A Review of the Nucleic Acid-Based Lateral Flow Assay for Detection of Breast Cancer from Circulating Biomarkers at a Point-of-Care in Low Income Countries
The current levels of breast cancer in African women have contributed to the high mortality rates among them. In South Africa, the incidence of breast cancer is also on the rise due to changes in behavioural and biological risk factors. Such low survival rates can be attributed to the late diagnosis...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-08-01
|
| Series: | Diagnostics |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2075-4418/12/8/1973 |
| _version_ | 1827617973369569280 |
|---|---|
| author | Busiswa Dyan Palesa Pamela Seele Amanda Skepu Phumlane Selby Mdluli Salerwe Mosebi Nicole Remaliah Samantha Sibuyi |
| author_facet | Busiswa Dyan Palesa Pamela Seele Amanda Skepu Phumlane Selby Mdluli Salerwe Mosebi Nicole Remaliah Samantha Sibuyi |
| author_sort | Busiswa Dyan |
| collection | DOAJ |
| description | The current levels of breast cancer in African women have contributed to the high mortality rates among them. In South Africa, the incidence of breast cancer is also on the rise due to changes in behavioural and biological risk factors. Such low survival rates can be attributed to the late diagnosis of the disease due to a lack of access and the high costs of the current diagnostic tools. Breast cancer is asymptomatic at early stages, which is the best time to detect it and intervene to prevent high mortality rates. Proper risk assessment, campaigns, and access to adequate healthcare need to be prioritised among patients at an early stage. Early detection of breast cancer can significantly improve the survival rate of breast cancer patients, since therapeutic strategies are more effective at this stage. Early detection of breast cancer can be achieved by developing devices that are simple, sensitive, low-cost, and employed at point-of-care (POC), especially in low-income countries (LICs). Nucleic-acid-based lateral flow assays (NABLFAs) that combine molecular detection with the immunochemical visualisation principles, have recently emerged as tools for disease diagnosis, even for low biomarker concentrations. Detection of circulating genetic biomarkers in non-invasively collected biological fluids with NABLFAs presents an appealing and suitable method for POC testing in resource-limited regions and/or LICs. Diagnosis of breast cancer at an early stage will improve the survival rates of the patients. This review covers the analysis of the current state of NABLFA technologies used in developing countries to reduce the scourge of breast cancer. |
| first_indexed | 2024-03-09T09:58:00Z |
| format | Article |
| id | doaj.art-0c1408faf7a146b5b01087034433b983 |
| institution | Directory Open Access Journal |
| issn | 2075-4418 |
| language | English |
| last_indexed | 2024-03-09T09:58:00Z |
| publishDate | 2022-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Diagnostics |
| spelling | doaj.art-0c1408faf7a146b5b01087034433b9832023-12-01T23:36:42ZengMDPI AGDiagnostics2075-44182022-08-01128197310.3390/diagnostics12081973A Review of the Nucleic Acid-Based Lateral Flow Assay for Detection of Breast Cancer from Circulating Biomarkers at a Point-of-Care in Low Income CountriesBusiswa Dyan0Palesa Pamela Seele1Amanda Skepu2Phumlane Selby Mdluli3Salerwe Mosebi4Nicole Remaliah Samantha Sibuyi5Nanotechnology Innovation Centre, Health Platform, Mintek, 200 Malibongwe Drive, Randburg, Johannesburg 2194, South AfricaNanotechnology Innovation Centre, Health Platform, Mintek, 200 Malibongwe Drive, Randburg, Johannesburg 2194, South AfricaNanotechnology Innovation Centre, Health Platform, Mintek, 200 Malibongwe Drive, Randburg, Johannesburg 2194, South AfricaNanotechnology Innovation Centre, Health Platform, Mintek, 200 Malibongwe Drive, Randburg, Johannesburg 2194, South AfricaDepartment of Life and Consumer Sciences, College of Agriculture and Environmental Sciences, University of South Africa, Private Bag X6, Florida, Johannesburg 1710, South AfricaNanotechnology Innovation Centre, Health Platform, Mintek, 200 Malibongwe Drive, Randburg, Johannesburg 2194, South AfricaThe current levels of breast cancer in African women have contributed to the high mortality rates among them. In South Africa, the incidence of breast cancer is also on the rise due to changes in behavioural and biological risk factors. Such low survival rates can be attributed to the late diagnosis of the disease due to a lack of access and the high costs of the current diagnostic tools. Breast cancer is asymptomatic at early stages, which is the best time to detect it and intervene