Metabarcoding data of mitochondrial cytochrome c oxidase subunit 1 gene from bulk community of aquatic organisms collected from Nara Prefecture, Japan
Riverine metabarcoding data were obtained from the Takamigawa River, a tributary of the Kinokawa River, in Nara Prefecture (Central Honshu, Japan). We extracted DNA from bulk community samples of aquatic organisms, most of which could not be morphologically identified at species level due to their s...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2022-12-01
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| Series: | Data in Brief |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S235234092200806X |
| _version_ | 1811190095454142464 |
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| author | Kei Wakimura Koji Inai Kazumi Tanida Kozo Watanabe Mikio Kato |
| author_facet | Kei Wakimura Koji Inai Kazumi Tanida Kozo Watanabe Mikio Kato |
| author_sort | Kei Wakimura |
| collection | DOAJ |
| description | Riverine metabarcoding data were obtained from the Takamigawa River, a tributary of the Kinokawa River, in Nara Prefecture (Central Honshu, Japan). We extracted DNA from bulk community samples of aquatic organisms, most of which could not be morphologically identified at species level due to their small body size (0.12 - 2 mm length). A partial coding region of the mitochondrial cytochrome c oxidase subunit 1 gene (cox1) was amplified using PCR, and the amplicon was subjected to high-throughput parallel sequencing (Illumina MiSeq). The 313 bp paired-end sequence reads were classified into operational taxonomic units (OTUs), their species boundaries were delineated using the Generalised Mixed Yule Coalescent (GMYC) method, and taxonomic names of the GMYC species were assigned using basic local alignment search tool (BLAST) against International DNA Databases (INSD: GenBank, ENA, and DDBJ). |
| first_indexed | 2024-04-11T14:45:40Z |
| format | Article |
| id | doaj.art-0c14dcb3855a43c783bf0bcc4741b770 |
| institution | Directory Open Access Journal |
| issn | 2352-3409 |
| language | English |
| last_indexed | 2024-04-11T14:45:40Z |
| publishDate | 2022-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Data in Brief |
| spelling | doaj.art-0c14dcb3855a43c783bf0bcc4741b7702022-12-22T04:17:40ZengElsevierData in Brief2352-34092022-12-0145108599Metabarcoding data of mitochondrial cytochrome c oxidase subunit 1 gene from bulk community of aquatic organisms collected from Nara Prefecture, JapanKei Wakimura0Koji Inai1Kazumi Tanida2Kozo Watanabe3Mikio Kato4Faculty of Liberal Arts and Sciences, Osaka Metropolitan University - Nakamozu Campus, 1-1 Gakuencho, Sakai 599-8531, JapanFaculty of Liberal Arts and Sciences, Osaka Metropolitan University - Nakamozu Campus, 1-1 Gakuencho, Sakai 599-8531, JapanOsaka Museum of Natural History, Nagai Park, Higashi-sumiyoshi, Osaka, 546-0034, JapanCenter for Marine Environment Studies, Ehime University, Bunkyo-cho 3, Matsuyama 790-8577, JapanFaculty of Liberal Arts and Sciences, Osaka Metropolitan University - Nakamozu Campus, 1-1 Gakuencho, Sakai 599-8531, Japan; Department of Biology, Graduate School of Science, Osaka Metropolitan University - Nakamozu Campus, 1-1 Gakuencho, Sakai 599-8531, Japan; Corresponding author.Riverine metabarcoding data were obtained from the Takamigawa River, a tributary of the Kinokawa River, in Nara Prefecture (Central Honshu, Japan). We extracted DNA from bulk community samples of aquatic organisms, most of which could not be morphologically identified at species level due to their small body size (0.12 - 2 mm length). A partial coding region of the mitochondrial cytochrome c oxidase subunit 1 gene (cox1) was amplified using PCR, and the amplicon was subjected to high-throughput parallel sequencing (Illumina MiSeq). The 313 bp paired-end sequence reads were classified into operational taxonomic units (OTUs), their species boundaries were delineated using the Generalised Mixed Yule Coalescent (GMYC) method, and taxonomic names of the GMYC species were assigned using basic local alignment search tool (BLAST) against International DNA Databases (INSD: GenBank, ENA, and DDBJ).http://www.sciencedirect.com/science/article/pii/S235234092200806XBenthic faunaCytochrome c oxidase subunit 1 gene (cox1)MetabarcodingRiverine metagenomeSpecies identification |
| spellingShingle | Kei Wakimura Koji Inai Kazumi Tanida Kozo Watanabe Mikio Kato Metabarcoding data of mitochondrial cytochrome c oxidase subunit 1 gene from bulk community of aquatic organisms collected from Nara Prefecture, Japan Data in Brief Benthic fauna Cytochrome c oxidase subunit 1 gene (cox1) Metabarcoding Riverine metagenome Species identification |
| title | Metabarcoding data of mitochondrial cytochrome c oxidase subunit 1 gene from bulk community of aquatic organisms collected from Nara Prefecture, Japan |
| title_full | Metabarcoding data of mitochondrial cytochrome c oxidase subunit 1 gene from bulk community of aquatic organisms collected from Nara Prefecture, Japan |
| title_fullStr | Metabarcoding data of mitochondrial cytochrome c oxidase subunit 1 gene from bulk community of aquatic organisms collected from Nara Prefecture, Japan |
| title_full_unstemmed | Metabarcoding data of mitochondrial cytochrome c oxidase subunit 1 gene from bulk community of aquatic organisms collected from Nara Prefecture, Japan |
| title_short | Metabarcoding data of mitochondrial cytochrome c oxidase subunit 1 gene from bulk community of aquatic organisms collected from Nara Prefecture, Japan |
| title_sort | metabarcoding data of mitochondrial cytochrome c oxidase subunit 1 gene from bulk community of aquatic organisms collected from nara prefecture japan |
| topic | Benthic fauna Cytochrome c oxidase subunit 1 gene (cox1) Metabarcoding Riverine metagenome Species identification |
| url | http://www.sciencedirect.com/science/article/pii/S235234092200806X |
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