The effects of resveratrol on the expression of VEGF, TGF-β, and MMP-9 in endometrial stromal cells of women with endometriosis
Abstract Resveratrol is a phytochemical with anti-angiogenic, anti-inflammatory, and antioxidant properties. The present study has evaluated the effect of resveratrol on the expression of vascular endothelial growth factor (VEGF), transforming growth factor-β (TGF-β) and matrix metalloproteinase-9 (...
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Nature Portfolio
2021-03-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-021-85512-y |
| _version_ | 1818425627193114624 |
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| author | Tahereh Arablou Naheed Aryaeian Sepideh Khodaverdi Roya Kolahdouz-Mohammadi Zahra Moradi Nesa Rashidi Ali-Akbar Delbandi |
| author_facet | Tahereh Arablou Naheed Aryaeian Sepideh Khodaverdi Roya Kolahdouz-Mohammadi Zahra Moradi Nesa Rashidi Ali-Akbar Delbandi |
| author_sort | Tahereh Arablou |
| collection | DOAJ |
| description | Abstract Resveratrol is a phytochemical with anti-angiogenic, anti-inflammatory, and antioxidant properties. The present study has evaluated the effect of resveratrol on the expression of vascular endothelial growth factor (VEGF), transforming growth factor-β (TGF-β) and matrix metalloproteinase-9 (MMP-9) as factors related to endometriosis progression. Thirteen eutopic (EuESCs) and 8 ectopic (EESCs) endometrial stromal cells from women with endometriosis and 11 control endometrial stromal cells (CESCs) were treated with resveratrol (100 µM) for 6, 24 and 48 h. The gene and protein expression levels of VEGF, TGF-β, and MMP-9 were measured using real-time PCR and ELISA methods, respectively. Results showed that the basal gene and protein expression of VEGF and MMP-9 were higher in EESCs compared to EuESCs and CESCs (P < 0.01 to < 0.001 and P < 0.05 to < 0.01 respectively). Also, resveratrol treatment decreased the gene and protein expression of VEGF and MMP-9 in EuESCs, EESCs and CESCs (P < 0.05 to < 0.01 and P < 0.05 to < 0.01 respectively) and gene and protein expression of TGF-β in EESCs and EuESCs (P < 0.05 to < 0.01). The effect of resveratrol in reduction of VEGF gene expression was statistically more noticeable in EESCs compared to EuESCs and CESCs (P < 0.05). According to the findings, resveratrol may ameliorate endometriosis progression through reducing the expression of VEGF, TGF-β, and MMP-9 in endometrial stromal cells (ESCs). |
| first_indexed | 2024-12-14T14:16:56Z |
| format | Article |
| id | doaj.art-0c1a9da73bb24f67b1751969d4f3aa20 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-14T14:16:56Z |
| publishDate | 2021-03-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-0c1a9da73bb24f67b1751969d4f3aa202022-12-21T22:58:11ZengNature PortfolioScientific Reports2045-23222021-03-0111111210.1038/s41598-021-85512-yThe effects of resveratrol on the expression of VEGF, TGF-β, and MMP-9 in endometrial stromal cells of women with endometriosisTahereh Arablou0Naheed Aryaeian1Sepideh Khodaverdi2Roya Kolahdouz-Mohammadi3Zahra Moradi4Nesa Rashidi5Ali-Akbar Delbandi6Department of Nutrition, School of Public Health, Iran University of Medical SciencesDepartment of Nutrition, School of Public Health, Iran University of Medical SciencesEndometriosis Research Center, Iran University of Medical ScienceDepartment of Nutrition, School of Public Health, Iran University of Medical SciencesDepartment of Immunology, School of Medicine, Iran University of Medical SciencesDepartment of Immunology, School of Medicine, Iran University of Medical SciencesDepartment of Immunology, School of Medicine, Iran University of Medical SciencesAbstract Resveratrol is a phytochemical with anti-angiogenic, anti-inflammatory, and antioxidant properties. The present study has evaluated the effect of resveratrol on the expression of vascular endothelial growth factor (VEGF), transforming growth factor-β (TGF-β) and matrix metalloproteinase-9 (MMP-9) as factors related to endometriosis progression. Thirteen eutopic (EuESCs) and 8 ectopic (EESCs) endometrial stromal cells from women with endometriosis and 11 control endometrial stromal cells (CESCs) were treated with resveratrol (100 µM) for 6, 24 and 48 h. The gene and protein expression levels of VEGF, TGF-β, and MMP-9 were measured using real-time PCR and ELISA methods, respectively. Results showed that the basal gene and protein expression of VEGF and MMP-9 were higher in EESCs compared to EuESCs and CESCs (P < 0.01 to < 0.001 and P < 0.05 to < 0.01 respectively). Also, resveratrol treatment decreased the gene and protein expression of VEGF and MMP-9 in EuESCs, EESCs and CESCs (P < 0.05 to < 0.01 and P < 0.05 to < 0.01 respectively) and gene and protein expression of TGF-β in EESCs and EuESCs (P < 0.05 to < 0.01). The effect of resveratrol in reduction of VEGF gene expression was statistically more noticeable in EESCs compared to EuESCs and CESCs (P < 0.05). According to the findings, resveratrol may ameliorate endometriosis progression through reducing the expression of VEGF, TGF-β, and MMP-9 in endometrial stromal cells (ESCs).https://doi.org/10.1038/s41598-021-85512-y |
| spellingShingle | Tahereh Arablou Naheed Aryaeian Sepideh Khodaverdi Roya Kolahdouz-Mohammadi Zahra Moradi Nesa Rashidi Ali-Akbar Delbandi The effects of resveratrol on the expression of VEGF, TGF-β, and MMP-9 in endometrial stromal cells of women with endometriosis Scientific Reports |
| title | The effects of resveratrol on the expression of VEGF, TGF-β, and MMP-9 in endometrial stromal cells of women with endometriosis |
| title_full | The effects of resveratrol on the expression of VEGF, TGF-β, and MMP-9 in endometrial stromal cells of women with endometriosis |
| title_fullStr | The effects of resveratrol on the expression of VEGF, TGF-β, and MMP-9 in endometrial stromal cells of women with endometriosis |
| title_full_unstemmed | The effects of resveratrol on the expression of VEGF, TGF-β, and MMP-9 in endometrial stromal cells of women with endometriosis |
| title_short | The effects of resveratrol on the expression of VEGF, TGF-β, and MMP-9 in endometrial stromal cells of women with endometriosis |
| title_sort | effects of resveratrol on the expression of vegf tgf β and mmp 9 in endometrial stromal cells of women with endometriosis |
| url | https://doi.org/10.1038/s41598-021-85512-y |
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