Secondary metabolite induced tolerance to Fusarium oxysporum f.sp. cubense TR4 in banana cv. Grand Naine through in vitro bio-immunization: a prospective research translation from induction to field tolerance
An innovative tissue culture mediated incorporation of metabolite-based biomolecule (Bio-immune) at in vitro stage itself in banana cv. Grand Naine was developed and validated for the production of Fusarium oxysporum f.sp. cubense TR4 tolerant plantlets. The novel bio-immune formulation developed by...
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2023-11-01
|
| Series: | Frontiers in Microbiology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1233469/full |
| _version_ | 1827631463271497728 |
|---|---|
| author | T Damodaran T Damodaran Maneesh Mishra M Muthukumar Shailendra Rajan Kavita Yadav Alok Kumar Prasenjit Debnath Sangeeta Kumari Popy Bora Ram Gopal Ram Gopal Sandeep Kumar |
| author_facet | T Damodaran T Damodaran Maneesh Mishra M Muthukumar Shailendra Rajan Kavita Yadav Alok Kumar Prasenjit Debnath Sangeeta Kumari Popy Bora Ram Gopal Ram Gopal Sandeep Kumar |
| author_sort | T Damodaran |
| collection | DOAJ |
| description | An innovative tissue culture mediated incorporation of metabolite-based biomolecule (Bio-immune) at in vitro stage itself in banana cv. Grand Naine was developed and validated for the production of Fusarium oxysporum f.sp. cubense TR4 tolerant plantlets. The novel bio-immune formulation developed by us, exhibited a significant antifungal potency against Foc TR4 with a high percent inhibition (100%) at a 2.5% concentration of bio-immune on the 5th, 7th, and 9th DAI. Bio-immune integrated during in vitro shoot proliferation stage in banana cv. Grand Naine recorded significant enhancement in the growth of roots and shoots. Bio-immune (0.5%) fortified media produced 12.67 shoots per clump whereas control registered only 9.67 shoots per clump. Similarly, maximum root numbers (7.67) were observed in bio-immune plants which were significantly higher over control (5.0). The bio-immunized banana transplants recorded a higher survival rate (97.57%) during acclimatization as compared to the control (94.53%). Furthermore, evaluation of the bio-immunized plants in pot experiments revealed that unimmunized plants treated with FocTR4 (TF) exhibited mortality between 60 and 90 days. On the 90th day after planting, a high mean disease severity index (DSI) of 3.45 was observed with unimmunized plantlets while the bio-immunized plants (TFBI) and ICAR-FUSICONT treated plants (TFTR) showed substantially reduced DSI (0.20 and 1.00) compared to FocTR4 treated control (TF). Significant increases in polyphenol oxidase (PPO), peroxidase (POD), β-1,3-glucanase, phenylalanine ammonia-lyase (PAL), chitinase activities, and enhanced phenol contents were recorded in bio-immunized plants compared to unimmunized plants. Field experiments at two different locations in Bihar, India revealed that bunch weight, no. of hands/bunch, and no. of fingers/hand of bio-immune treated plants were significantly higher compared to the control. |
| first_indexed | 2024-03-09T14:21:56Z |
| format | Article |
| id | doaj.art-0c259b11babf4928bbfb6e17246535a9 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-03-09T14:21:56Z |
