Biocompatibility Profile and In Vitro Cellular Uptake of Self-assembled Alginate Nanoparticles
Polymeric nanoparticles could offer promising controlled drug delivery. The biocompatibility is of extreme importance for future applications in humans. Self-assembled polymeric nanoparticles based on phenylalanine ethyl ester (PAE)-modified alginate (Alg) had been successfully prepared and characte...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2019-02-01
|
| Series: | Molecules |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1420-3049/24/3/555 |
| _version_ | 1818302305714307072 |
|---|---|
| author | Pei Zhang Shirui Zhao Yaoyao Yu Huan Wang Yan Yang Chenguang Liu |
| author_facet | Pei Zhang Shirui Zhao Yaoyao Yu Huan Wang Yan Yang Chenguang Liu |
| author_sort | Pei Zhang |
| collection | DOAJ |
| description | Polymeric nanoparticles could offer promising controlled drug delivery. The biocompatibility is of extreme importance for future applications in humans. Self-assembled polymeric nanoparticles based on phenylalanine ethyl ester (PAE)-modified alginate (Alg) had been successfully prepared and characterized in our lab. However, little is known about their interaction with cells and other biological systems. In this study, nanoparticles (NPs) based on PAE-Alg conjugates (PEA-NPs) with different degree of substitution (DS) were prepared and investigated. Our results showed that PEA-NPs had no effects on the proliferation of the human intestinal epithelial Caco-2 cells at concentrations up to 1000 μg/mL. Furthermore, the in vitro cellular uptake profile of PEA-NPs, concerning several parameters involved in the application of therapeutic or diagnostic NPs, such as NPs concentration, time and temperature, was described. Different NPs have been adopted for cellular uptake studies and the NPs internalized into Caco-2 cells were quantified. Cellular uptake efficiency could reach 60% within 4 h. PEA-NPs also showed greater cell permeability than oleoyl alginate ester nanoparticles (OAE-NPs) previously prepared in our lab. Our studies reveal that NPs based on PEA conjugate are promising nanosystems for cellular delivery. |
| first_indexed | 2024-12-13T05:36:48Z |
| format | Article |
| id | doaj.art-0c165eabad044b18bf627ec1481f7a93 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-12-13T05:36:48Z |
| publishDate | 2019-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-0c165eabad044b18bf627ec1481f7a932022-12-21T23:57:54ZengMDPI AGMolecules1420-30492019-02-0124355510.3390/molecules24030555molecules24030555Biocompatibility Profile and In Vitro Cellular Uptake of Self-assembled Alginate NanoparticlesPei Zhang0Shirui Zhao1Yaoyao Yu2Huan Wang3Yan Yang4Chenguang Liu5College of Marine Life Sciences, Ocean University of China, Qingdao 266003, ChinaCollege of Marine Life Sciences, Ocean University of China, Qingdao 266003, ChinaDepartment of Life Science, Luoyang Normal University, Luoyang 471934, ChinaDepartment of Life Science, Luoyang Normal University, Luoyang 471934, ChinaCollege of Marine Life Sciences, Ocean University of China, Qingdao 266003, ChinaCollege of Marine Life Sciences, Ocean University of China, Qingdao 266003, ChinaPolymeric nanoparticles could offer promising controlled drug delivery. The biocompatibility is of extreme importance for future applications in humans. Self-assembled polymeric nanoparticles based on phenylalanine ethyl ester (PAE)-modified alginate (Alg) had been successfully prepared and characterized in our lab. However, little is known about their interaction with cells and other biological systems. In this study, nanoparticles (NPs) based on PAE-Alg conjugates (PEA-NPs) with different degree of substitution (DS) were prepared and investigated. Our results showed that PEA-NPs had no effects on the proliferation of the human intestinal epithelial Caco-2 cells at concentrations up to 1000 μg/mL. Furthermore, the in vitro cellular uptake profile of PEA-NPs, concerning several parameters involved in the application of therapeutic or diagnostic NPs, such as NPs concentration, time and temperature, was described. Different NPs have been adopted for cellular uptake studies and the NPs internalized into Caco-2 cells were quantified. Cellular uptake efficiency could reach 60% within 4 h. PEA-NPs also showed greater cell permeability than oleoyl alginate ester nanoparticles (OAE-NPs) previously prepared in our lab. Our studies reveal that NPs based on PEA conjugate are promising nanosystems for cellular delivery.https://www.mdpi.com/1420-3049/24/3/555alginatedegree of substitutionnanoparticlescytotoxicitycellular uptake |
| spellingShingle | Pei Zhang Shirui Zhao Yaoyao Yu Huan Wang Yan Yang Chenguang Liu Biocompatibility Profile and In Vitro Cellular Uptake of Self-assembled Alginate Nanoparticles Molecules alginate degree of substitution nanoparticles cytotoxicity cellular uptake |
| title | Biocompatibility Profile and In Vitro Cellular Uptake of Self-assembled Alginate Nanoparticles |
| title_full | Biocompatibility Profile and In Vitro Cellular Uptake of Self-assembled Alginate Nanoparticles |
| title_fullStr | Biocompatibility Profile and In Vitro Cellular Uptake of Self-assembled Alginate Nanoparticles |
| title_full_unstemmed | Biocompatibility Profile and In Vitro Cellular Uptake of Self-assembled Alginate Nanoparticles |
| title_short | Biocompatibility Profile and In Vitro Cellular Uptake of Self-assembled Alginate Nanoparticles |
| title_sort | biocompatibility profile and in vitro cellular uptake of self assembled alginate nanoparticles |
| topic | alginate degree of substitution nanoparticles cytotoxicity cellular uptake |
| url | https://www.mdpi.com/1420-3049/24/3/555 |
| work_keys_str_mv | AT peizhang biocompatibilityprofileandinvitrocellularuptakeofselfassembledalginatenanoparticles AT shiruizhao biocompatibilityprofileandinvitrocellularuptakeofselfassembledalginatenanoparticles AT yaoyaoyu biocompatibilityprofileandinvitrocellularuptakeofselfassembledalginatenanoparticles AT huanwang biocompatibilityprofileandinvitrocellularuptakeofselfassembledalginatenanoparticles AT yanyang biocompatibilityprofileandinvitrocellularuptakeofselfassembledalginatenanoparticles AT chenguangliu biocompatibilityprofileandinvitrocellularuptakeofselfassembledalginatenanoparticles |