Rice UCL8, a plantacyanin gene targeted by miR408, regulates fertility by controlling pollen tube germination and growth
Abstract Background Pollen tube formation and growth are crucial steps that lead to seed production. Despite the importance of pollen tube growth, the molecular mechanisms implicated in its spatial and temporal control are not fully known. In this study, we found an uclacyanin gene, OsUCL8, that reg...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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SpringerOpen
2018-11-01
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| Series: | Rice |
| Online Access: | http://link.springer.com/article/10.1186/s12284-018-0253-y |
| _version_ | 1818275533183516672 |
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| author | Fan Zhang Yu-Chan Zhang Jin-Ping Zhang Yang Yu Yan-Fei Zhou Yan-Zhao Feng Yu-Wei Yang Meng-Qi Lei Huang He Jian-Pin Lian Yue-Qin Chen |
| author_facet | Fan Zhang Yu-Chan Zhang Jin-Ping Zhang Yang Yu Yan-Fei Zhou Yan-Zhao Feng Yu-Wei Yang Meng-Qi Lei Huang He Jian-Pin Lian Yue-Qin Chen |
| author_sort | Fan Zhang |
| collection | DOAJ |
| description | Abstract Background Pollen tube formation and growth are crucial steps that lead to seed production. Despite the importance of pollen tube growth, the molecular mechanisms implicated in its spatial and temporal control are not fully known. In this study, we found an uclacyanin gene, OsUCL8, that regulates pollen intine deposition and pollen tube growth. Findings The overexpression of OsUCL8 led to a striking irregularity in pollen tube growth and pollination and thus affected the seed setting rate in rice; many pollen tubes appeared to lose the ability to grow directly into the style. Conversely, plants with OsUCL8 knocked out and plants overexpressing miR408, a negative regulator of OsUCL8, had vigorous pollens with a higher germination rate. We further demonstrated that OsUCL8 mainly affects pollen intine formation. The addition of Vitamin B1 (VB1) significantly contributed to the germination of OXUCL8 pollen grains, suggesting that OsUCL8 could be associated with VB1 production. Using a yeast two-hybrid system, we revealed that OsUCL8 interacts with the protein OsPKIWI, a homolog of the Arabidopsis FNRL protein. We thus hypothesized that OsUCL8 might regulate the production of VB components by interacting with OsPKIWI. This study revealed a novel molecular mechanism of pollen tube growth regulation. Conclusions The rice plantacyanin family member OsUCL8 plays an important role in pollen tube formation and growth and, in turn, regulates fertility and the seed setting rate. |
| first_indexed | 2024-12-12T22:31:16Z |
| format | Article |
| id | doaj.art-0c1fadc8a2d34776aba0177b9b6ac0b5 |
| institution | Directory Open Access Journal |
| issn | 1939-8425 1939-8433 |
| language | English |
| last_indexed | 2024-12-12T22:31:16Z |
| publishDate | 2018-11-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Rice |
| spelling | doaj.art-0c1fadc8a2d34776aba0177b9b6ac0b52022-12-22T00:09:36ZengSpringerOpenRice1939-84251939-84332018-11-011111610.1186/s12284-018-0253-yRice UCL8, a plantacyanin gene targeted by miR408, regulates fertility by controlling pollen tube germination and growthFan Zhang0Yu-Chan Zhang1Jin-Ping Zhang2Yang Yu3Yan-Fei Zhou4Yan-Zhao Feng5Yu-Wei Yang6Meng-Qi Lei7Huang He8Jian-Pin Lian9Yue-Qin Chen10Guangdong Provincial Key Laboratory of Plant Resources, State Key Laboratory for Biocontrol, School of Life Sciences, Sun Yat-Sen UniversityGuangdong Provincial Key Laboratory of Plant Resources, State Key Laboratory for Biocontrol, School of Life Sciences, Sun Yat-Sen UniversityGuangdong Provincial Key Laboratory of Plant Resources, State Key Laboratory for Biocontrol, School of Life Sciences, Sun Yat-Sen UniversityGuangdong Provincial Key Laboratory of Plant