| publishDate | 2023-11-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-0c259b11babf4928bbfb6e17246535a92023-11-28T10:02:28ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2023-11-011410.3389/fmicb.2023.12334691233469Secondary metabolite induced tolerance to Fusarium oxysporum f.sp. cubense TR4 in banana cv. Grand Naine through in vitro bio-immunization: a prospective research translation from induction to field toleranceT Damodaran0T Damodaran1Maneesh Mishra2M Muthukumar3Shailendra Rajan4Kavita Yadav5Alok Kumar6Prasenjit Debnath7Sangeeta Kumari8Popy Bora9Ram Gopal10Ram Gopal11Sandeep Kumar12ICAR-Central Institute for Subtropical Horticulture, Lucknow, Uttar Pradesh, IndiaICAR-Central Soil Salinity Research Institute, Lucknow, Uttar Pradesh, IndiaICAR-Central Institute for Subtropical Horticulture, Lucknow, Uttar Pradesh, IndiaICAR-Central Institute for Subtropical Horticulture, Lucknow, Uttar Pradesh, IndiaICAR-Central Institute for Subtropical Horticulture, Lucknow, Uttar Pradesh, IndiaICAR-Central Institute for Subtropical Horticulture, Lucknow, Uttar Pradesh, IndiaAssam Agricultural University, Jorhat, Assam, IndiaICAR-Central Soil Salinity Research Institute, Lucknow, Uttar Pradesh, IndiaICAR-Central Institute for Subtropical Horticulture, Lucknow, Uttar Pradesh, IndiaAssam Agricultural University, Jorhat, Assam, IndiaICAR-Central Soil Salinity Research Institute, Lucknow, Uttar Pradesh, IndiaKrishi Vigyan Kendra, Masodha, Acharya Narendra Deva University of Agriculture and Technology, Kumarganj, Ayodhya, Uttar Pradesh, IndiaICAR-Central Institute for Subtropical Horticulture, Lucknow, Uttar Pradesh, IndiaAn innovative tissue culture mediated incorporation of metabolite-based biomolecule (Bio-immune) at in vitro stage itself in banana cv. Grand Naine was developed and validated for the production of Fusarium oxysporum f.sp. cubense TR4 tolerant plantlets. The novel bio-immune formulation developed by us, exhibited a significant antifungal potency against Foc TR4 with a high percent inhibition (100%) at a 2.5% concentration of bio-immune on the 5th, 7th, and 9th DAI. Bio-immune integrated during in vitro shoot proliferation stage in banana cv. Grand Naine recorded significant enhancement in the growth of roots and shoots. Bio-immune (0.5%) fortified media produced 12.67 shoots per clump whereas control registered only 9.67 shoots per clump. Similarly, maximum root numbers (7.67) were observed in bio-immune plants which were significantly higher over control (5.0). The bio-immunized banana transplants recorded a higher survival rate (97.57%) during acclimatization as compared to the control (94.53%). Furthermore, evaluation of the bio-immunized plants in pot experiments revealed that unimmunized plants treated with FocTR4 (TF) exhibited mortality between 60 and 90 days. On the 90th day after planting, a high mean disease severity index (DSI) of 3.45 was observed with unimmunized plantlets while the bio-immunized plants (TFBI) and ICAR-FUSICONT treated plants (TFTR) showed substantially reduced DSI (0.20 and 1.00) compared to FocTR4 treated control (TF). Significant increases in polyphenol oxidase (PPO), peroxidase (POD), β-1,3-glucanase, phenylalanine ammonia-lyase (PAL), chitinase activities, and enhanced phenol contents were recorded in bio-immunized plants compared to unimmunized plants. Field experiments at two different locations in Bihar, India revealed that bunch weight, no. of hands/bunch, and no. of fingers/hand of bio-immune treated plants were significantly higher compared to the control.https://www.frontiersin.org/articles/10.3389/fmicb.2023.1233469/fullFusarium oxysporum f.sp. cubense TR4bananawilt diseasetissue culturebioimmuneLC-MS |
| spellingShingle | T Damodaran T Damodaran Maneesh Mishra M Muthukumar Shailendra Rajan Kavita Yadav Alok Kumar Prasenjit Debnath Sangeeta Kumari Popy Bora Ram Gopal Ram Gopal Sandeep Kumar Secondary metabolite induced tolerance to Fusarium oxysporum f.sp. cubense TR4 in banana cv. Grand Naine through in vitro bio-immunization: a prospective research translation from induction to field tolerance Frontiers in Microbiology Fusarium oxysporum f.sp. cubense TR4 banana wilt disease tissue culture bioimmune LC-MS |
| title | Secondary metabolite induced tolerance to Fusarium oxysporum f.sp. cubense TR4 in banana cv. Grand Naine through in vitro bio-immunization: a prospective research translation from induction to field tolerance |
