Epitope mapping of mono- and polyclonal antibodies by screening phage-displayed random peptide libraries
Detailed knowledge of antigenic determinants is crucial when characterizing therapeutic and diagnostic antibodies, assessing vaccine effectiveness and developing epitope-based vaccines. Most epitope mapping approaches are labor intensive and costly. In this study, we evaluated panning of phage-displ...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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Slovenian Chemical Society
2016-10-01
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| Series: | Acta Chimica Slovenica |
| Subjects: | |
| Online Access: | https://journals.matheo.si/index.php/ACSi/article/view/2458 |
| _version_ | 1828870537841999872 |
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| author | Tomaž Bratkovič Peter Molek |
| author_facet | Tomaž Bratkovič Peter Molek |
| author_sort | Tomaž Bratkovič |
| collection | DOAJ |
| description | Detailed knowledge of antigenic determinants is crucial when characterizing therapeutic and diagnostic antibodies, assessing vaccine effectiveness and developing epitope-based vaccines. Most epitope mapping approaches are labor intensive and costly. In this study, we evaluated panning of phage-displayed random peptide libraries against antibodies as a tool for cognate epitope identification. We used six antibodies directed to three model protein antigens as targets to show that the approach is applicable to both mono- and polyclonal antibodies. The technique is well-suited especially for identification of linear epitopes. Mapping of conformational epitopes is more challenging, tends to be more subjective and requires use of computational tools. Nevertheless, when combined with functional data such as structure-activity relationship of antigen muteins, one can make reliable conformational epitope predictions based on phage display experiment data. As the described approach is fast and relatively inexpensive, we suggest it is employed early in antibody characterization and later validated by complementary methods. |
| first_indexed | 2024-12-13T06:14:10Z |
| format | Article |
| id | doaj.art-0c1ed979827648b7a4560a5884e71615 |
| institution | Directory Open Access Journal |
| issn | 1318-0207 1580-3155 |
| language | English |
| last_indexed | 2024-12-13T06:14:10Z |
| publishDate | 2016-10-01 |
| publisher | Slovenian Chemical Society |
| record_format | Article |
| series | Acta Chimica Slovenica |
| spelling | doaj.art-0c1ed979827648b7a4560a5884e716152022-12-21T23:57:01ZengSlovenian Chemical SocietyActa Chimica Slovenica1318-02071580-31552016-10-0163491491910.17344/acsi.2016.2458395Epitope mapping of mono- and polyclonal antibodies by screening phage-displayed random peptide librariesTomaž Bratkovič0Peter MolekUniversity of Ljubljana, Faculty of PharmacyDetailed knowledge of antigenic determinants is crucial when characterizing therapeutic and diagnostic antibodies, assessing vaccine effectiveness and developing epitope-based vaccines. Most epitope mapping approaches are labor intensive and costly. In this study, we evaluated panning of phage-displayed random peptide libraries against antibodies as a tool for cognate epitope identification. We used six antibodies directed to three model protein antigens as targets to show that the approach is applicable to both mono- and polyclonal antibodies. The technique is well-suited especially for identification of linear epitopes. Mapping of conformational epitopes is more challenging, tends to be more subjective and requires use of computational tools. Nevertheless, when combined with functional data such as structure-activity relationship of antigen muteins, one can make reliable conformational epitope predictions based on phage display experiment data. As the described approach is fast and relatively inexpensive, we suggest it is employed early in antibody characterization and later validated by complementary methods.https://journals.matheo.si/index.php/ACSi/article/view/2458peptideepitope mappingmimotopephage displayantibody |
| spellingShingle | Tomaž Bratkovič Peter Molek Epitope mapping of mono- and polyclonal antibodies by screening phage-displayed random peptide libraries Acta Chimica Slovenica peptide epitope mapping mimotope phage display antibody |
| title | Epitope mapping of mono- and polyclonal antibodies by screening phage-displayed random peptide libraries |
| title_full | Epitope mapping of mono- and polyclonal antibodies by screening phage-displayed random peptide libraries |
| title_fullStr | Epitope mapping of mono- and polyclonal antibodies by screening phage-displayed random peptide libraries |
| title_full_unstemmed | Epitope mapping of mono- and polyclonal antibodies by screening phage-displayed random peptide libraries |
| title_short | Epitope mapping of mono- and polyclonal antibodies by screening phage-displayed random peptide libraries |
| title_sort | epitope mapping of mono and polyclonal antibodies by screening phage displayed random peptide libraries |
| topic | peptide epitope mapping mimotope phage display antibody |
| url | https://journals.matheo.si/index.php/ACSi/article/view/2458 |
| work_keys_str_mv | AT tomazbratkovic epitopemappingofmonoandpolyclonalantibodiesbyscreeningphagedisplayedrandompeptidelibraries AT petermolek epitopemappingofmonoandpolyclonalantibodiesbyscreeningphagedisplayedrandompeptidelibraries |