Comparison of VSV Pseudovirus and Focus Reduction Neutralization Assays for Measurement of Anti-Andes orthohantavirus Neutralizing Antibodies in Patient Samples
Andes orthohantavirus (ANDV) is the etiologic agent of hantavirus cardiopulmonary syndrome (HCPS), which has a case fatality rate around 35%, with no effective treatment or vaccine available. ANDV neutralizing antibody (NAb) measurements are important for the evaluation of the immune response follow...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2020-09-01
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| Series: | Frontiers in Cellular and Infection Microbiology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/article/10.3389/fcimb.2020.00444/full |
| _version_ | 1818980474801881088 |
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| author | Cecilia Vial Annalis Whitaker Annalis Whitaker Jan Wilhelm Jan Wilhelm Jimena Ovalle Ruth Perez Francisca Valdivieso Marcela Ferres Constanza Martinez-Valdebenito Philip Eisenhauer Gregory J. Mertz Jay W. Hooper Jason W. Botten Jason W. Botten Pablo A. Vial Pablo A. Vial |
| author_facet | Cecilia Vial Annalis Whitaker Annalis Whitaker Jan Wilhelm Jan Wilhelm Jimena Ovalle Ruth Perez Francisca Valdivieso Marcela Ferres Constanza Martinez-Valdebenito Philip Eisenhauer Gregory J. Mertz Jay W. Hooper Jason W. Botten Jason W. Botten Pablo A. Vial Pablo A. Vial |
| author_sort | Cecilia Vial |
| collection | DOAJ |
| description | Andes orthohantavirus (ANDV) is the etiologic agent of hantavirus cardiopulmonary syndrome (HCPS), which has a case fatality rate around 35%, with no effective treatment or vaccine available. ANDV neutralizing antibody (NAb) measurements are important for the evaluation of the immune response following infection, vaccination, or passive administration of investigational monoclonal or polyclonal antibodies. The standard assay for NAb measurement is a focus reduction neutralization test (FRNT) featuring live ANDV and must be completed under biosafety level (BSL)-3 conditions. In this study, we compared neutralization assays featuring infectious ANDV or vesicular stomatitis virus (VSV) pseudovirions decorated with ANDV glycoproteins for their ability to measure anti-ANDV NAbs from patient samples. Our studies demonstrate that VSV pseudovirions effectively measure NAb from clinical samples and have greater sensitivity compared to FRNT with live ANDV. Importantly, the pseudovirus assay requires less labor and sample materials and can be conducted at BSL-2. |
| first_indexed | 2024-12-20T17:16:00Z |
| format | Article |
| id | doaj.art-0c1ea6365436490dab9b319daf13683f |
| institution | Directory Open Access Journal |
| issn | 2235-2988 |
| language | English |
| last_indexed | 2024-12-20T17:16:00Z |
| publishDate | 2020-09-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cellular and Infection Microbiology |
| spelling | doaj.art-0c1ea6365436490dab9b319daf13683f2022-12-21T19:32:00ZengFrontiers Media S.A.Frontiers in Cellular and Infection Microbiology2235-29882020-09-011010.3389/fcimb.2020.00444557261Comparison of VSV Pseudovirus and Focus Reduction Neutralization Assays for Measurement of Anti-Andes orthohantavirus Neutralizing Antibodies in Patient SamplesCecilia Vial0Annalis Whitaker1Annalis Whitaker2Jan Wilhelm3Jan Wilhelm4Jimena Ovalle5Ruth Perez6Francisca Valdivieso7Marcela Ferres8Constanza Martinez-Valdebenito9Philip Eisenhauer10Gregory J. Mertz11Jay W. Hooper12Jason W. Botten13Jason W. Botten14Pablo A. Vial15Pablo A. Vial16Facultad de Medicina Clínica Alemana Universidad del Desarrollo, Programa Hantavirus, Instituto de Ciencias e Innovación en Medicina, Santiago, ChileDivision of Immunobiology, Department of Medicine, University of Vermont, Burlington, VT, United StatesCellular, Molecular and Biomedical Sciences Graduate Program, University of Vermont, Burlington, VT, United StatesFacultad de Medicina Clínica Alemana Universidad del Desarrollo, Programa Hantavirus, Instituto de Ciencias e Innovación en Medicina, Santiago, ChileClínica Alemana de Santiago, Santiago, ChileFacultad de Medicina Clínica Alemana Universidad del Desarrollo, Programa Hantavirus, Instituto de Ciencias e Innovación en Medicina, Santiago, ChileFacultad de Medicina Clínica Alemana Universidad del Desarrollo, Programa Hantavirus, Instituto de Ciencias e Innovación en Medicina, Santiago, ChileClínica Alemana de Santiago, Santiago, ChileLaboratorio de