to prevent high mortality rates. Proper risk assessment, campaigns, and access to adequate healthcare need to be prioritised among patients at an early stage. Early detection of breast cancer can significantly improve the survival rate of breast cancer patients, since therapeutic strategies are more effective at this stage. Early detection of breast cancer can be achieved by developing devices that are simple, sensitive, low-cost, and employed at point-of-care (POC), especially in low-income countries (LICs). Nucleic-acid-based lateral flow assays (NABLFAs) that combine molecular detection with the immunochemical visualisation principles, have recently emerged as tools for disease diagnosis, even for low biomarker concentrations. Detection of circulating genetic biomarkers in non-invasively collected biological fluids with NABLFAs presents an appealing and suitable method for POC testing in resource-limited regions and/or LICs. Diagnosis of breast cancer at an early stage will improve the survival rates of the patients. This review covers the analysis of the current state of NABLFA technologies used in developing countries to reduce the scourge of breast cancer.https://www.mdpi.com/2075-4418/12/8/1973breast cancerNABLFAcirculating biomarkerspoint-of-carelow income countries |
| spellingShingle | Busiswa Dyan Palesa Pamela Seele Amanda Skepu Phumlane Selby Mdluli Salerwe Mosebi Nicole Remaliah Samantha Sibuyi A Review of the Nucleic Acid-Based Lateral Flow Assay for Detection of Breast Cancer from Circulating Biomarkers at a Point-of-Care in Low Income Countries Diagnostics breast cancer NABLFA circulating biomarkers point-of-care low income countries |
| title | A Review of the Nucleic Acid-Based Lateral Flow Assay for Detection of Breast Cancer from Circulating Biomarkers at a Point-of-Care in Low Income Countries |
| title_full | A Review of the Nucleic Acid-Based Lateral Flow Assay for Detection of Breast Cancer from Circulating Biomarkers at a Point-of-Care in Low Income Countries |
| title_fullStr | A Review of the Nucleic Acid-Based Lateral Flow Assay for Detection of Breast Cancer from Circulating Biomarkers at a Point-of-Care in Low Income Countries |
| title_full_unstemmed | A Review of the Nucleic Acid-Based Lateral Flow Assay for Detection of Breast Cancer from Circulating Biomarkers at a Point-of-Care in Low Income Countries |
| title_short | A Review of the Nucleic Acid-Based Lateral Flow Assay for Detection of Breast Cancer from Circulating Biomarkers at a Point-of-Care in Low Income Countries |
| title_sort | review of the nucleic acid based lateral flow assay for detection of breast cancer from circulating biomarkers at a point of care in low income countries |
| topic | breast cancer NABLFA circulating biomarkers point-of-care low income countries |
| url | https://www.mdpi.com/2075-4418/12/8/1973 |
| work_keys_str_mv | AT busiswadyan areviewofthenucleicacidbasedlateralflowassayfordetectionofbreastcancerfromcirculatingbiomarkersatapointofcareinlowincomecountries AT palesapamelaseele areviewofthenucleicacidbasedlateralflowassayfordetectionofbreastcancerfromcirculatingbiomarkersatapointofcareinlowincomecountries AT amandaskepu areviewofthenucleicacidbasedlateralflowassayfordetectionofbreastcancerfromcirculatingbiomarkersatapointofcareinlowincomecountries AT phumlaneselbymdluli areviewofthenucleicacidbasedlateralflowassayfordetectionofbreastcancerfromcirculatingbiomarkersatapointofcareinlowincomecountries AT salerwemosebi areviewofthenucleicacidbasedlateralflowassayfordetectionofbreastcancerfromcirculatingbiomarkersatapointofcareinlowincomecountries AT nicoleremaliahsamanthasibuyi areviewofthenucleicacidbasedlateralflowassayfordetectionofbreastcancerfromcirculatingbiomarkersatapointofcareinlowincomecountries AT busiswadyan reviewofthenucleicacidbasedlateralflowassayfordetectionofbreastcancerfromcirculatingbiomarkersatapointofcareinlowincomecountries AT palesapamelaseele reviewofthenucleicacidbasedlateralflowassayfordetectionofbreastcancerfromcirculatingbiomarkersatapointofcareinlowincomecountries AT amandaskepu reviewofthenucleicacidbasedlateralflowassayfordetectionofbreastcancerfromcirculatingbiomarkersatapointofcareinlowincomecountries AT phumlaneselbymdluli reviewofthenucleicacidbasedlateralflowassayfordetectionofbreastcancerfromcirculatingbiomarkersatapointofcareinlowincomecountries AT salerwemosebi reviewofthenucleicacidbasedlateralflowassayfordetectionofbreastcancerfromcirculatingbiomarkersatapointofcareinlowincomecountries AT nicoleremaliahsamanthasibuyi reviewofthenucleicacidbasedlateralflowassayfordetectionofbreastcancerfromcirculatingbiomarkersatapointofcareinlowincomecountries |