Protective Role of Flavonoids against Intestinal Pro-Inflammatory Effects of Silver Nanoparticles
Silver nanoparticles (AgNP) have been increasingly incorporated into food-related and hygiene products for their unique antimicrobial and preservative properties. The consequent oral exposure may then result in unpredicted harmful effects in the gastrointestinal tract (GIT), which should be consider...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-10-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/26/21/6610 |
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| author | Ana T. Rufino Ana Ramalho Adelaide Sousa José Miguel P. Ferreira de Oliveira Paulo Freitas Manuel A. Gonzalez Gómez Yolanda Piñeiro-Redondo José Rivas Félix Carvalho Eduarda Fernandes Marisa Freitas |
| author_facet | Ana T. Rufino Ana Ramalho Adelaide Sousa José Miguel P. Ferreira de Oliveira Paulo Freitas Manuel A. Gonzalez Gómez Yolanda Piñeiro-Redondo José Rivas Félix Carvalho Eduarda Fernandes Marisa Freitas |
| author_sort | Ana T. Rufino |
| collection | DOAJ |
| description | Silver nanoparticles (AgNP) have been increasingly incorporated into food-related and hygiene products for their unique antimicrobial and preservative properties. The consequent oral exposure may then result in unpredicted harmful effects in the gastrointestinal tract (GIT), which should be considered in the risk assessment and risk management of these materials. In the present study, the toxic effects of polyethyleneimine (PEI)-coated AgNP (4 and 19 nm) were evaluated in GIT-relevant cells (Caco-2 cell line as a model of human intestinal cells, and neutrophils as a model of the intestinal inflammatory response). This study also evaluated the putative protective action of dietary flavonoids against such harmful effects. The obtained results showed that AgNP of 4 and 19 nm effectively induced Caco-2 cell death by apoptosis with concomitant production of nitric oxide, irrespective of the size. It was also observed that AgNP induced human neutrophil oxidative burst. Interestingly, some flavonoids, namely quercetin and quercetagetin, prevented the deleterious effects of AgNP in both cell types. Overall, the data of the present study provide a first insight into the promising protective role of flavonoids against the potentially toxic effects of AgNP at the intestinal level. |
| first_indexed | 2024-03-10T05:55:29Z |
| format | Article |
| id | doaj.art-0c2285f2ac8c488886f9b80afc590853 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T05:55:29Z |
| publishDate | 2021-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-0c2285f2ac8c488886f9b80afc5908532023-11-22T21:23:53ZengMDPI AGMolecules1420-30492021-10-012621661010.3390/molecules26216610Protective Role of Flavonoids against Intestinal Pro-Inflammatory Effects of Silver NanoparticlesAna T. Rufino0Ana Ramalho1Adelaide Sousa2José Miguel P. Ferreira de Oliveira3Paulo Freitas4Manuel A. Gonzalez Gómez5Yolanda Piñeiro-Redondo6José Rivas7Félix Carvalho8Eduarda Fernandes9Marisa Freitas10LAQV, REQUIMTE, Laboratory of Applied Chemistry, Department of Chemical Sciences, Faculty of Pharmacy, University of Porto, 4050-313 Porto, PortugalLAQV, REQUIMTE, Laboratory of Applied Chemistry, Department of Chemical Sciences, Faculty of Pharmacy, University of Porto, 4050-313 Porto, PortugalLAQV, REQUIMTE, Laboratory of Applied Chemistry, Department of Chemical Sciences, Faculty of Pharmacy, University of Porto, 4050-313 Porto, PortugalLAQV, REQUIMTE, Laboratory of Applied Chemistry, Department of Chemical Sciences, Faculty of Pharmacy, University of Porto, 4050-313 Porto, PortugalInternational Iberian Nanotechnology Laboratory, 4715-330 Braga, PortugalNanotechnology and Magnetism Lab—NANOMAG, Department of Applied Physics, Universidade de Santiago de Compostela, 15782 Santiago de Compostela, SpainNanotechnology and Magnetism Lab—NANOMAG, Department of Applied Physics, Universidade de Santiago de Compostela, 15782 Santiago de Compostela, SpainNanotechnology and Magnetism Lab—NANOMAG, Department of Applied Physics, Universidade de Santiago de Compostela, 15782 Santiago de Compostela, SpainUCIBIO, REQUIMTE, Laboratory of Toxicology, Department of Biological Sciences, Faculty of Pharmacy, University of Porto, 4050-313 Porto, PortugalLAQV, REQUIMTE, Laboratory of Applied Chemistry, Department of Chemical Sciences, Faculty of Pharmacy, University of Porto, 4050-313 Porto, PortugalLAQV, REQUIMTE, Laboratory of Applied Chemistry, Department of Chemical Sciences, Faculty of Pharmacy, University of Porto, 4050-313 Porto, PortugalSilver nanoparticles (AgNP) have been increasingly incorporated into food-related and hygiene products for their unique antimicrobial and preservative properties. The consequent oral exposure may then result in unpredicted harmful effects in the gastrointestinal tract (GIT), which should be considered in the risk assessment and risk management of these materials. In the present study, the toxic effects of polyethyleneimine (PEI)-coated AgNP (4 and 19 nm) were evaluated in GIT-relevant cells (Caco-2 cell line as a model of human intestinal cells, and neutrophils as a model of the intestinal inflammatory response). This study also evaluated the putative protective action of dietary flavonoids against such harmful effects. The obtained results showed that AgNP of 4 and 19 nm effectively induced Caco-2 cell death by apoptosis with concomitant production of nitric oxide, irrespective of the size. It was also observed that AgNP induced human neutrophil oxidative burst. Interestingly, some flavonoids, namely quercetin and quercetagetin, prevented the deleterious effects of AgNP in both cell types. Overall, the data of the present study provide a first insight into the promising protective role of flavonoids against the potentially toxic effects of AgNP at the intestinal level.https://www.mdpi.com/1420-3049/26/21/6610inflammationnanoparticlesintestinal cellsneutrophilsflavonoids |
| spellingShingle | Ana T. Rufino Ana Ramalho Adelaide Sousa José Miguel P. Ferreira de Oliveira Paulo Freitas Manuel A. Gonzalez Gómez Yolanda Piñeiro-Redondo José Rivas Félix Carvalho Eduarda Fernandes Marisa Freitas Protective Role of Flavonoids against Intestinal Pro-Inflammatory Effects of Silver Nanoparticles Molecules inflammation nanoparticles intestinal cells neutrophils flavonoids |
| title | Protective Role of Flavonoids against Intestinal Pro-Inflammatory Effects of Silver Nanoparticles |
| title_full | Protective Role of Flavonoids against Intestinal Pro-Inflammatory Effects of Silver Nanoparticles |
| title_fullStr | Protective Role of Flavonoids against Intestinal Pro-Inflammatory Effects of Silver Nanoparticles |
| title_full_unstemmed | Protective Role of Flavonoids against Intestinal Pro-Inflammatory Effects of Silver Nanoparticles |
| title_short | Protective Role of Flavonoids against Intestinal Pro-Inflammatory Effects of Silver Nanoparticles |
| title_sort | protective role of flavonoids against intestinal pro inflammatory effects of silver nanoparticles |
| topic | inflammation nanoparticles intestinal cells neutrophils flavonoids |
| url | https://www.mdpi.com/1420-3049/26/21/6610 |
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