Infectología y Virología Molecular, Departamento de Enfermedades Infecciosas e Inmunología Pediátrica, Facultad de Medicina Pontificia Universidad Católica de Chile, Santiago, ChileLaboratorio de Infectología y Virología Molecular, Departamento de Enfermedades Infecciosas e Inmunología Pediátrica, Facultad de Medicina Pontificia Universidad Católica de Chile, Santiago, ChileDivision of Immunobiology, Department of Medicine, University of Vermont, Burlington, VT, United StatesDivision of Infectious Diseases, Department of Internal Medicine University of New Mexico, Albuquerque, NM, United StatesMolecular Virology Branch, United States Army Medical Research Institute of Infectious Diseases, Frederick, MD, United StatesDivision of Immunobiology, Department of Medicine, University of Vermont, Burlington, VT, United StatesDepartment of Microbiology and Molecular Genetics, University of Vermont, Burlington, VT, United StatesFacultad de Medicina Clínica Alemana Universidad del Desarrollo, Programa Hantavirus, Instituto de Ciencias e Innovación en Medicina, Santiago, ChileClínica Alemana de Santiago, Santiago, ChileAndes orthohantavirus (ANDV) is the etiologic agent of hantavirus cardiopulmonary syndrome (HCPS), which has a case fatality rate around 35%, with no effective treatment or vaccine available. ANDV neutralizing antibody (NAb) measurements are important for the evaluation of the immune response following infection, vaccination, or passive administration of investigational monoclonal or polyclonal antibodies. The standard assay for NAb measurement is a focus reduction neutralization test (FRNT) featuring live ANDV and must be completed under biosafety level (BSL)-3 conditions. In this study, we compared neutralization assays featuring infectious ANDV or vesicular stomatitis virus (VSV) pseudovirions decorated with ANDV glycoproteins for their ability to measure anti-ANDV NAbs from patient samples. Our studies demonstrate that VSV pseudovirions effectively measure NAb from clinical samples and have greater sensitivity compared to FRNT with live ANDV. Importantly, the pseudovirus assay requires less labor and sample materials and can be conducted at BSL-2.https://www.frontiersin.org/article/10.3389/fcimb.2020.00444/fullAndes orthohantavirus (ANDV)hantavirus cardiopulmonary syndrome (HCPS)orthohantavirusvesicular stomatitis virus (VSV) pseudovirusneutralizing antibodies |
| spellingShingle | Cecilia Vial Annalis Whitaker Annalis Whitaker Jan Wilhelm Jan Wilhelm Jimena Ovalle Ruth Perez Francisca Valdivieso Marcela Ferres Constanza Martinez-Valdebenito Philip Eisenhauer Gregory J. Mertz Jay W. Hooper Jason W. Botten Jason W. Botten Pablo A. Vial Pablo A. Vial Comparison of VSV Pseudovirus and Focus Reduction Neutralization Assays for Measurement of Anti-Andes orthohantavirus Neutralizing Antibodies in Patient Samples Frontiers in Cellular and Infection Microbiology Andes orthohantavirus (ANDV) hantavirus cardiopulmonary syndrome (HCPS) orthohantavirus vesicular stomatitis virus (VSV) pseudovirus neutralizing antibodies |
| title | Comparison of VSV Pseudovirus and Focus Reduction Neutralization Assays for Measurement of Anti-Andes orthohantavirus Neutralizing Antibodies in Patient Samples |
| title_full | Comparison of VSV Pseudovirus and Focus Reduction Neutralization Assays for Measurement of Anti-Andes orthohantavirus Neutralizing Antibodies in Patient Samples |
| title_fullStr | Comparison of VSV Pseudovirus and Focus Reduction Neutralization Assays for Measurement of Anti-Andes orthohantavirus Neutralizing Antibodies in Patient Samples |
| title_full_unstemmed | Comparison of VSV Pseudovirus and Focus Reduction Neutralization Assays for Measurement of Anti-Andes orthohantavirus Neutralizing Antibodies in Patient Samples |
| title_short | Comparison of VSV Pseudovirus and Focus Reduction Neutralization Assays for Measurement of Anti-Andes orthohantavirus Neutralizing Antibodies in Patient Samples |
| title_sort | comparison of vsv pseudovirus and focus reduction neutralization assays for measurement of anti andes orthohantavirus neutralizing antibodies in patient samples |
| topic | Andes orthohantavirus (ANDV) hantavirus cardiopulmonary syndrome (HCPS) orthohantavirus vesicular stomatitis virus (VSV) pseudovirus neutralizing antibodies |
| url | https://www.frontiersin.org/article/10.3389/fcimb.2020.00444/full |
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