Nucleotide Imbalance, Provoked by Downregulation of Aspartate Transcarbamoylase Impairs Cold Acclimation in Arabidopsis
Aspartate transcarbamoylase (<i>ATC</i>) catalyzes the first committed step in pyrimidine de novo synthesis. As shown before, mutants with 80% reduced transcript and protein levels exhibit reduced levels of pyrimidine metabolites and thus nucleotide limitation and imbalance. Consequently...
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MDPI AG
2023-02-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/28/4/1585 |
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| author | Leo Bellin Diana Laura Garza Amaya Vanessa Scherer Tobias Pruß Annalisa John Andreas Richter Torsten Möhlmann |
| author_facet | Leo Bellin Diana Laura Garza Amaya Vanessa Scherer Tobias Pruß Annalisa John Andreas Richter Torsten Möhlmann |
| author_sort | Leo Bellin |
| collection | DOAJ |
| description | Aspartate transcarbamoylase (<i>ATC</i>) catalyzes the first committed step in pyrimidine de novo synthesis. As shown before, mutants with 80% reduced transcript and protein levels exhibit reduced levels of pyrimidine metabolites and thus nucleotide limitation and imbalance. Consequently, reduced photosynthetic capacity and growth, accompanied by massive transcriptional changes, were observed. Here, we show that nucleotide de novo synthesis was upregulated during cold acclimation of <i>Arabidopsis thaliana</i> (ecotype Columbia, Col-0) plants, but <i>ATC</i> knockdown mutants failed to acclimate to this condition as they did not accumulate neutral sugars and anthocyanins. A global transcriptome analysis revealed that most of the transcriptional changes observed in Col-0 plants upon cold exposure were also evident in <i>ATC</i> knockdown plants. However, several responses observed in cold-treated Col-0 plants could already be detected in knockdown plants when grown under standard conditions, suggesting that these mutants exhibited typical cold responses without prior cold stimulation. We believe that nucleotide signaling is involved in “cold-like priming” and “cold acclimation” in general. The observed transcript levels of genes involved in central carbon metabolism and respiration were an exception to these findings. These were upregulated in the cold but downregulated in warm-grown <i>ATC</i> mutants. |
| first_indexed | 2024-03-11T08:21:43Z |
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| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-11T08:21:43Z |
| publishDate | 2023-02-01 |
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| spelling | doaj.art-0c12a7f191674691a9a14a101bb0ac782023-11-16T22:20:30ZengMDPI AGMolecules1420-30492023-02-01284158510.3390/molecules28041585Nucleotide Imbalance, Provoked by Downregulation of Aspartate Transcarbamoylase Impairs Cold Acclimation in ArabidopsisLeo Bellin0Diana Laura Garza Amaya1Vanessa Scherer2Tobias Pruß3Annalisa John4Andreas Richter5Torsten Möhlmann6Faculty of Biology, Plant Physiology, University of Kaiserslautern, Erwin-Schrödinger-Straße, D-67663 Kaiserslautern, GermanyFaculty of Biology, Plant Physiology, University of Kaiserslautern, Erwin-Schrödinger-Straße, D-67663 Kaiserslautern, GermanyFaculty of Biology, Plant Physiology, University of Kaiserslautern, Erwin-Schrödinger-Straße, D-67663 Kaiserslautern, GermanyFaculty of Biology, Plant Physiology, University of Kaiserslautern, Erwin-Schrödinger-Straße, D-67663 Kaiserslautern, GermanyFaculty of Biology, Plant Physiology, University of Kaiserslautern, Erwin-Schrödinger-Straße, D-67663 Kaiserslautern, GermanyInstitute for Biosciences, Physiology of Plant Metabolism, University of Rostock, Albert-Einstein-Strasse 3, D-18059 Rostock, GermanyFaculty of Biology, Plant Physiology, University of Kaiserslautern, Erwin-Schrödinger-Straße, D-67663 Kaiserslautern, GermanyAspartate transcarbamoylase (<i>ATC</i>) catalyzes the first committed step in pyrimidine de novo synthesis. As shown before, mutants with 80% reduced transcript and protein levels exhibit reduced levels of pyrimidine metabolites and thus nucleotide limitation and imbalance. Consequently, reduced photosynthetic capacity and growth, accompanied by massive transcriptional changes, were observed. Here, we show that nucleotide de novo synthesis was upregulated during cold acclimation of <i>Arabidopsis thaliana</i> (ecotype Columbia, Col-0) plants, but <i>ATC</i> knockdown mutants failed to acclimate to this condition as they did not accumulate neutral sugars and anthocyanins. A global transcriptome analysis revealed that most of the transcriptional changes observed in Col-0 plants upon cold exposure were also evident in <i>ATC</i> knockdown plants. However, several responses observed in cold-treated Col-0 plants could already be detected in knockdown plants when grown under standard conditions, suggesting that these mutants exhibited typical cold responses without prior cold stimulation. We believe that nucleotide signaling is involved in “cold-like priming” and “cold acclimation” in general. The observed transcript levels of genes involved in central carbon metabolism and respiration were an exception to these findings. These were upregulated in the cold but downregulated in warm-grown <i>ATC</i> mutants.https://www.mdpi.com/1420-3049/28/4/1585pyrimidine de novo synthesiscold acclimationtranscriptomics |
| spellingShingle | Leo Bellin Diana Laura Garza Amaya Vanessa Scherer Tobias Pruß Annalisa John Andreas Richter Torsten Möhlmann Nucleotide Imbalance, Provoked by Downregulation of Aspartate Transcarbamoylase Impairs Cold Acclimation in Arabidopsis Molecules pyrimidine de novo synthesis cold acclimation transcriptomics |
| title | Nucleotide Imbalance, Provoked by Downregulation of Aspartate Transcarbamoylase Impairs Cold Acclimation in Arabidopsis |
| title_full | Nucleotide Imbalance, Provoked by Downregulation of Aspartate Transcarbamoylase Impairs Cold Acclimation in Arabidopsis |
| title_fullStr | Nucleotide Imbalance, Provoked by Downregulation of Aspartate Transcarbamoylase Impairs Cold Acclimation in Arabidopsis |
| title_full_unstemmed | Nucleotide Imbalance, Provoked by Downregulation of Aspartate Transcarbamoylase Impairs Cold Acclimation in Arabidopsis |
| title_short | Nucleotide Imbalance, Provoked by Downregulation of Aspartate Transcarbamoylase Impairs Cold Acclimation in Arabidopsis |
| title_sort | nucleotide imbalance provoked by downregulation of aspartate transcarbamoylase impairs cold acclimation in arabidopsis |
| topic | pyrimidine de novo synthesis cold acclimation transcriptomics |
| url | https://www.mdpi.com/1420-3049/28/4/1585 |
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