Resources, State Key Laboratory for Biocontrol, School of Life Sciences, Sun Yat-Sen UniversityGuangdong Provincial Key Laboratory of Plant Resources, State Key Laboratory for Biocontrol, School of Life Sciences, Sun Yat-Sen UniversityGuangdong Provincial Key Laboratory of Plant Resources, State Key Laboratory for Biocontrol, School of Life Sciences, Sun Yat-Sen UniversityGuangdong Provincial Key Laboratory of Plant Resources, State Key Laboratory for Biocontrol, School of Life Sciences, Sun Yat-Sen UniversityGuangdong Provincial Key Laboratory of Plant Resources, State Key Laboratory for Biocontrol, School of Life Sciences, Sun Yat-Sen UniversityGuangdong Provincial Key Laboratory of Plant Resources, State Key Laboratory for Biocontrol, School of Life Sciences, Sun Yat-Sen UniversityGuangdong Provincial Key Laboratory of Plant Resources, State Key Laboratory for Biocontrol, School of Life Sciences, Sun Yat-Sen UniversityGuangdong Provincial Key Laboratory of Plant Resources, State Key Laboratory for Biocontrol, School of Life Sciences, Sun Yat-Sen UniversityAbstract Background Pollen tube formation and growth are crucial steps that lead to seed production. Despite the importance of pollen tube growth, the molecular mechanisms implicated in its spatial and temporal control are not fully known. In this study, we found an uclacyanin gene, OsUCL8, that regulates pollen intine deposition and pollen tube growth. Findings The overexpression of OsUCL8 led to a striking irregularity in pollen tube growth and pollination and thus affected the seed setting rate in rice; many pollen tubes appeared to lose the ability to grow directly into the style. Conversely, plants with OsUCL8 knocked out and plants overexpressing miR408, a negative regulator of OsUCL8, had vigorous pollens with a higher germination rate. We further demonstrated that OsUCL8 mainly affects pollen intine formation. The addition of Vitamin B1 (VB1) significantly contributed to the germination of OXUCL8 pollen grains, suggesting that OsUCL8 could be associated with VB1 production. Using a yeast two-hybrid system, we revealed that OsUCL8 interacts with the protein OsPKIWI, a homolog of the Arabidopsis FNRL protein. We thus hypothesized that OsUCL8 might regulate the production of VB components by interacting with OsPKIWI. This study revealed a novel molecular mechanism of pollen tube growth regulation. Conclusions The rice plantacyanin family member OsUCL8 plays an important role in pollen tube formation and growth and, in turn, regulates fertility and the seed setting rate.http://link.springer.com/article/10.1186/s12284-018-0253-y |
| spellingShingle | Fan Zhang Yu-Chan Zhang Jin-Ping Zhang Yang Yu Yan-Fei Zhou Yan-Zhao Feng Yu-Wei Yang Meng-Qi Lei Huang He Jian-Pin Lian Yue-Qin Chen Rice UCL8, a plantacyanin gene targeted by miR408, regulates fertility by controlling pollen tube germination and growth Rice |
| title | Rice UCL8, a plantacyanin gene targeted by miR408, regulates fertility by controlling pollen tube germination and growth |
| title_full | Rice UCL8, a plantacyanin gene targeted by miR408, regulates fertility by controlling pollen tube germination and growth |
| title_fullStr | Rice UCL8, a plantacyanin gene targeted by miR408, regulates fertility by controlling pollen tube germination and growth |
| title_full_unstemmed | Rice UCL8, a plantacyanin gene targeted by miR408, regulates fertility by controlling pollen tube germination and growth |
| title_short | Rice UCL8, a plantacyanin gene targeted by miR408, regulates fertility by controlling pollen tube germination and growth |
| title_sort | rice ucl8 a plantacyanin gene targeted by mir408 regulates fertility by controlling pollen tube germination and growth |
| url | http://link.springer.com/article/10.1186/s12284-018-0253-y |
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