OsFH13, a type I formin, is indispensable for rice morphogenesis
Actin binding proteins regulate plant morphogenesis by regulating actin dynamics and formins affect plant morphogenesis by modulating cytoskeleton dynamics. However, the roles of formins in rice morphogenesis remain largely unclear. Here, we report the biochemical and biological functions of formin...
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| Format: | Article |
| Language: | English |
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KeAi Communications Co. Ltd.
2022-06-01
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| Series: | Reproduction and Breeding |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S266707122200014X |
| _version_ | 1797936285224009728 |
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| author | Anran Ren Jiao Zhang Zengyu Liu Pingzhou Du Fengli Zhang Haiyun Ren Dabing Zhang |
| author_facet | Anran Ren Jiao Zhang Zengyu Liu Pingzhou Du Fengli Zhang Haiyun Ren Dabing Zhang |
| author_sort | Anran Ren |
| collection | DOAJ |
| description | Actin binding proteins regulate plant morphogenesis by regulating actin dynamics and formins affect plant morphogenesis by modulating cytoskeleton dynamics. However, the roles of formins in rice morphogenesis remain largely unclear. Here, we report the biochemical and biological functions of formin homolog 13 (OsFH13), one type I formin, in regulating rice morphogenesis. OsFH13 was found to be ubiquitously expressed in both vegetative and reproductive organs including roots, stems, leaves, and flowers; while OsFH13 was found to be localized on the plasma membrane. The osfh13 mutant displayed pleiotropic phenotypes including reduced plant height, shorter roots, shorter stems, shrank flag leaves, and smaller seeds due to defective cell elongation and expansion. In the absence of OsFH13, actin filaments arrays were found to be unstable both in vivo and in vitro. In addition, OsFH13 was also found to be able to bundle microtubules in vitro. These results demonstrate that OsFH13 is critical to rice morphogenesis via stabilizing actin network, which could be useful to breed rice with desired morphogenesis. |
| first_indexed | 2024-04-10T18:28:26Z |
| format | Article |
| id | doaj.art-0c24a8f87b0b4ab6810d5a724715a165 |
| institution | Directory Open Access Journal |
| issn | 2667-0712 |
| language | English |
| last_indexed | 2024-04-10T18:28:26Z |
| publishDate | 2022-06-01 |
| publisher | KeAi Communications Co. Ltd. |
| record_format | Article |
| series | Reproduction and Breeding |
| spelling | doaj.art-0c24a8f87b0b4ab6810d5a724715a1652023-02-02T04:50:46ZengKeAi Communications Co. Ltd.Reproduction and Breeding2667-07122022-06-01224655OsFH13, a type I formin, is indispensable for rice morphogenesisAnran Ren0Jiao Zhang1Zengyu Liu2Pingzhou Du3Fengli Zhang4Haiyun Ren5Dabing Zhang6Joint International Research Laboratory of Metabolic and Developmental Sciences, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, 200240, ChinaJoint International Research Laboratory of Metabolic and Developmental Sciences, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, 200240, ChinaJoint International Research Laboratory of Metabolic and Developmental Sciences, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, 200240, ChinaCenter for Biological Science and Technology, Guangdong Zhuhai-Macao Joint Biotech Laboratory, Advanced Institute of Natural Science, Beijing Normal University, Zhuhai, 519087, ChinaJoint International Research Laboratory of Metabolic and Developmental Sciences, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, 200240, ChinaCenter for Biological Science and Technology, Guangdong Zhuhai-Macao Joint Biotech Laboratory, Advanced Institute of Natural Science, Beijing Normal University, Zhuhai, 519087, China; Corresponding author.Joint International Research Laboratory of Metabolic and Developmental Sciences, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, 200240, China; Corresponding author.Actin binding proteins regulate plant morphogenesis by regulating actin dynamics and formins affect plant morphogenesis by modulating cytoskeleton dynamics. However, the roles of formins in rice morphogenesis remain largely unclear. Here, we report the biochemical and biological functions of formin homolog 13 (OsFH13), one type I formin, in regulating rice morphogenesis. OsFH13 was found to be ubiquitously expressed in both vegetative and reproductive organs including roots, stems, leaves, and flowers; while OsFH13 was found to be localized on the plasma membrane. The osfh13 mutant displayed pleiotropic phenotypes including reduced plant height, shorter roots, shorter stems, shrank flag leaves, and smaller seeds due to defective cell elongation and expansion. In the absence of OsFH13, actin filaments arrays were found to be unstable both in vivo and in vitro. In addition, OsFH13 was also found to be able to bundle microtubules in vitro. These results demonstrate that OsFH13 is critical to rice morphogenesis via stabilizing actin network, which could be useful to breed rice with desired morphogenesis.http://www.sciencedirect.com/science/article/pii/S266707122200014XOsFH13MorphogenesisType I forminActin filamentsRice |
| spellingShingle | Anran Ren Jiao Zhang Zengyu Liu Pingzhou Du Fengli Zhang Haiyun Ren Dabing Zhang OsFH13, a type I formin, is indispensable for rice morphogenesis Reproduction and Breeding OsFH13 Morphogenesis Type I formin Actin filaments Rice |
| title | OsFH13, a type I formin, is indispensable for rice morphogenesis |
| title_full | OsFH13, a type I formin, is indispensable for rice morphogenesis |
| title_fullStr | OsFH13, a type I formin, is indispensable for rice morphogenesis |
| title_full_unstemmed | OsFH13, a type I formin, is indispensable for rice morphogenesis |
| title_short | OsFH13, a type I formin, is indispensable for rice morphogenesis |
| title_sort | osfh13 a type i formin is indispensable for rice morphogenesis |
| topic | OsFH13 Morphogenesis Type I formin Actin filaments Rice |
| url | http://www.sciencedirect.com/science/article/pii/S266707122200014X |
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