Decellularized Extracellular Matrix Scaffolds for Cardiovascular Tissue Engineering: Current Techniques and Challenges
Cardiovascular diseases are the leading cause of global mortality. Over the past two decades, researchers have tried to provide novel solutions for end-stage heart failure to address cardiac transplantation hurdles such as donor organ shortage, chronic rejection, and life-long immunosuppression. Car...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-10-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/23/21/13040 |
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| author | Greta Ionela Barbulescu Florina Maria Bojin Valentin Laurentiu Ordodi Iacob Daniel Goje Andreea Severina Barbulescu Virgil Paunescu |
| author_facet | Greta Ionela Barbulescu Florina Maria Bojin Valentin Laurentiu Ordodi Iacob Daniel Goje Andreea Severina Barbulescu Virgil Paunescu |
| author_sort | Greta Ionela Barbulescu |
| collection | DOAJ |
| description | Cardiovascular diseases are the leading cause of global mortality. Over the past two decades, researchers have tried to provide novel solutions for end-stage heart failure to address cardiac transplantation hurdles such as donor organ shortage, chronic rejection, and life-long immunosuppression. Cardiac decellularized extracellular matrix (dECM) has been widely explored as a promising approach in tissue-regenerative medicine because of its remarkable similarity to the original tissue. Optimized decellularization protocols combining physical, chemical, and enzymatic agents have been developed to obtain the perfect balance between cell removal, ECM composition, and function maintenance. However, proper assessment of decellularized tissue composition is still needed before clinical translation. Recellularizing the acellular scaffold with organ-specific cells and evaluating the extent of cardiomyocyte repopulation is also challenging. This review aims to discuss the existing literature on decellularized cardiac scaffolds, especially on the advantages and methods of preparation, pointing out areas for improvement. Finally, an overview of the state of research regarding the application of cardiac dECM and future challenges in bioengineering a human heart suitable for transplantation is provided. |
| first_indexed | 2024-03-09T19:01:13Z |
| format | Article |
| id | doaj.art-0c241cb986544062a6fbf3448af32b79 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T19:01:13Z |
| publishDate | 2022-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-0c241cb986544062a6fbf3448af32b792023-11-24T05:01:21ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-10-0123211304010.3390/ijms232113040Decellularized Extracellular Matrix Scaffolds for Cardiovascular Tissue Engineering: Current Techniques and ChallengesGreta Ionela Barbulescu0Florina Maria Bojin1Valentin Laurentiu Ordodi2Iacob Daniel Goje3Andreea Severina Barbulescu4Virgil Paunescu5Immuno-Physiology and Biotechnologies Center (CIFBIOTEH), Department of Functional Sciences, “Victor Babes” University of Medicine and Pharmacy, No 2 Eftimie Murgu Square, 300041 Timisoara, RomaniaImmuno-Physiology and Biotechnologies Center (CIFBIOTEH), Department of Functional Sciences, “Victor Babes” University of Medicine and Pharmacy, No 2 Eftimie Murgu Square, 300041 Timisoara, RomaniaClinical Emergency County Hospital “Pius Brinzeu” Timisoara, Center for Gene and Cellular Therapies in the Treatment of Cancer Timisoara-OncoGen, No 156 Liviu Rebreanu, 300723 Timisoara, RomaniaDepartment of Medical Semiology I, “Victor Babes” University of Medicine and Pharmacy, No 2 Eftimie Murgu Square, 300041 Timisoara, RomaniaCenter for Advanced Research in Gastroenterology and Hepatology, Department of Internal Medicine II, Division of Gastroenterology and Hepatology, “Victor Babes” University of Medicine and Pharmacy, 300041 Timisoara, RomaniaImmuno-Physiology and Biotechnologies Center (CIFBIOTEH), Department of Functional Sciences, “Victor Babes” University of Medicine and Pharmacy, No 2 Eftimie Murgu Square, 300041 Timisoara, RomaniaCardiovascular diseases are the leading cause of global mortality. Over the past two decades, researchers have tried to provide novel solutions for end-stage heart failure to address cardiac transplantation hurdles such as donor organ shortage, chronic rejection, and life-long immunosuppression. Cardiac decellularized extracellular matrix (dECM) has been widely explored as a promising approach in tissue-regenerative medicine because of its remarkable similarity to the original tissue. Optimized decellularization protocols combining physical, chemical, and enzymatic agents have been developed to obtain the perfect balance between cell removal, ECM composition, and function maintenance. However, proper assessment of decellularized tissue composition is still needed before clinical translation. Recellularizing the acellular scaffold with organ-specific cells and evaluating the extent of cardiomyocyte repopulation is also challenging. This review aims to discuss the existing literature on decellularized cardiac scaffolds, especially on the advantages and methods of preparation, pointing out areas for improvement. Finally, an overview of the state of research regarding the application of cardiac dECM and future challenges in bioengineering a human heart suitable for transplantation is provided.https://www.mdpi.com/1422-0067/23/21/13040decellularizationrecellularization3D scaffoldextracellular matrixtissue engineeringregenerative medicine |
| spellingShingle | Greta Ionela Barbulescu Florina Maria Bojin Valentin Laurentiu Ordodi Iacob Daniel Goje Andreea Severina Barbulescu Virgil Paunescu Decellularized Extracellular Matrix Scaffolds for Cardiovascular Tissue Engineering: Current Techniques and Challenges International Journal of Molecular Sciences decellularization recellularization 3D scaffold extracellular matrix tissue engineering regenerative medicine |
| title | Decellularized Extracellular Matrix Scaffolds for Cardiovascular Tissue Engineering: Current Techniques and Challenges |
| title_full | Decellularized Extracellular Matrix Scaffolds for Cardiovascular Tissue Engineering: Current Techniques and Challenges |
| title_fullStr | Decellularized Extracellular Matrix Scaffolds for Cardiovascular Tissue Engineering: Current Techniques and Challenges |
| title_full_unstemmed | Decellularized Extracellular Matrix Scaffolds for Cardiovascular Tissue Engineering: Current Techniques and Challenges |
| title_short | Decellularized Extracellular Matrix Scaffolds for Cardiovascular Tissue Engineering: Current Techniques and Challenges |
| title_sort | decellularized extracellular matrix scaffolds for cardiovascular tissue engineering current techniques and challenges |
| topic | decellularization recellularization 3D scaffold extracellular matrix tissue engineering regenerative medicine |
| url | https://www.mdpi.com/1422-0067/23/21/13040 |
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