An Increase in Mucin2 Expression Is Required for Colon Cancer Progression Mediated by L1
An induction in the expression of the cell adhesion receptor L1, a Wnt target gene, is a characteristic feature of Wnt/β-catenin activation in colon cancer cells at later stages of the disease. We investigated the proteins secreted following L1 expression in colon cancer cells and identified Mucin2...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-08-01
|
| Series: | International Journal of Molecular Sciences |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1422-0067/24/17/13418 |
| _version_ | 1797582364286648320 |
|---|---|
| author | Arka Saha Nancy Gavert Thomas Brabletz Avri Ben-Ze’ev |
| author_facet | Arka Saha Nancy Gavert Thomas Brabletz Avri Ben-Ze’ev |
| author_sort | Arka Saha |
| collection | DOAJ |
| description | An induction in the expression of the cell adhesion receptor L1, a Wnt target gene, is a characteristic feature of Wnt/β-catenin activation in colon cancer cells at later stages of the disease. We investigated the proteins secreted following L1 expression in colon cancer cells and identified Mucin2 among the most abundant secreted proteins. We found that suppressing Mucin2 expression in L1-expressing colon cancer cells inhibits cell proliferation, motility, tumorigenesis, and liver metastasis. We detected several signaling pathways involved in Mucin2 induction in L1-expressing cells. In human colon cancer tissue, Mucin2 expression was significantly reduced or lost in the adenocarcinoma tissue, while in the mucinous subtype of colon cancer tissue, Mucin2 expression was increased. An increased signature of <i>L1</i>/<i>Mucin2</i> expression reduced the survival rate of human colon cancer patients. Thus, induction of Mucin2 expression by L1 is required during mucinous colon cancer progression and can serve as a marker for diagnosis and a target for therapy<b>.</b> |
| first_indexed | 2024-03-10T23:21:06Z |
| format | Article |
| id | doaj.art-0c1d3c28290b4dcaa771884f61c65882 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T23:21:06Z |
| publishDate | 2023-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-0c1d3c28290b4dcaa771884f61c658822023-11-19T08:17:01ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-08-0124171341810.3390/ijms241713418An Increase in Mucin2 Expression Is Required for Colon Cancer Progression Mediated by L1Arka Saha0Nancy Gavert1Thomas Brabletz2Avri Ben-Ze’ev3Department of Molecular Cell Biology, Weizmann Institute of Science, Rehovot 7610001, IsraelDepartment of Molecular Cell Biology, Weizmann Institute of Science, Rehovot 7610001, IsraelDepartment of Experimental Medicine I, Nikolaus-Feibiger-Center for Molecular Medicine, University of Erlangen-Nuernberg, 91054 Erlangen, GermanyDepartment of Molecular Cell Biology, Weizmann Institute of Science, Rehovot 7610001, IsraelAn induction in the expression of the cell adhesion receptor L1, a Wnt target gene, is a characteristic feature of Wnt/β-catenin activation in colon cancer cells at later stages of the disease. We investigated the proteins secreted following L1 expression in colon cancer cells and identified Mucin2 among the most abundant secreted proteins. We found that suppressing Mucin2 expression in L1-expressing colon cancer cells inhibits cell proliferation, motility, tumorigenesis, and liver metastasis. We detected several signaling pathways involved in Mucin2 induction in L1-expressing cells. In human colon cancer tissue, Mucin2 expression was significantly reduced or lost in the adenocarcinoma tissue, while in the mucinous subtype of colon cancer tissue, Mucin2 expression was increased. An increased signature of <i>L1</i>/<i>Mucin2</i> expression reduced the survival rate of human colon cancer patients. Thus, induction of Mucin2 expression by L1 is required during mucinous colon cancer progression and can serve as a marker for diagnosis and a target for therapy<b>.</b>https://www.mdpi.com/1422-0067/24/17/13418Mucin2L1colon cancertumorigenesismetastasis |
| spellingShingle | Arka Saha Nancy Gavert Thomas Brabletz Avri Ben-Ze’ev An Increase in Mucin2 Expression Is Required for Colon Cancer Progression Mediated by L1 International Journal of Molecular Sciences Mucin2 L1 colon cancer tumorigenesis metastasis |
| title | An Increase in Mucin2 Expression Is Required for Colon Cancer Progression Mediated by L1 |
| title_full | An Increase in Mucin2 Expression Is Required for Colon Cancer Progression Mediated by L1 |
| title_fullStr | An Increase in Mucin2 Expression Is Required for Colon Cancer Progression Mediated by L1 |
| title_full_unstemmed | An Increase in Mucin2 Expression Is Required for Colon Cancer Progression Mediated by L1 |
| title_short | An Increase in Mucin2 Expression Is Required for Colon Cancer Progression Mediated by L1 |
| title_sort | increase in mucin2 expression is required for colon cancer progression mediated by l1 |
| topic | Mucin2 L1 colon cancer tumorigenesis metastasis |
| url | https://www.mdpi.com/1422-0067/24/17/13418 |
| work_keys_str_mv | AT arkasaha anincreaseinmucin2expressionisrequiredforcoloncancerprogressionmediatedbyl1 AT nancygavert anincreaseinmucin2expressionisrequiredforcoloncancerprogressionmediatedbyl1 AT thomasbrabletz anincreaseinmucin2expressionisrequiredforcoloncancerprogressionmediatedbyl1 AT avribenzeev anincreaseinmucin2expressionisrequiredforcoloncancerprogressionmediatedbyl1 AT arkasaha increaseinmucin2expressionisrequiredforcoloncancerprogressionmediatedbyl1 AT nancygavert increaseinmucin2expressionisrequiredforcoloncancerprogressionmediatedbyl1 AT thomasbrabletz increaseinmucin2expressionisrequiredforcoloncancerprogressionmediatedbyl1 AT avribenzeev increaseinmucin2expressionisrequiredforcoloncancerprogressionmediatedbyl1 |