| title_full | Secondary metabolite induced tolerance to Fusarium oxysporum f.sp. cubense TR4 in banana cv. Grand Naine through in vitro bio-immunization: a prospective research translation from induction to field tolerance |
| title_fullStr | Secondary metabolite induced tolerance to Fusarium oxysporum f.sp. cubense TR4 in banana cv. Grand Naine through in vitro bio-immunization: a prospective research translation from induction to field tolerance |
| title_full_unstemmed | Secondary metabolite induced tolerance to Fusarium oxysporum f.sp. cubense TR4 in banana cv. Grand Naine through in vitro bio-immunization: a prospective research translation from induction to field tolerance |
| title_short | Secondary metabolite induced tolerance to Fusarium oxysporum f.sp. cubense TR4 in banana cv. Grand Naine through in vitro bio-immunization: a prospective research translation from induction to field tolerance |
| title_sort | secondary metabolite induced tolerance to fusarium oxysporum f sp cubense tr4 in banana cv grand naine through in vitro bio immunization a prospective research translation from induction to field tolerance |
| topic | Fusarium oxysporum f.sp. cubense TR4 banana wilt disease tissue culture bioimmune LC-MS |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1233469/full |
| work_keys_str_mv | AT tdamodaran secondarymetaboliteinducedtolerancetofusariumoxysporumfspcubensetr4inbananacvgrandnainethroughinvitrobioimmunizationaprospectiveresearchtranslationfrominductiontofieldtolerance AT tdamodaran secondarymetaboliteinducedtolerancetofusariumoxysporumfspcubensetr4inbananacvgrandnainethroughinvitrobioimmunizationaprospectiveresearchtranslationfrominductiontofieldtolerance AT maneeshmishra secondarymetaboliteinducedtolerancetofusariumoxysporumfspcubensetr4inbananacvgrandnainethroughinvitrobioimmunizationaprospectiveresearchtranslationfrominductiontofieldtolerance AT mmuthukumar secondarymetaboliteinducedtolerancetofusariumoxysporumfspcubensetr4inbananacvgrandnainethroughinvitrobioimmunizationaprospectiveresearchtranslationfrominductiontofieldtolerance AT shailendrarajan secondarymetaboliteinducedtolerancetofusariumoxysporumfspcubensetr4inbananacvgrandnainethroughinvitrobioimmunizationaprospectiveresearchtranslationfrominductiontofieldtolerance AT kavitayadav secondarymetaboliteinducedtolerancetofusariumoxysporumfspcubensetr4inbananacvgrandnainethroughinvitrobioimmunizationaprospectiveresearchtranslationfrominductiontofieldtolerance AT alokkumar secondarymetaboliteinducedtolerancetofusariumoxysporumfspcubensetr4inbananacvgrandnainethroughinvitrobioimmunizationaprospectiveresearchtranslationfrominductiontofieldtolerance AT prasenjitdebnath secondarymetaboliteinducedtolerancetofusariumoxysporumfspcubensetr4inbananacvgrandnainethroughinvitrobioimmunizationaprospectiveresearchtranslationfrominductiontofieldtolerance AT sangeetakumari secondarymetaboliteinducedtolerancetofusariumoxysporumfspcubensetr4inbananacvgrandnainethroughinvitrobioimmunizationaprospectiveresearchtranslationfrominductiontofieldtolerance AT popybora secondarymetaboliteinducedtolerancetofusariumoxysporumfspcubensetr4inbananacvgrandnainethroughinvitrobioimmunizationaprospectiveresearchtranslationfrominductiontofieldtolerance AT ramgopal secondarymetaboliteinducedtolerancetofusariumoxysporumfspcubensetr4inbananacvgrandnainethroughinvitrobioimmunizationaprospectiveresearchtranslationfrominductiontofieldtolerance AT ramgopal secondarymetaboliteinducedtolerancetofusariumoxysporumfspcubensetr4inbananacvgrandnainethroughinvitrobioimmunizationaprospectiveresearchtranslationfrominductiontofieldtolerance AT sandeepkumar secondarymetaboliteinducedtolerancetofusariumoxysporumfspcubensetr4inbananacvgrandnainethroughinvitrobioimmunizationaprospectiveresearchtranslationfrominductiontofieldtolerance |