37.3 g L-1. These results indicated that production of cellulase and ensuing saccharification and fermentation by Phlebia sp. MG-60 is affected by water content and benefits from a small amount of aeration. |
| first_indexed | 2024-04-13T14:38:30Z |
| format | Article |
| id | doaj.art-0c0d8b74b195489f9b1579dd58fe8a7f |
| institution | Directory Open Access Journal |
| issn | 1930-2126 1930-2126 |
| language | English |
| last_indexed | 2024-04-13T14:38:30Z |
| publishDate | 2014-07-01 |
| publisher | North Carolina State University |
| record_format | Article |
| series | BioResources |
| spelling | doaj.art-0c0d8b74b195489f9b1579dd58fe8a7f2022-12-22T02:42:57ZengNorth Carolina State UniversityBioResources1930-21261930-21262014-07-01935114512410.15376/biores.9.3.5114-5124Direct Fungal Production of Ethanol from High-Solids Pulps by the Ethanol-fermenting White-rot Fungus Phlebia sp. MG-60Ichiro Kamei0Yoshiyuki Hirota1Sadatoshi Meguro2University of MiyazakiUniversity of MiyazakiUniversity of MiyazakiA white-rot fungus, Phlebia sp. MG-60, was applied to the fermentation of high-solid loadings of unbleached hardwood kraft pulp (UHKP) without the addition of commercial cellulase. From 4.7% UHKP, 19.6 g L-1 ethanol was produced, equivalent to 61.7% of the theoretical maximum. The highest ethanol concentration (25.9 g L-1, or 46.7% of the theoretical maximum) was observed in the culture containing 9.1% UHKP. The highest filter paper activity (FPase) was observed in the culture containing 4.7% UHKP, while the production of FPase in the 16.5% UHKP culture was very low. Temporarily removing the silicone plug from Erlenmeyer flasks, which relieved the pressure and allowed a small amount of aeration, improved the yield of ethanol produced from the 9.1% UHKP, which reached as high as 37.3 g L-1. These results indicated that production of cellulase and ensuing saccharification and fermentation by Phlebia sp. MG-60 is affected by water content and benefits from a small amount of aeration.http://ojs.cnr.ncsu.edu/index.php/BioRes/article/view/BioRes_09_3_5114_Kamei_Fungal_Production_Ethanol_FungusWhite-rot fungiBioethanolConsolidated bioprocessingWaste paper fermentation |
| spellingShingle | Ichiro Kamei Yoshiyuki Hirota Sadatoshi Meguro Direct Fungal Production of Ethanol from High-Solids Pulps by the Ethanol-fermenting White-rot Fungus Phlebia sp. MG-60 BioResources White-rot fungi Bioethanol Consolidated bioprocessing Waste paper fermentation |
| title | Direct Fungal Production of Ethanol from High-Solids Pulps by the Ethanol-fermenting White-rot Fungus Phlebia sp. MG-60 |
| title_full | Direct Fungal Production of Ethanol from High-Solids Pulps by the Ethanol-fermenting White-rot Fungus Phlebia sp. MG-60 |
| title_fullStr | Direct Fungal Production of Ethanol from High-Solids Pulps by the Ethanol-fermenting White-rot Fungus Phlebia sp. MG-60 |
| title_full_unstemmed | Direct Fungal Production of Ethanol from High-Solids Pulps by the Ethanol-fermenting White-rot Fungus Phlebia sp. MG-60 |
| title_short | Direct Fungal Production of Ethanol from High-Solids Pulps by the Ethanol-fermenting White-rot Fungus Phlebia sp. MG-60 |
| title_sort | direct fungal production of ethanol from high solids pulps by the ethanol fermenting white rot fungus phlebia sp mg 60 |
| topic | White-rot fungi Bioethanol Consolidated bioprocessing Waste paper fermentation |
| url | http://ojs.cnr.ncsu.edu/index.php/BioRes/article/view/BioRes_09_3_5114_Kamei_Fungal_Production_Ethanol_Fungus |
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