Evaluation of Analgesic Activity of Extracts of Delphinium denudatum in Animal Models: A Dose-dependent Pre-clinical Trial
Introduction: Pain is a common symptom of majority of clinical disorders which brings patients to a physician. Many traditional medicines have been introduced for relieving the pain and one such herb, Delphinium denudatum, is claimed to have an analgesic effect. Aim: To evaluate the analgesic a...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
JCDR Research and Publications Private Limited
2018-12-01
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| Series: | Journal of Clinical and Diagnostic Research |
| Subjects: | |
| Online Access: | https://jcdr.net/articles/PDF/12322/37415_CE[Ra1]_F(SL)_PF1(AJ_SL)_GR(SU)_PN(P).pdf |
| Summary: | Introduction: Pain is a common symptom of majority of
clinical disorders which brings patients to a physician. Many
traditional medicines have been introduced for relieving the
pain and one such herb, Delphinium denudatum, is claimed to
have an analgesic effect.
Aim: To evaluate the analgesic activity of ethanolic extract and
methanol fraction of Delphinium denudatum on Wistar albino rats.
Materials and Methods: This experimental study was carried
out in the Department of Pharmacology, JN Medical College,
Aligarh Muslim University, Aligarh, Uttar Pradesh, India. The
analgesic activity was evaluated by employing the Eddy’s Hot
Plate method and Tail Flick responsemethod (Orchid Scientifics,
India). In both the tests, Rats of either sex weighing 150-200 g
were used. The total number of animals n=36 were allocated
to six groups consisting of six animals each. Group I received
propylene glycol 0.3 mL/100g p.o., Group II received pentazocine
30 mg/kg i.p, Group III received ethanolic extract of Delphinium
denudatum 300 mg/kg p.o., Group IV received ethanolic extract
of Delphinium denudatum 600 mg/kg p.o., Group V received
methanol fraction of Delphinium denudatum 200 mg/kg p.o.,
Group VI received methanol fraction of Delphinium denudatum
400 mg/kg p.o. The response noted in animals who were tested
by hot plate method, was reaction time for licking/biting of both
the paws before and after administration of control and test
drugs. However, in Tail flick test, the pain threshold response
was recorded before and after administration of control and
test drugs. The statistical analysis was done by using one-way
ANOVA. The data are expressed as Mean±SEM. p<0.05 was
considered to be statistically significant.
Results: A significant analgesia was produced in all the
treatment groups when compared with control group in both the
test models. A dose-dependent significant (p<0.001) analgesia
was recorded in all the groups received ethanolic extracts
and methanol fraction of Delphinium denudatum. However, a
significant increase in reaction time and pain threshold in both
the test models was observed in the groups who were given
a higher dose of ethanolic extracts and methanol fraction of
Delphinium denudatum. Interestingly, this study noted an
approximately parallel degree of significant analgesia with a
group who received extract of Delphinium denudatum in a dose
of 600 mg/kg to a pentazocine group.
Conclusion: The present study reveals the dose-dependent
significant analgesic activity of the extracts of Delphinium
denudatum in both the test. However, the degree of analgesia
was recorded significantly higher in groups that received higher
doses of extracts of Delphinium denudatum. |
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| ISSN: | 2249-782X 0973-709X |