Antibacterial substances from leaves of Protium spruceanum (Burseraceae): in vitro and in silico evaluation
Due to the increase of bacterial resistance, the search for new antibiotics is necessary and the medicinal plants represent its most important source. The aim of this study was to evaluate the antibacterial property of extract and fractions from Protium spruceanum leaves, against pathogenic bacteria...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Universidade de São Paulo
2021-04-01
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| Series: | Brazilian Journal of Pharmaceutical Sciences |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502020000100623&tlng=en |
| _version_ | 1811273408369917952 |
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| author | Tatiane Roquete Amparo Ivanildes Vasconcelos Rodrigues Janaína Brandão Seibert Tamires Cunha Almeida Vivette Appolinário Rodrigues Cabral Paula Melo de Abreu Vieira Geraldo Célio Brandão Mauro Lúcio Gonçalves de Oliveira Glenda Nicioli da Silva Orlando David Henrique dos Santos Sidney Augusto Vieira Filho Luiz Fernando Medeiros Teixeira Gustavo Henrique Bianco de Souza |
| author_facet | Tatiane Roquete Amparo Ivanildes Vasconcelos Rodrigues Janaína Brandão Seibert Tamires Cunha Almeida Vivette Appolinário Rodrigues Cabral Paula Melo de Abreu Vieira Geraldo Célio Brandão Mauro Lúcio Gonçalves de Oliveira Glenda Nicioli da Silva Orlando David Henrique dos Santos Sidney Augusto Vieira Filho Luiz Fernando Medeiros Teixeira Gustavo Henrique Bianco de Souza |
| author_sort | Tatiane Roquete Amparo |
| collection | DOAJ |
| description | Due to the increase of bacterial resistance, the search for new antibiotics is necessary and the medicinal plants represent its most important source. The aim of this study was to evaluate the antibacterial property of extract and fractions from Protium spruceanum leaves, against pathogenic bacteria. By means of diffusion and microdilution assays, the crude extract was active against the nine bacteria tested being the hydromethanolic fraction the most active. During phytochemical procedures, procyanidin (1) and catechin (2) were identified as the main antibacterial constituents of this fraction. In silico results obtained using PASSonline tool indicated 1 and 2 as having good potential to interact with different targets of currently used antibiotics. These results no indicated potential to none DNA effect and indicated the cell wall as mainly target. Electrophoresis result supported that had no DNA damage. Cell wall damage was confirmed by propidium iodide test that showed increased membrane permeability and by cell surface deformations observed in scanning electronic microscopy. The in vitro assays together with the in silico prediction results establish the potential of P. spruceanum as source of antibacterial compounds that acts on important bacterial targets. These results contribute to the development of natural substances against pathogenic bacteria and to discovery of new antibiotics. |
| first_indexed | 2024-04-12T22:58:36Z |
| format | Article |
| id | doaj.art-0c1fa0391f5447789a0a9aa469729f74 |
| institution | Directory Open Access Journal |
| issn | 2175-9790 |
| language | English |
| last_indexed | 2024-04-12T22:58:36Z |
| publishDate | 2021-04-01 |
| publisher | Universidade de São Paulo |
| record_format | Article |
| series | Brazilian Journal of Pharmaceutical Sciences |
| spelling | doaj.art-0c1fa0391f5447789a0a9aa469729f742022-12-22T03:13:07ZengUniversidade de São PauloBrazilian Journal of Pharmaceutical Sciences2175-97902021-04-015610.1590/s2175-97902020000118474Antibacterial substances from leaves of Protium spruceanum (Burseraceae): in vitro and in silico evaluationTatiane Roquete AmparoIvanildes Vasconcelos RodriguesJanaína Brandão SeibertTamires Cunha AlmeidaVivette Appolinário Rodrigues CabralPaula Melo de Abreu VieiraGeraldo Célio BrandãoMauro Lúcio Gonçalves de OliveiraGlenda Nicioli da SilvaOrlando David Henrique dos SantosSidney Augusto Vieira FilhoLuiz Fernando Medeiros TeixeiraGustavo Henrique Bianco de Souzahttps://orcid.org/0000-0002-1548-3274Due to the increase of bacterial resistance, the search for new antibiotics is necessary and the medicinal plants represent its most important source. The aim of this study was to evaluate the antibacterial property of extract and fractions from Protium spruceanum leaves, against pathogenic bacteria. By means of diffusion and microdilution assays, the crude extract was active against the nine bacteria tested being the hydromethanolic fraction the most active. During phytochemical procedures, procyanidin (1) and catechin (2) were identified as the main antibacterial constituents of this fraction. In silico results obtained using PASSonline tool indicated 1 and 2 as having good potential to interact with different targets of currently used antibiotics. These results no indicated potential to none DNA effect and indicated the cell wall as mainly target. Electrophoresis result supported that had no DNA damage. Cell wall damage was confirmed by propidium iodide test that showed increased membrane permeability and by cell surface deformations observed in scanning electronic microscopy. The in vitro assays together with the in silico prediction results establish the potential of P. spruceanum as source of antibacterial compounds that acts on important bacterial targets. These results contribute to the development of natural substances against pathogenic bacteria and to discovery of new antibiotics.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502020000100623&tlng=enProtium spruceanumBurseraceaeProcyanidinCatechinAntibacterial activity |
| spellingShingle | Tatiane Roquete Amparo Ivanildes Vasconcelos Rodrigues Janaína Brandão Seibert Tamires Cunha Almeida Vivette Appolinário Rodrigues Cabral Paula Melo de Abreu Vieira Geraldo Célio Brandão Mauro Lúcio Gonçalves de Oliveira Glenda Nicioli da Silva Orlando David Henrique dos Santos Sidney Augusto Vieira Filho Luiz Fernando Medeiros Teixeira Gustavo Henrique Bianco de Souza Antibacterial substances from leaves of Protium spruceanum (Burseraceae): in vitro and in silico evaluation Brazilian Journal of Pharmaceutical Sciences Protium spruceanum Burseraceae Procyanidin Catechin Antibacterial activity |
| title | Antibacterial substances from leaves of Protium spruceanum (Burseraceae): in vitro and in silico evaluation |
| title_full | Antibacterial substances from leaves of Protium spruceanum (Burseraceae): in vitro and in silico evaluation |
| title_fullStr | Antibacterial substances from leaves of Protium spruceanum (Burseraceae): in vitro and in silico evaluation |
| title_full_unstemmed | Antibacterial substances from leaves of Protium spruceanum (Burseraceae): in vitro and in silico evaluation |
| title_short | Antibacterial substances from leaves of Protium spruceanum (Burseraceae): in vitro and in silico evaluation |
| title_sort | antibacterial substances from leaves of protium spruceanum burseraceae in vitro and in silico evaluation |
| topic | Protium spruceanum Burseraceae Procyanidin Catechin Antibacterial activity |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502020000100623&tlng=en |
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