Carbon and nitrogen substrate utilization by archival <it>Salmonella typhimurium</it> LT2 cells
<p>Abstract</p> <p>Background</p> <p>A collection of over 20,000 <it>Salmonella typhimurium</it> LT2 mutants, sealed for four decades in agar stabs, is a unique resource for study of genetic and evolutionary changes. Previously, we reported extensive diversi...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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BMC
2002-09-01
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| Series: | BMC Evolutionary Biology |
| Online Access: | http://www.biomedcentral.com/1471-2148/2/14 |
| _version_ | 1798028377777504256 |
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| author | Edwards Kelly K Tracy Breca S Eisenstark Abraham |
| author_facet | Edwards Kelly K Tracy Breca S Eisenstark Abraham |
| author_sort | Edwards Kelly K |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>A collection of over 20,000 <it>Salmonella typhimurium</it> LT2 mutants, sealed for four decades in agar stabs, is a unique resource for study of genetic and evolutionary changes. Previously, we reported extensive diversity among descendants including diversity in RpoS and catalase synthesis, diversity in genome size, protein content, and reversion from auxotrophy to prototrophy.</p> <p>Results</p> <p>Extensive and variable losses and a few gains of catabolic functions were observed by this standardized method. Thus, 95 catabolic reactions were scored in each of three plates in wells containing specific carbon and nitrogen substrates.</p> <p>Conclusion</p> <p>While the phenotype microarray did not reveal a distinct pattern of mutation among the archival isolates, the data did confirm that various isolates have used multiple strategies to survive in the archival environment. Data from the MacConkey plates verified the changes in carbohydrate metabolism observed in the Biolog™ system.</p> |
| first_indexed | 2024-04-11T19:08:05Z |
| format | Article |
| id | doaj.art-0c257fd5effc43ccba03d663951ee8cf |
| institution | Directory Open Access Journal |
| issn | 1471-2148 |
| language | English |
| last_indexed | 2024-04-11T19:08:05Z |
| publishDate | 2002-09-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Evolutionary Biology |
| spelling | doaj.art-0c257fd5effc43ccba03d663951ee8cf2022-12-22T04:07:42ZengBMCBMC Evolutionary Biology1471-21482002-09-01211410.1186/1471-2148-2-14Carbon and nitrogen substrate utilization by archival <it>Salmonella typhimurium</it> LT2 cellsEdwards Kelly KTracy Breca SEisenstark Abraham<p>Abstract</p> <p>Background</p> <p>A collection of over 20,000 <it>Salmonella typhimurium</it> LT2 mutants, sealed for four decades in agar stabs, is a unique resource for study of genetic and evolutionary changes. Previously, we reported extensive diversity among descendants including diversity in RpoS and catalase synthesis, diversity in genome size, protein content, and reversion from auxotrophy to prototrophy.</p> <p>Results</p> <p>Extensive and variable losses and a few gains of catabolic functions were observed by this standardized method. Thus, 95 catabolic reactions were scored in each of three plates in wells containing specific carbon and nitrogen substrates.</p> <p>Conclusion</p> <p>While the phenotype microarray did not reveal a distinct pattern of mutation among the archival isolates, the data did confirm that various isolates have used multiple strategies to survive in the archival environment. Data from the MacConkey plates verified the changes in carbohydrate metabolism observed in the Biolog™ system.</p>http://www.biomedcentral.com/1471-2148/2/14 |
| spellingShingle | Edwards Kelly K Tracy Breca S Eisenstark Abraham Carbon and nitrogen substrate utilization by archival <it>Salmonella typhimurium</it> LT2 cells BMC Evolutionary Biology |
| title | Carbon and nitrogen substrate utilization by archival <it>Salmonella typhimurium</it> LT2 cells |
| title_full | Carbon and nitrogen substrate utilization by archival <it>Salmonella typhimurium</it> LT2 cells |
| title_fullStr | Carbon and nitrogen substrate utilization by archival <it>Salmonella typhimurium</it> LT2 cells |
| title_full_unstemmed | Carbon and nitrogen substrate utilization by archival <it>Salmonella typhimurium</it> LT2 cells |
| title_short | Carbon and nitrogen substrate utilization by archival <it>Salmonella typhimurium</it> LT2 cells |
| title_sort | carbon and nitrogen substrate utilization by archival it salmonella typhimurium it lt2 cells |
| url | http://www.biomedcentral.com/1471-2148/2/14 |
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